PMID- 30024965
OWN - NLM
STAT- MEDLINE
DCOM- 20190117
LR  - 20190117
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 7
DP  - 2018
TI  - Multi-locus sequence typing of Treponema pallidum subsp. pallidum present in
      clinical samples from France: Infecting treponemes are genetically diverse and
      belong to 18 allelic profiles.
PG  - e0201068
LID - 10.1371/journal.pone.0201068 [doi]
AB  - Treponema pallidum subsp. pallidum, the causative agent of sexually transmitted
      syphilis, detected in clinical samples from France, was subjected to molecular
      typing using the recently developed Multilocus Sequence Typing system. The
      samples (n = 133) used in this study were collected from 2010-2016 from patients 
      with diagnosed primary or secondary syphilis attending outpatient centers or
      hospitals in several locations in France. Altogether, 18 different allelic
      profiles were found among the fully typed samples (n = 112). There were five
      allelic variants identified for TP0136, 12 for TP0548, and eight for TP0705. Out 
      of the identified alleles, one, seven, and three novel alleles were identified in
      TP0136, TP0548, and TP0705, respectively. Partial allelic profiles were obtained 
      from 6 samples. The majority of samples (n = 110) belonged to the SS14-like
      cluster of TPA isolates while 7 clustered with Nichols-like isolates. Patients
      infected with Nichols-like samples were more often older (p = 0.041) and more
      often diagnosed with secondary syphilis (p = 0.033) compared to patients infected
      with SS14-like samples. In addition, macrolide resistance caused by the A2058G
      mutation was found to be associated with allelic profile 1.3.1 or with strains
      belonging to the 1.3.1 lineage (p<0.001). The genetic diversity among TPA strains
      infecting the European population was surprisingly high, which suggests that
      additional studies are needed to reveal the full genetic diversity of TPA
      pathogens infecting humans.
FAU - Pospisilova, Petra
AU  - Pospisilova P
AD  - Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech
      Republic.
FAU - Grange, Philippe Alain
AU  - Grange PA
AD  - Institut Cochin U1016 Equipe Batteux, Laboratoire de Dermatologie-CNR Syphilis,
      Faculte de Medecine, Universite Sorbonne Paris Descartes, Paris, France.
FAU - Grillova, Linda
AU  - Grillova L
AD  - Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech
      Republic.
FAU - Mikalova, Lenka
AU  - Mikalova L
AD  - Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech
      Republic.
FAU - Martinet, Pervenche
AU  - Martinet P
AD  - Service Prevention Sante Conseil Departemental des Bouches du Rhone, Marseille,
      France.
FAU - Janier, Michel
AU  - Janier M
AD  - Centre des MST, Hopital Saint-Louis, AP-HP, Paris, France.
FAU - Vermersch, Annie
AU  - Vermersch A
AD  - Service de Dermatologie, Hopital Jean Bernard, Valenciennes, France.
FAU - Benhaddou, Nadjet
AU  - Benhaddou N
AD  - Institut Cochin U1016 Equipe Batteux, Laboratoire de Dermatologie-CNR Syphilis,
      Faculte de Medecine, Universite Sorbonne Paris Descartes, Paris, France.
AD  - Service de Bacteriologie, Groupe Hospitalier Paris Centre Cochin-Hotel
      Dieu-Broca, Paris, France.
FAU - Del Giudice, Pascal
AU  - Del Giudice P
AD  - Service de Dermatologie-Infectiologie, Centre Hospitalier Inter regional, Frejus,
      France.
FAU - Alcaraz, Isabelle
AU  - Alcaraz I
AD  - Service Universitaire des Maladies Infectieuses et du Voyageur, Hopital Dron,
      Tourcoing, France.
FAU - Truchetet, Francois
AU  - Truchetet F
AD  - Service de Dermatologie, Hopital Bel Air, Metz-Thionville, France.
FAU - Dupin, Nicolas
AU  - Dupin N
AUID- ORCID: 0000-0002-6237-4951
AD  - Institut Cochin U1016 Equipe Batteux, Laboratoire de Dermatologie-CNR Syphilis,
      Faculte de Medecine, Universite Sorbonne Paris Descartes, Paris, France.
AD  - Service de Dermatologie-Venereologie, Hopital Cochin-Pavillon Tarnier, AP-HP,
      Paris, France.
FAU - Smajs, David
AU  - Smajs D
AUID- ORCID: 0000-0002-4176-3464
AD  - Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech
      Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180719
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Bacterial Typing Techniques
MH  - Biodiversity
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - France/epidemiology
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Middle Aged
MH  - Multilocus Sequence Typing
MH  - Syphilis/*epidemiology/*microbiology
MH  - Treponema pallidum/*genetics/isolation & purification
MH  - Young Adult
PMC - PMC6053231
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/07/20 06:00
MHDA- 2019/01/18 06:00
CRDT- 2018/07/20 06:00
PHST- 2018/04/12 00:00 [received]
PHST- 2018/07/07 00:00 [accepted]
PHST- 2018/07/20 06:00 [entrez]
PHST- 2018/07/20 06:00 [pubmed]
PHST- 2019/01/18 06:00 [medline]
AID - 10.1371/journal.pone.0201068 [doi]
AID - PONE-D-18-11051 [pii]
PST - epublish
SO  - PLoS One. 2018 Jul 19;13(7):e0201068. doi: 10.1371/journal.pone.0201068.
      eCollection 2018.