PMID- 30275089
OWN - NLM
STAT- In-Data-Review
LR  - 20181217
IS  - 1098-6596 (Electronic)
IS  - 0066-4804 (Linking)
VI  - 62
IP  - 12
DP  - 2018 Dec
TI  - Emergence and Within-Host Genetic Evolution of Methicillin-Resistant
      Staphylococcus aureus Resistant to Linezolid in a Cystic Fibrosis Patient.
LID - e00720-18 [pii]
LID - 10.1128/AAC.00720-18 [doi]
AB  - Methicillin-resistant Staphylococcus aureus (MRSA) infection has increased in
      recent years among cystic fibrosis (CF) patients. Linezolid (LZD) is one of the
      antistaphylococcal antibiotics widely used in this context. Although LZD
      resistance is rare, it has been described as often associated with long-term
      treatments. Thirteen MRSA strains isolated over 5 years from one CF patient were 
      studied for LZD resistance emergence and subjected to whole-genome sequencing
      (WGS). Resistance emerged after three 15-day LZD therapeutic regimens over 4
      months. It was associated with the mutation of G to T at position 2576 (G2576T)
      in all 5 rrl copies, along with a very high MIC (>256 mg/liter) and a strong
      increase in the generation time. Resistant strains isolated during the ensuing
      LZD therapeutic regimens and until 13 months after LZD stopped harbored only 3 or
      4 mutated rrl copies, associated with lower MICs (8 to 32 mg/liter) and low to
      moderate generation time increases. Despite these differences, whole-genome
      sequencing allowed us to determine that all isolates, including the susceptible
      one isolated before LZD treatment, belonged to the same lineage. In conclusion,
      LZD resistance can emerge rapidly in CF patients and persist without linezolid
      selective pressure in colonizing MRSA strains belonging to the same lineage.
CI  - Copyright (c) 2018 American Society for Microbiology.
FAU - Rouard, Caroline
AU  - Rouard C
AD  - AP-HP, Hopitaux Universitaires Paris Sud, Hopital Antoine Beclere, Service de
      Bacteriologie-Hygiene, Clamart, France.
AD  - EA4043, Bacteries Pathogenes et Sante, Faculte de Pharmacie, Universite Paris
      Sud, Chatenay-Malabry, France.
FAU - Garnier, Fabien
AU  - Garnier F
AD  - CHU Limoges, Laboratoire de Bacteriologie-Virologie-Hygiene, Limoges, France.
AD  - INSERM, RESINFIT, U 1092, Universite Limoges, Limoges, France.
FAU - Leraut, Jeremy
AU  - Leraut J
AD  - AP-HP, Hopitaux Universitaires Paris Sud, Hopital Antoine Beclere, Service de
      Bacteriologie-Hygiene, Clamart, France.
FAU - Lepainteur, Margaux
AU  - Lepainteur M
AD  - AP-HP, Hopitaux Universitaires Paris Sud, Hopital Antoine Beclere, Service de
      Bacteriologie-Hygiene, Clamart, France.
FAU - Rahajamananav, Lalaina
AU  - Rahajamananav L
AD  - AP-HP, Hopitaux Universitaires Paris Sud, Hopital Antoine Beclere, Service de
      Bacteriologie-Hygiene, Clamart, France.
FAU - Languepin, Jeanne
AU  - Languepin J
AD  - Service de Pediatrie, Centre de Ressource et de Competence de la Mucoviscidose du
      Limousin, Hopital Mere Enfant, Limoges, France.
FAU - Ploy, Marie-Cecile
AU  - Ploy MC
AD  - CHU Limoges, Laboratoire de Bacteriologie-Virologie-Hygiene, Limoges, France.
AD  - INSERM, RESINFIT, U 1092, Universite Limoges, Limoges, France.
FAU - Bourgeois-Nicolaos, Nadege
AU  - Bourgeois-Nicolaos N
AD  - AP-HP, Hopitaux Universitaires Paris Sud, Hopital Antoine Beclere, Service de
      Bacteriologie-Hygiene, Clamart, France.
AD  - EA4043, Bacteries Pathogenes et Sante, Faculte de Pharmacie, Universite Paris
      Sud, Chatenay-Malabry, France.
FAU - Doucet-Populaire, Florence
AU  - Doucet-Populaire F
AD  - AP-HP, Hopitaux Universitaires Paris Sud, Hopital Antoine Beclere, Service de
      Bacteriologie-Hygiene, Clamart, France florence.doucet-populaire@aphp.fr.
AD  - EA4043, Bacteries Pathogenes et Sante, Faculte de Pharmacie, Universite Paris
      Sud, Chatenay-Malabry, France.
LA  - eng
PT  - Journal Article
DEP - 20181126
PL  - United States
TA  - Antimicrob Agents Chemother
JT  - Antimicrobial agents and chemotherapy
JID - 0315061
PMC - PMC6256785
OTO - NOTNLM
OT  - 23S rRNA
OT  - MRSA
OT  - Staphylococcus aureus
OT  - WGS
OT  - cystic fibrosis
OT  - linezolid
OT  - oxazolidinones
OT  - ribosomal resistance
EDAT- 2018/10/03 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/10/03 06:00
PMCR- 2019/05/26 00:00
PHST- 2018/04/14 00:00 [received]
PHST- 2018/09/22 00:00 [accepted]
PHST- 2019/05/26 00:00 [pmc-release]
PHST- 2018/10/03 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/10/03 06:00 [entrez]
AID - AAC.00720-18 [pii]
AID - 10.1128/AAC.00720-18 [doi]
PST - epublish
SO  - Antimicrob Agents Chemother. 2018 Nov 26;62(12). pii: AAC.00720-18. doi:
      10.1128/AAC.00720-18. Print 2018 Dec.