PMID- 29969089
OWN - NLM
STAT- MEDLINE
DCOM- 20180911
LR  - 20180911
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 67
IP  - 9
DP  - 2018 Sep
TI  - Distribution of genes encoding virulence factors and multilocus variable-number
      tandem-repeat analysis (MLVA) of entero-aggregative Escherichia coli (EAEC)
      isolated in Iran from patients with diarrhoea.
PG  - 1334-1339
LID - 10.1099/jmm.0.000786 [doi]
AB  - PURPOSE: Entero-aggregative Escherichia coli (EAEC) is one of the main causes of 
      diarrhoea worldwide. Several virulence factors have been identified in EAEC. This
      study was conducted to investigate the distribution of virulence factor genes in 
      EAEC strains isolated in Iran from children with diarrhoea, as well as the
      genetic similarity of these isolates. METHODOLOGY: A total of 37 EAEC isolates
      were tested for the presence of 11 virulence genes by PCR, and the genetic
      relatedness of these strains was further determined by multilocus variable-number
      tandem-repeat analysis (MLVA). RESULTS: All EAEC isolates were typical EAEC. pic,
      set1A and set1B were the most prevalent genes, detected in 54.1 % of the
      isolates, followed by sat (43.2 %), astA (32.4 %), pet (24.3 %), agg4A (24.3 %), 
      sepA (18.9 %), agg3A (13.5 %), sigA (8.1 %), aggA (8.1 %) and aafA (5.4 %). Using
      MLVA, the 37 isolates were divided into 32 types and classified into five clonal 
      complexes. CONCLUSION: This study showed that EAEC is a heterogeneous group of E.
      coli possessing a broad range of virulence factors. There was no notable
      association between MLVA patterns and virulence profiles.
FAU - Hosseini-Nave, Hossein
AU  - Hosseini-Nave H
AD  - 1Department of Microbiology and Virology, School of Medicine, Kerman University
      of Medical Sciences, Kerman, Iran.
FAU - Samareh Salavati, Maryam
AU  - Samareh Salavati M
AD  - 2Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.
FAU - Lashkari, Mahla
AU  - Lashkari M
AD  - 2Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.
FAU - Ravari, Mehrnaze Alsadat
AU  - Ravari MA
AD  - 2Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.
FAU - Hashemizadeh, Zahra
AU  - Hashemizadeh Z
AD  - 3Bacteriology and Virology, School of Medicine, Shiraz University of Medical
      Sciences, Shiraz, IR, Iran.
FAU - Seyedi Marghaki, Farhad
AU  - Seyedi Marghaki F
AD  - 1Department of Microbiology and Virology, School of Medicine, Kerman University
      of Medical Sciences, Kerman, Iran.
FAU - Mohseni, Fahimeh
AU  - Mohseni F
AD  - 2Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.
LA  - eng
PT  - Journal Article
DEP - 20180703
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Diarrhea/*microbiology
MH  - Escherichia coli/classification/genetics/*isolation & purification
MH  - Escherichia coli Infections/*microbiology
MH  - Escherichia coli Proteins/genetics/metabolism
MH  - Female
MH  - Humans
MH  - Infant
MH  - Iran
MH  - Male
MH  - Minisatellite Repeats
MH  - Phylogeny
MH  - Virulence Factors/*genetics/metabolism
OTO - NOTNLM
OT  - MLVA
OT  - enteroaggregative Escherichia coli
OT  - virulence factors
EDAT- 2018/07/04 06:00
MHDA- 2018/09/12 06:00
CRDT- 2018/07/04 06:00
PHST- 2018/07/04 06:00 [pubmed]
PHST- 2018/09/12 06:00 [medline]
PHST- 2018/07/04 06:00 [entrez]
AID - 10.1099/jmm.0.000786 [doi]
PST - ppublish
SO  - J Med Microbiol. 2018 Sep;67(9):1334-1339. doi: 10.1099/jmm.0.000786. Epub 2018
      Jul 3.