PMID- 29566085
OWN - NLM
STAT- MEDLINE
DCOM- 20180727
LR  - 20181114
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 3
DP  - 2018
TI  - Urine metabolome in women with Chlamydia trachomatis infection.
PG  - e0194827
LID - 10.1371/journal.pone.0194827 [doi]
AB  - The aim of this study was to characterize the urine metabolome of women with
      Chlamydia trachomatis (CT) uro-genital infection (n = 21), comparing it with a
      group of CT-negative subjects (n = 98). By means of a proton-based nuclear
      magnetic resonance (1H-NMR) spectroscopy, we detected and quantified the urine
      metabolites of a cohort of 119 pre-menopausal Caucasian women, attending a STI
      Outpatients Clinic in Italy. In case of a CT positive result, CT molecular
      genotyping was performed by omp1 gene semi-nested PCR followed by RFLP analysis. 
      We were able to identify several metabolites whose concentrations were
      significantly higher in the urine samples of CT-positive subjects, including
      sucrose, mannitol, pyruvate and lactate. In contrast, higher urinary levels of
      acetone represented the main feature of CT-negative women. These results were not
      influenced by the age of patients nor by the CT serovars (D, E, F, G, K)
      responsible of the urethral infections. Since the presence of sugars can increase
      the stability of chlamydial proteins, higher levels of sucrose and mannitol in
      the urethral lumen, related to a higher sugar consumption, could have favoured CT
      infection acquisition or could have been of aid for the bacterial viability.
      Peculiar dietary habits of the subjects enrolled, in term of type and amount of
      food consumed, could probably explain these findings. Lactate and pyruvate could 
      result from CT-induced immunopathology, as a product of the inflammatory
      microenvironment. Further studies are needed to understand the potential role of 
      these metabolites in the pathogenesis of CT infection, as well as their
      diagnostic/prognostic use.
FAU - Foschi, Claudio
AU  - Foschi C
AUID- ORCID: 0000-0001-7253-8799
AD  - Microbiology, DIMES, University of Bologna, Bologna, Italy.
FAU - Laghi, Luca
AU  - Laghi L
AD  - Centre of Foodomics, Department of Agro-Food Science and Technology, University
      of Bologna, Cesena, Italy.
FAU - D'Antuono, Antonietta
AU  - D'Antuono A
AD  - Dermatology, DIMES, University of Bologna, Bologna, Italy.
FAU - Gaspari, Valeria
AU  - Gaspari V
AD  - Dermatology, DIMES, University of Bologna, Bologna, Italy.
FAU - Zhu, Chenglin
AU  - Zhu C
AUID- ORCID: 0000-0003-3486-1936
AD  - Centre of Foodomics, Department of Agro-Food Science and Technology, University
      of Bologna, Cesena, Italy.
FAU - Dellarosa, Nicolo
AU  - Dellarosa N
AD  - Centre of Foodomics, Department of Agro-Food Science and Technology, University
      of Bologna, Cesena, Italy.
FAU - Salvo, Melissa
AU  - Salvo M
AD  - Microbiology, DIMES, University of Bologna, Bologna, Italy.
FAU - Marangoni, Antonella
AU  - Marangoni A
AD  - Microbiology, DIMES, University of Bologna, Bologna, Italy.
LA  - eng
PT  - Journal Article
DEP - 20180322
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adult
MH  - Ambulatory Care Facilities
MH  - Chlamydia Infections/microbiology/*urine
MH  - *Chlamydia trachomatis/genetics/metabolism
MH  - Cohort Studies
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Italy
MH  - *Metabolome
MH  - Metabolomics/methods
MH  - Polymerase Chain Reaction/methods
MH  - Polymorphism, Restriction Fragment Length
MH  - Urinalysis/*methods
MH  - Urinary Tract Infections/diagnosis/microbiology/*urine
MH  - Young Adult
PMC - PMC5864028
EDAT- 2018/03/23 06:00
MHDA- 2018/07/28 06:00
CRDT- 2018/03/23 06:00
PHST- 2018/01/26 00:00 [received]
PHST- 2018/03/09 00:00 [accepted]
PHST- 2018/03/23 06:00 [entrez]
PHST- 2018/03/23 06:00 [pubmed]
PHST- 2018/07/28 06:00 [medline]
AID - 10.1371/journal.pone.0194827 [doi]
AID - PONE-D-18-02774 [pii]
PST - epublish
SO  - PLoS One. 2018 Mar 22;13(3):e0194827. doi: 10.1371/journal.pone.0194827.
      eCollection 2018.