PMID- 29698402
OWN - NLM
STAT- MEDLINE
DCOM- 20180718
LR  - 20181114
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 4
DP  - 2018
TI  - A new method for evaluating tumor-infiltrating lymphocytes (TILs) in colorectal
      cancer using hematoxylin and eosin (H-E)-stained tumor sections.
PG  - e0192744
LID - 10.1371/journal.pone.0192744 [doi]
AB  - PURPOSE: Numerous reports indicate that tumor-infiltrating lymphocytes (TILs) are
      a prognostic factor in various cancers and that they must be good biomarkers.
      However, the methods of evaluating TILs differ in each study; thus, there is not 
      yet a standardized methodology for evaluating TILs. The purpose of this study is 
      to evaluate the prognostic significance of tumor-infiltrating lymphocytes (TILs) 
      in patients with colorectal cancer (CRC) using the new method proposed by the
      International TILs Working Group in breast cancer and to standardize the method
      of evaluating TILs in CRC. METHODS: We retrospectively reviewed a database of 160
      patients with Stage II or III CRC. The density of TILs was assessed by measuring 
      the area occupied by mononuclear cells over the stromal area on hematoxylin and
      eosin (H-E)-stained sections. We set 42% as the cut-off percentage of the area
      occupied by TILs according to the receiver operating characteristic curve, and we
      classified patients into the high-TILs and the low-TILs groups. RESULTS: The
      rates of relapse-free survival (RFS) and overall survival (OS) in the high-TILs
      group were significantly higher than those in the low-TILs group. A multivariate 
      analysis showed that the density of TILs was independently associated with RFS
      and OS. Moreover, the density of TILs assessed by an observer was significantly
      associated with the density of TILs assessed by the automated imaging software
      program. CONCLUSIONS: The new method for evaluating TILs, which was recommended
      by the International TILs Working Group in breast cancer, might be a useful
      predictive factor in colorectal cancer patients.
FAU - Iseki, Yasuhito
AU  - Iseki Y
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Shibutani, Masatsune
AU  - Shibutani M
AUID- ORCID: 0000-0002-2164-7394
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Maeda, Kiyoshi
AU  - Maeda K
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Nagahara, Hisashi
AU  - Nagahara H
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Fukuoka, Tatsunari
AU  - Fukuoka T
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Matsutani, Shinji
AU  - Matsutani S
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Kashiwagi, Shinichiro
AU  - Kashiwagi S
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Tanaka, Hiroaki
AU  - Tanaka H
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Hirakawa, Kosei
AU  - Hirakawa K
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
FAU - Ohira, Masaichi
AU  - Ohira M
AD  - Department of Surgical Oncology, Osaka City University Graduate School of
      Medicine, Abeno-ku, Osaka, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180426
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Fluorescent Dyes)
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
RN  - YKM8PY2Z55 (Hematoxylin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Colorectal Neoplasms/*mortality/*pathology
MH  - Eosine Yellowish-(YS)
MH  - Female
MH  - Fluorescent Dyes
MH  - Hematoxylin
MH  - Humans
MH  - Image Processing, Computer-Assisted/methods
MH  - Lymphocytes, Tumor-Infiltrating/*pathology
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Staining and Labeling/*methods
PMC - PMC5919485
EDAT- 2018/04/27 06:00
MHDA- 2018/07/19 06:00
CRDT- 2018/04/27 06:00
PHST- 2017/10/02 00:00 [received]
PHST- 2018/01/30 00:00 [accepted]
PHST- 2018/04/27 06:00 [entrez]
PHST- 2018/04/27 06:00 [pubmed]
PHST- 2018/07/19 06:00 [medline]
AID - 10.1371/journal.pone.0192744 [doi]
AID - PONE-D-17-35580 [pii]
PST - epublish
SO  - PLoS One. 2018 Apr 26;13(4):e0192744. doi: 10.1371/journal.pone.0192744.
      eCollection 2018.