PMID- 29641543
OWN - NLM
STAT- MEDLINE
DCOM- 20180716
LR  - 20190329
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 4
DP  - 2018
TI  - Urogenital Chlamydia trachomatis multilocus sequence types and genovar
      distribution in chlamydia infected patients in a multi-ethnic region of Saratov, 
      Russia.
PG  - e0195386
LID - 10.1371/journal.pone.0195386 [doi]
AB  - BACKGROUND: This is the first report to characterize the prevalence and genovar
      distribution of genital chlamydial infections among random heterosexual patients 
      in the multi-ethnic Saratov Region, located in Southeast Russia. METHODS:
      Sixty-one clinical samples (cervical or urethral swabs) collected from a random
      cohort of 856 patients (7.1%) were C. trachomatis (CT) positive in commercial
      nucleic acid amplification tests (NAATs) and duplex TaqMan PCRs. RESULTS:
      Sequence analysis of the VDII region of the ompA gene revealed seven genovars of 
      C. trachomatis in PCR-positive patients. The overall genovars were distributed as
      E (41.9%), G (21.6%), F (13.5%), K (9.5%), D (6.8%), J (4.1%), and H (2.7%).
      CT-positive samples were from males (n = 12, 19.7%), females (n = 42, 68.8%), and
      anonymous (n = 7, 11.5%) patients, with an age range of 19 to 45 years (average
      26.4), including 12 different ethnic groups representative of this region. Most
      patients were infected with a single genovar (82%), while 18% were co-infected
      with either two or three genovars. The 1156 bp-fragment of the ompA gene was
      sequenced in 46 samples to determine single nucleotide polymorphisms (SNP) among 
      isolates. SNP-based subtyping and phylogenetic reconstruction revealed the
      presence of 13 variants of the ompA gene, such as E (E1, E2, E6), G (G1, G2, G3, 
      G5), F1, K, D (D1, Da2), J1, and H2. Differing genovar distribution was
      identified among urban (E>G>F) and rural (E>K) populations, and in Slavic (E>G>D)
      and non-Slavic (E>G>K) ethnic groups. Multilocus sequence typing (MLST)
      determined five sequences types (STs), such as ST4 (56%, 95% confidence interval,
      CI, 70.0 to 41.3), ST6 (10%, 95% CI 21.8 to 3.3), ST9 (22%, 95% CI 35.9 to 11.5),
      ST10 (2%, 95% CI 10.7 to 0.05) and ST38 (10%, 95% CI 21.8 to 3.3). Thus, the most
      common STs were ST4 and ST9. CONCLUSION: C. trachomatis is a significant cause of
      morbidity among random heterosexual patients with genital chlamydial infections
      in the Saratov Region. Further studies should extend this investigation by
      describing trends in a larger population, both inside and outside of the Saratov 
      Region to clarify some aspects for the actual application of C. trachomatis
      genotype analysis for disease control.
FAU - Feodorova, Valentina A
AU  - Feodorova VA
AUID- ORCID: 0000-0002-3827-407X
AD  - Laboratory for Molecular Biology of Chlamydia, Federal Research Center for
      Virology and Microbiology (FRCViM), Pokrov, Vladimir region, Russia.
AD  - Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center
      for Virology and Microbiology (FRCViM), Branch in Saratov, Saratov, Russia.
AD  - Department for Microbiology, Biotechnology and Chemistry, Saratov State Agrarian 
      University (SSAU), Saratov, Russia.
FAU - Konnova, Svetlana S
AU  - Konnova SS
AD  - Laboratory for Molecular Biology of Chlamydia, Federal Research Center for
      Virology and Microbiology (FRCViM), Pokrov, Vladimir region, Russia.
FAU - Saltykov, Yury V
AU  - Saltykov YV
AD  - Laboratory for Molecular Biology of Chlamydia, Federal Research Center for
      Virology and Microbiology (FRCViM), Pokrov, Vladimir region, Russia.
AD  - Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center
      for Virology and Microbiology (FRCViM), Branch in Saratov, Saratov, Russia.
AD  - Department for Microbiology, Biotechnology and Chemistry, Saratov State Agrarian 
      University (SSAU), Saratov, Russia.
FAU - Zaitsev, Sergey S
AU  - Zaitsev SS
AD  - Laboratory for Molecular Biology of Chlamydia, Federal Research Center for
      Virology and Microbiology (FRCViM), Pokrov, Vladimir region, Russia.
AD  - Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center
      for Virology and Microbiology (FRCViM), Branch in Saratov, Saratov, Russia.
FAU - Subbotina, Irina A
AU  - Subbotina IA
AD  - Laboratory for Molecular Biology of Chlamydia, Federal Research Center for
      Virology and Microbiology (FRCViM), Pokrov, Vladimir region, Russia.
AD  - Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center
      for Virology and Microbiology (FRCViM), Branch in Saratov, Saratov, Russia.
AD  - Department for Microbiology, Biotechnology and Chemistry, Saratov State Agrarian 
      University (SSAU), Saratov, Russia.
FAU - Polyanina, Tatiana I
AU  - Polyanina TI
AD  - Laboratory for Molecular Biology of Chlamydia, Federal Research Center for
      Virology and Microbiology (FRCViM), Pokrov, Vladimir region, Russia.
AD  - Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center
      for Virology and Microbiology (FRCViM), Branch in Saratov, Saratov, Russia.
FAU - Ulyanov, Sergey S
AU  - Ulyanov SS
AD  - Laboratory for Molecular Biology of Chlamydia, Federal Research Center for
      Virology and Microbiology (FRCViM), Pokrov, Vladimir region, Russia.
AD  - Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center
      for Virology and Microbiology (FRCViM), Branch in Saratov, Saratov, Russia.
AD  - Department for Medical Physics, Saratov State University (SSU), Saratov, Russia.
FAU - Lamers, Susanna L
AU  - Lamers SL
AD  - Bioinfoexperts LLC, Thibodaux, Los Angeles, United States of America.
FAU - Gaydos, Charlotte A
AU  - Gaydos CA
AD  - Division of Infectious Diseases, Johns Hopkins University School of Medicine
      (JHUScM), Baltimore, Maryland, United States of America.
FAU - Quinn, Thomas C
AU  - Quinn TC
AD  - Division of Infectious Diseases, Johns Hopkins University School of Medicine
      (JHUScM), Baltimore, Maryland, United States of America.
AD  - Division of Intramural Research, National Institute of Allergy and Infectious
      Diseases (NIAID), Baltimore, Maryland, United States of America.
FAU - Motin, Vladimir L
AU  - Motin VL
AD  - Department of Pathology, Department of Microbiology & Immunology, University of
      Texas Medical Branch (UTMB), Galveston, Texas, United States of America.
LA  - eng
GR  - U54 EB007958/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180411
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 149024-69-1 (OMPA outer membrane proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bacterial Outer Membrane Proteins/genetics
MH  - Chlamydia Infections/*epidemiology/ethnology
MH  - Chlamydia trachomatis/*classification/genetics/*physiology
MH  - *Ethnic Groups
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Multilocus Sequence Typing
MH  - Prevalence
MH  - Russia/epidemiology/ethnology
MH  - Urogenital System/*microbiology
MH  - Young Adult
PMC - PMC5895025
EDAT- 2018/04/12 06:00
MHDA- 2018/07/17 06:00
CRDT- 2018/04/12 06:00
PHST- 2017/09/30 00:00 [received]
PHST- 2018/03/21 00:00 [accepted]
PHST- 2018/04/12 06:00 [entrez]
PHST- 2018/04/12 06:00 [pubmed]
PHST- 2018/07/17 06:00 [medline]
AID - 10.1371/journal.pone.0195386 [doi]
AID - PONE-D-17-35368 [pii]
PST - epublish
SO  - PLoS One. 2018 Apr 11;13(4):e0195386. doi: 10.1371/journal.pone.0195386.
      eCollection 2018.