PMID- 30153259
DCOM- 20190204
LR  - 20190215
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 8
DP  - 2018
TI  - Mycoplasma genitalium in the Far North Queensland backpacker population: An
      observational study of prevalence and azithromycin resistance.
PG  - e0202428
LID - 10.1371/journal.pone.0202428 [doi]
AB  - BACKGROUND: Mycoplasma genitalium is a sexually transmitted infection (STI), and 
      a common cause of non-gonococcal urethritis (NGU). There is concern regarding the
      rise in prevalence of M. genitalium and rates of resistance to macrolide
      antibiotics. International backpackers represent a unique population that may be 
      at an increased risk of STIs. The purpose of this study was to determine the
      prevalence of M. genitalium and antibiotic resistance in international
      backpackers. METHODS: First void urine samples were obtained utilising
      opportunistic sampling from 294 non-treatment-seeking international backpackers
      at a variety of hostels in Cairns, Queensland Australia. Participants also
      answered a fixed-answer survey regarding sociodemographic characteristics and
      sexual risk behaviours. Samples were tested for M. genitalium, Chlamydia
      trachomatis and Neisseria gonorrhoeae using polymerase chain reaction (PCR).
      Samples positive for M. genitalium were investigated for macrolide
      resistance-associated mutations in the 23S rRNA genome at positions A2058G,
      A2058C, A2058T, A2059G and A2059C (Escherichia coli numbering). RESULTS: Of the
      294 samples, 23 failed the internal control. The prevalence of M. genitalium was 
      1.8% (5/271, 95% confidence interval [CI] +/- 1.58), C. trachomatis was 4.1%
      (11/271, 95% CI +/- 2.36) and N. gonorrhoeae was not detected. Macrolide
      resistance-associated mutations were identified in 40% (2/5) of M.
      genitalium-positive samples. M. genitalium infection was associated with
      reporting symptoms (odds ratio [OR] 14.36, 95% CI 2.17-94.94, p < 0.05).
      CONCLUSIONS: M. genitalium and C. trachomatis are relatively common amongst
      non-treatment seeking international backpackers, but may not differ from
      Australian population prevalence. This article provides evidence to further
      support the increased utilisation of M. genitalium PCR in the diagnosis of NGU,
      and for macrolide resistance testing for all identified M. genitalium infections.
FAU - Trevis, Thomas
AU  - Trevis T
AUID- ORCID: 0000-0002-4823-8187
AD  - James Cook University Clinical School, Cairns Hospital, Cairns, Queensland,
FAU - Gosse, Marianne
AU  - Gosse M
AD  - Norwegian University of Science and Technology, Faculty of Medicine, Trondheim,
FAU - Santarossa, Nicola
AU  - Santarossa N
AD  - James Cook University Clinical School, Cairns Hospital, Cairns, Queensland,
FAU - Tabrizi, Sepehr
AU  - Tabrizi S
AD  - Department of Obstetrics and Gynaecology, University of Melbourne, Victoria,
FAU - Russell, Darren
AU  - Russell D
AD  - Cairns Sexual Health Service, Cairns North, Queensland, Australia.
FAU - McBride, William John
AU  - McBride WJ
AD  - James Cook University Clinical School, Cairns Hospital, Cairns, Queensland,
LA  - eng
SI  - Dryad/10.5061/dryad.fb45vd1
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180828
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 83905-01-5 (Azithromycin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Azithromycin
MH  - Cross-Sectional Studies
MH  - *Drug Resistance, Bacterial
MH  - Female
MH  - Humans
MH  - Male
MH  - Mycoplasma Infections/drug therapy/epidemiology/*microbiology
MH  - Mycoplasma genitalium/*isolation & purification
MH  - Prevalence
MH  - Queensland/epidemiology
MH  - Sexually Transmitted Diseases, Bacterial/drug therapy/epidemiology/*microbiology
PMC - PMC6112622
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/08/29 06:00
MHDA- 2019/02/05 06:00
CRDT- 2018/08/29 06:00
PHST- 2018/05/19 00:00 [received]
PHST- 2018/08/02 00:00 [accepted]
PHST- 2018/08/29 06:00 [entrez]
PHST- 2018/08/29 06:00 [pubmed]
PHST- 2019/02/05 06:00 [medline]
AID - 10.1371/journal.pone.0202428 [doi]
AID - PONE-D-18-15021 [pii]
PST - epublish
SO  - PLoS One. 2018 Aug 28;13(8):e0202428. doi: 10.1371/journal.pone.0202428.
      eCollection 2018.