PMID- 30161236
OWN - NLM
STAT- MEDLINE
DCOM- 20190212
LR  - 20190215
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 8
DP  - 2018
TI  - Differences in the viral genome between HPV-positive cervical and oropharyngeal
      cancer.
PG  - e0203403
LID - 10.1371/journal.pone.0203403 [doi]
AB  - Human papillomavirus (HPV)-driven oropharyngeal cancer incidence in the United
      States has steadily increased in the past decades and has now become the most
      frequently diagnosed HPV-associated cancer type, surpassing cervical cancer.
      Variations in the HPV genome correlate with tumorigenic risk, and the
      distribution of genetic variants is extensively studied in cervical cancer, but
      very little is known about new mutations or the distribution of HPV types and
      variants in oropharyngeal cancer. Here we present an archival tissue cohort study
      that compares genomic characteristics of HPV associated with cervical versus
      oropharyngeal tumors using DNA sequence analysis. We found HPV16 to be more
      prevalent in oropharyngeal samples than in cervical samples (91.2% versus 52.9%),
      while HPV18 (1.5% versus 18.2%) and HPV45 (0.7% versus 9.9%) were much less
      prevalent. Differences between cervix and oropharynx in HPV16 variants
      distribution were more subtle, but the combined European + Asian (EUR+AS) variant
      group was more prevalent (90.2% versus 71.4%), while the American Asian 1 +
      American Asian 2 (AA1+AA2) variant group was much less prevalent (4.4% versus
      22.5%) in oropharyngeal cancers. HPV prevalence in oropharyngeal cancers showed
      an increasing trend from 60% in 2003 to 80% in 2016. We also identified over nine
      times more nonsynonymous mutations in the HPV E6 gene amplified from
      oropharyngeal samples, but for E7 the difference in mutation rates between the
      two anatomical locations was not significant. Overall, we showed that HPV genome 
      in oropharyngeal cancer presents important differences when compared to cervical 
      cancer and this may explain the distinct pathomechanisms and susceptibility to
      treatment of HPV-associated oropharyngeal cancer.
FAU - LeConte, Bailey A
AU  - LeConte BA
AD  - Department of Otolaryngology, The University of Texas Medical Branch at
      Galveston, Galveston, Texas, United States of America.
FAU - Szaniszlo, Peter
AU  - Szaniszlo P
AUID- ORCID: 0000-0002-3565-0957
AD  - Department of Otolaryngology, The University of Texas Medical Branch at
      Galveston, Galveston, Texas, United States of America.
FAU - Fennewald, Susan M
AU  - Fennewald SM
AD  - Department of Otolaryngology, The University of Texas Medical Branch at
      Galveston, Galveston, Texas, United States of America.
FAU - Lou, Dianne I
AU  - Lou DI
AD  - Department of Otolaryngology, The University of Texas Medical Branch at
      Galveston, Galveston, Texas, United States of America.
FAU - Qiu, Suimin
AU  - Qiu S
AD  - Department of Pathology, The University of Texas Medical Branch at Galveston,
      Galveston, Texas, United States of America.
FAU - Chen, Nai-Wei
AU  - Chen NW
AUID- ORCID: 0000-0002-7969-8805
AD  - Department of Preventive Medicine & Community Health, The University of Texas
      Medical Branch at Galveston, Galveston, Texas, United States of America.
FAU - Lee, John H
AU  - Lee JH
AD  - Department of Adult Medical Affairs, Chan Soon-Shiong Institute of Molecular
      Medicine, Culver City, California, United States of America.
FAU - Resto, Vicente A
AU  - Resto VA
AD  - Department of Otolaryngology, The University of Texas Medical Branch at
      Galveston, Galveston, Texas, United States of America.
LA  - eng
GR  - K08 CA132988/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180830
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Viral)
SB  - IM
MH  - DNA, Viral/genetics
MH  - Female
MH  - Genome, Viral/*genetics
MH  - Human papillomavirus 16/genetics
MH  - Humans
MH  - Oropharyngeal Neoplasms/etiology/*virology
MH  - Papillomaviridae/*genetics
MH  - Papillomavirus Infections/*complications/virology
MH  - Sequence Analysis, DNA
MH  - Uterine Cervical Neoplasms/etiology/*virology
PMC - PMC6117069
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/08/31 06:00
MHDA- 2019/02/13 06:00
CRDT- 2018/08/31 06:00
PHST- 2018/05/09 00:00 [received]
PHST- 2018/08/20 00:00 [accepted]
PHST- 2018/08/31 06:00 [entrez]
PHST- 2018/08/31 06:00 [pubmed]
PHST- 2019/02/13 06:00 [medline]
AID - 10.1371/journal.pone.0203403 [doi]
AID - PONE-D-18-14010 [pii]
PST - epublish
SO  - PLoS One. 2018 Aug 30;13(8):e0203403. doi: 10.1371/journal.pone.0203403.
      eCollection 2018.