PMID- 30025137
OWN - NLM
STAT- MEDLINE
DCOM- 20190304
LR  - 20190304
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 59
IP  - 7
DP  - 2018 Jun 1
TI  - Effects of Corneal Hydration on Brillouin Microscopy In Vivo.
PG  - 3020-3027
LID - 10.1167/iovs.18-24228 [doi]
AB  - Purpose: To investigate how corneal hydration affects the Brillouin frequency of 
      corneal stroma. Methods: From a simple analytical model considering the volume
      fraction of water in corneal stroma, we derived the dependence of Brillouin
      frequency on hydration and hydration-induced corneal thickness variation. The
      Brillouin frequencies of fresh ex vivo porcine corneas were measured as their
      hydration was varied in dextran solution and water. Healthy volunteers (8 eyes)
      were scanned in vivo repeatedly over the course of 9 hours, and the diurnal
      variations of Brillouin frequency and central corneal thickness (CCT) were
      measured. Results: The measured dependence of Brillouin frequency on hydration,
      both ex vivo and in vivo, agreed well with the theoretical prediction. The
      Brillouin frequencies of human corneas scanned immediately after waking were on
      average approximately 25 MHz lower than their daytime average values. For
      stabilized corneas, the typical variation of Brillouin frequency was +/- 7.2 MHz.
      With respect to CCT increase or swelling, the Brillouin frequency decreased with 
      a slope of -1.06 MHz/mum in vivo. Conclusions: The ex vivo and in vivo data agree
      with our theoretical model and support that the effect of corneal hydration on
      Brillouin frequency comes predominantly from the dependence of the tissue
      compressibility on the water. Corneal hydration correlates negatively with the
      Brillouin frequency. During daytime activities, the influence of physiological
      hydration changes in human corneas is < +/- 10 MHz. The sensitivity to hydration 
      may potentially be useful in detecting abnormal hydration change in patients with
      endothelial disorders.
FAU - Shao, Peng
AU  - Shao P
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard
      Medical School, Boston, Massachusetts, United States.
FAU - Seiler, Theo G
AU  - Seiler TG
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard
      Medical School, Boston, Massachusetts, United States.
AD  - Institut fur Refraktive und Ophthalmo-Chirurgie (IROC), Zurich, Switzerland.
AD  - Universitatsklinik fur Augenheilkunde, Inselspital, Bern, Switzerland.
FAU - Eltony, Amira M
AU  - Eltony AM
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard
      Medical School, Boston, Massachusetts, United States.
FAU - Ramier, Antoine
AU  - Ramier A
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard
      Medical School, Boston, Massachusetts, United States.
AD  - Harvard-MIT Health Sciences and Technology, Massachusetts Institute of
      Technology, Cambridge, Massachusetts, United States.
FAU - Kwok, Sheldon J J
AU  - Kwok SJJ
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard
      Medical School, Boston, Massachusetts, United States.
AD  - Harvard-MIT Health Sciences and Technology, Massachusetts Institute of
      Technology, Cambridge, Massachusetts, United States.
FAU - Scarcelli, Giuliano
AU  - Scarcelli G
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard
      Medical School, Boston, Massachusetts, United States.
AD  - Fischell Department of Bioengineering, University of Maryland, College Park,
      Maryland, United States.
FAU - Ii, Roberto Pineda
AU  - Ii RP
AD  - Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States.
FAU - Yun, Seok-Hyun
AU  - Yun SH
AD  - Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard
      Medical School, Boston, Massachusetts, United States.
AD  - Harvard-MIT Health Sciences and Technology, Massachusetts Institute of
      Technology, Cambridge, Massachusetts, United States.
AD  - Department of Dermatology, Harvard Medical School, Boston, Massachusetts, United 
      States.
LA  - eng
GR  - UL1 RR025758/RR/NCRR NIH HHS/United States
GR  - R01 EY025454/EY/NEI NIH HHS/United States
GR  - P41 EB015903/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Adult
MH  - Animals
MH  - Body Water/*metabolism
MH  - Cornea/*metabolism
MH  - Corneal Pachymetry
MH  - Elastic Modulus
MH  - Elasticity/physiology
MH  - Healthy Volunteers
MH  - Humans
MH  - *Intravital Microscopy
MH  - Male
MH  - *Organism Hydration Status
MH  - Swine
PMC - PMC5995485
EDAT- 2018/07/20 06:00
MHDA- 2019/03/05 06:00
CRDT- 2018/07/20 06:00
PHST- 2018/07/20 06:00 [entrez]
PHST- 2018/07/20 06:00 [pubmed]
PHST- 2019/03/05 06:00 [medline]
AID - 2684942 [pii]
AID - 10.1167/iovs.18-24228 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2018 Jun 1;59(7):3020-3027. doi:
      10.1167/iovs.18-24228.