PMID- 30074476
OWN - NLM
STAT- MEDLINE
DCOM- 20180911
LR  - 20180911
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 67
IP  - 9
DP  - 2018 Sep
TI  - Mycoplasma pneumoniae DNA detection and specific antibody class response in
      patients from two tertiary care hospitals in tropical Sri Lanka.
PG  - 1232-1242
LID - 10.1099/jmm.0.000813 [doi]
AB  - PURPOSE: Respiratory tract infections are a major cause of global morbidity and
      mortality. Pneumonia is the ninth leading cause of mortality in Sri Lanka.
      Atypical pathogens cause about one-fifth of community-acquired pneumonia, while
      Mycoplasma pneumoniae accounts for about 50 %. This study aimed to determine the 
      seroprevalence of M. pneumoniae respiratory tract infections in Sri Lanka while
      attempting to understand the relationships between the serology and PCR.
      METHODOLOGY: Paired sera from 418 adult patients (pneumonia, n=97; bronchitis,
      n=183; pharyngitis, n=138) and 87 healthy controls were studied. IgM, IgG and IgA
      antibodies were tested by M. pneumoniae enzyme-linked immunosorbent assay
      (ELISA). Positive IgM and or IgG seroconversion was considered to be
      seropositive. M. pneumoniae DNA were tested by PCR in age and gender-matched
      seropositives and seronegatives. RESULTS: M. pneumoniae IgG was in 14.4 %
      (14/97), 6.0 % (11/183) and 1.5 % (2/138) of pneumonia, bronchitis and
      pharyngitis patients, respectively, whilst IgM was in 6.2 % (6/97), 1.1 % (2/183)
      and 0 % (0/138), respectively. Amongst the pneumonia seropositives, 64.7 %
      (11/17) showed IgG alone, 17.5 % (3/17) showed IgM alone and 17.5 % (3/17) showed
      IgM and IgG. Amongst the bronchitis seropositives, 84.6 % (11/13) had IgG alone
      and 15.4 % (2/13) had IgM alone. In the pharyngitis seropositives, only IgG was
      detected 100 % (2/2). M. pneumoniae DNA was in 52.2 % (12/23) of seropositives
      and 15.4 % (4/26) of seronegatives. In pneumonia or bronchitis patients, specific
      DNA was in 77.8 % (7/10) and 50 % (6/12) of patients, respectively. M. pneumoniae
      DNA was not found in pharyngitis patients. Of the seropositive PCR-negative
      pneumonia patients, 66.7 % (2/3) showed IgG alone and 33.3 % (1/3)showed IgM
      alone. In bronchitis patients, 83.3 % (5/6) showed IgG alone and 16.7 % (1/6)
      showed IgM alone. Of the seronegative PCR-positive patients, 16.7 % (2/12) had
      pneumonia and 18.2 % (2/11) had bronchitis. CONCLUSION: The serological evidence 
      for M. pneumoniae infection in Sri Lanka comprised the following prevalences:
      17.5 % (17/97), 7.1 % (13/183) and 1.4 % (2/138) in adults with pneumonia,
      bronchitis or pharyngitis, respectively. M. pneumoniae DNA was in 52.2 % (12/23) 
      of seropositives and 15.4 % (4/26) of seronegatives. IgG was predominant in PCR
      positives and negatives.
FAU - Wijesooriya, Lakmini Inoka
AU  - Wijesooriya LI
AD  - 1Department of Medical Microbiology, Faculty of Medicine, University of Kelaniya,
      Sri Lanka.
FAU - Kok, Tuckweng
AU  - Kok T
AD  - 2Department of Microbiology and Infectious Diseases, School of Biological
      Sciences, University of Adelaide, Institute of Medical and Veterinary Science
      (IMVS), Adelaide, SA 5005, Australia.
FAU - Perera, Jennifer
AU  - Perera J
AD  - 3Department of Microbiology, Faculty of Medicine, University of Colombo, Sri
      Lanka.
FAU - Tilakarathne, Y
AU  - Tilakarathne Y
AD  - 4Department of Medicine, Faculty of Medicine, University of Kelaniya, Sri Lanka.
FAU - Sunil-Chandra, N P
AU  - Sunil-Chandra NP
AD  - 1Department of Medical Microbiology, Faculty of Medicine, University of Kelaniya,
      Sri Lanka.
LA  - eng
PT  - Journal Article
DEP - 20180803
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antibodies, Bacterial/blood
MH  - DNA, Bacterial/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin G/blood
MH  - Immunoglobulin M/blood
MH  - Male
MH  - Middle Aged
MH  - Mycoplasma pneumoniae/classification/genetics/immunology/*isolation &
      purification
MH  - Pneumonia, Mycoplasma/blood/epidemiology/*microbiology
MH  - Seroepidemiologic Studies
MH  - Sri Lanka/epidemiology
MH  - Tertiary Care Centers
MH  - Young Adult
OTO - NOTNLM
OT  - DNA detection
OT  - Mycoplasma pneumoniae
OT  - serology
EDAT- 2018/08/04 06:00
MHDA- 2018/09/12 06:00
CRDT- 2018/08/04 06:00
PHST- 2018/08/04 06:00 [pubmed]
PHST- 2018/09/12 06:00 [medline]
PHST- 2018/08/04 06:00 [entrez]
AID - 10.1099/jmm.0.000813 [doi]
PST - ppublish
SO  - J Med Microbiol. 2018 Sep;67(9):1232-1242. doi: 10.1099/jmm.0.000813. Epub 2018
      Aug 3.