PMID- 30498928
OWN - NLM
STAT- In-Data-Review
LR  - 20190226
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 64
IP  - 3
DP  - 2019 Mar
TI  - BATF Interference Blocks Th17 Cell Differentiation and Inflammatory Response in
      Hepatitis B Virus Transgenic Mice.
PG  - 773-780
LID - 10.1007/s10620-018-5392-x [doi]
AB  - BACKGROUND: B cell-activating transcription factor (BATF) contributes to Th17
      cell differentiation and pathological inflammatory responses. AIMS: This study
      explored BATF as a regulator of Th17 differentiation in normal and hepatitis B
      virus (HBV) transgenic mice. METHODS: Normal mice were divided into control,
      short hairpin RNA (shRNA) scramble, and shRNA BATF groups. HBV transgenic mice
      were divided into control, entecavir, shRNA scramble, entecavir + vector control,
      entecavir + shRNA scramble, shRNA BATF, and entecavir + shRNA BATF groups. Serum 
      concentrations of AST, ALT, HBV-DNA, BATF, IL-17, and IL-22 and Th17 cell
      frequencies in the liver were compared among the groups. Correlations of serum
      HBV surface antigen (HBsAg), e-antigen (HBeAg), and core antigen (HBcAg)
      concentrations with BATF mRNA expression and the proportion of Th17 cells in the 
      livers of HBV transgenic mice were also analyzed. RESULTS: Serum AST, ALT, BATF, 
      IL-17, and IL-22 concentrations and Th17 cell proportions were higher in HBV
      transgenic mice relative to normal controls. Positive correlations of the HBcAg
      concentration with BATF mRNA and the proportion of Th17 cells were observed in
      HBV transgenic mice. BATF interference reduced the proportion of Th17 cells and
      serum IL-17 and IL-22 concentrations and led to obvious downregulation of AST,
      ALT, BATF, IL-17, and IL-22 expression and a reduced proportion of Th17 cells
      when combined with entecavir. CONCLUSION: HBV markedly upregulated BATF
      expression and promoted Th17 cell activation. By contrast, BATF interference
      significantly impeded the proliferation of Th17 cells and secretion of IL-17 and 
      IL-22 while alleviating hepatic lesions.
FAU - Chen, Long-Yan
AU  - Chen LY
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China.
FAU - Fan, Xiao-Peng
AU  - Fan XP
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China.
FAU - Fan, Yu-Chen
AU  - Fan YC
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China.
FAU - Zhao, Jing
AU  - Zhao J
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China.
FAU - Gao, Shuai
AU  - Gao S
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China.
FAU - Li, Feng
AU  - Li F
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China.
FAU - Qi, Zhao-Xia
AU  - Qi ZX
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China.
FAU - Wang, Kai
AU  - Wang K
AD  - Department of Hepatology, Qilu Hospital of Shandong University, Jinan, 250012,
      China. wangdoc876@126.com.
AD  - Institute of Hepatology, Shandong University, Wenhuaxi Road 107#, Jinan, 250012, 
      China. wangdoc876@126.com.
LA  - eng
GR  - 81171579/National Natural Science Foundation of China
GR  - 81201287/National Natural Science Foundation of China
GR  - 81300318/National Natural Science Foundation of China
GR  - 81371832/National Natural Science Foundation of China
GR  - 2012ZX10002007/Key Project of Chinese Ministry of Science and Technology
GR  - 2013ZX10002001/Key Project of Chinese Ministry of Science and Technology
GR  - 2014GSF118068/Science and Technology Development Plan of Shandong Province
PT  - Journal Article
DEP - 20181129
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
OTO - NOTNLM
OT  - BATF
OT  - HBV
OT  - Th17
OT  - Transgenic mice
EDAT- 2018/12/01 06:00
MHDA- 2018/12/01 06:00
CRDT- 2018/12/01 06:00
PHST- 2018/03/20 00:00 [received]
PHST- 2018/11/22 00:00 [accepted]
PHST- 2018/12/01 06:00 [pubmed]
PHST- 2018/12/01 06:00 [medline]
PHST- 2018/12/01 06:00 [entrez]
AID - 10.1007/s10620-018-5392-x [doi]
AID - 10.1007/s10620-018-5392-x [pii]
PST - ppublish
SO  - Dig Dis Sci. 2019 Mar;64(3):773-780. doi: 10.1007/s10620-018-5392-x. Epub 2018
      Nov 29.