PMID- 29425282
OWN - NLM
STAT- MEDLINE
DCOM- 20190625
LR  - 20190625
IS  - 1460-2091 (Electronic)
IS  - 0305-7453 (Linking)
VI  - 73
IP  - 5
DP  - 2018 May 1
TI  - Presence and molecular characteristics of oxazolidinone resistance in
      staphylococci from household animals in rural China.
PG  - 1194-1200
LID - 10.1093/jac/dky009 [doi]
AB  - Objectives: To investigate the presence and molecular characteristics of
      oxazolidinone resistance genes cfr and optrA in staphylococci from household
      animals in rural China. Methods: Various samples were collected from household
      animals in 12 rural villages. Staphylococcal isolates showing florfenicol MICs
      >/=10 mg/L were identified and screened for the presence of cfr and/or optrA.
      PCR-positive isolates were characterized by antimicrobial susceptibility testing,
      S1 nuclease PFGE and Southern blotting. WGS data were analysed to identify the
      core-genome phylogenetic profile of each isolate as well as the genetic
      environment of cfr and/or optrA. Results: Nine optrA-positive (seven
      Staphylococcus sciuri and two Staphylococcus simulans) and 10 cfr-positive
      staphylococci were identified from eight and five villages, respectively. The
      gene optrA was chromosomally encoded in all nine isolates, whereas cfr was
      located on a plasmid in one S. sciuri and three Staphylococcus saprophyticus and 
      in the chromosomal DNA of single Staphylococcus cohnii and Staphylococcus lentus 
      isolates and two S. sciuri isolates. The remaining two cfr-carrying
      Staphylococcus haemolyticus isolates were indistinguishable by PFGE. Most optrA- 
      or cfr-carrying staphylococci also harboured phenicol, tetracycline and/or
      macrolide-lincosamide-streptogramin B resistance genes. Genetic environment
      analysis showed that, for the first time, optrA was associated with transposon
      Tn6261, while cfr was adjacent to both a tnp (transposase) gene and a Tn558
      transposon. Conclusions: The current study reveals for the first time the wide
      distribution of oxazolidinone resistance genes optrA and cfr in household animals
      in rural areas of China and is the first identification of optrA in S. simulans
      isolates.
FAU - Sun, Chengtao
AU  - Sun C
AD  - Beijing Advanced Innovation Centre for Food Nutrition and Human Health, College
      of Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Beijing Advanced Innovation Centre for Food Nutrition and Human Health, College
      of Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Ji, Xing
AU  - Ji X
AD  - Beijing Advanced Innovation Centre for Food Nutrition and Human Health, College
      of Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Fan, Run
AU  - Fan R
AD  - Beijing Advanced Innovation Centre for Food Nutrition and Human Health, College
      of Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Chen, Baoli
AU  - Chen B
AD  - Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, 
      Shandong Centre for Disease Control and Prevention, Jinan, Shandong, China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Beijing Advanced Innovation Centre for Food Nutrition and Human Health, College
      of Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Schwarz, Stefan
AU  - Schwarz S
AD  - Beijing Advanced Innovation Centre for Food Nutrition and Human Health, College
      of Veterinary Medicine, China Agricultural University, Beijing, China.
AD  - Institute of Microbiology and Epizootics, Centre for Infection Medicine,
      Department of Veterinary Medicine, Freie Universitat Berlin, Berlin, Germany.
FAU - Wu, Congming
AU  - Wu C
AD  - Beijing Advanced Innovation Centre for Food Nutrition and Human Health, College
      of Veterinary Medicine, China Agricultural University, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Oxazolidinones)
SB  - IM
MH  - Animals
MH  - *Animals, Domestic
MH  - Anti-Infective Agents/*pharmacology
MH  - Bacterial Proteins/genetics
MH  - Blotting, Southern
MH  - China
MH  - *Drug Resistance, Bacterial
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Genotype
MH  - Microbial Sensitivity Tests
MH  - Oxazolidinones/*pharmacology
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Staphylococcal Infections/microbiology/*veterinary
MH  - Staphylococcus/classification/genetics/*isolation & purification
MH  - Whole Genome Sequencing
EDAT- 2018/02/10 06:00
MHDA- 2019/06/27 06:00
CRDT- 2018/02/10 06:00
PHST- 2017/09/08 00:00 [received]
PHST- 2018/01/02 00:00 [accepted]
PHST- 2018/02/10 06:00 [pubmed]
PHST- 2019/06/27 06:00 [medline]
PHST- 2018/02/10 06:00 [entrez]
AID - 4841776 [pii]
AID - 10.1093/jac/dky009 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2018 May 1;73(5):1194-1200. doi: 10.1093/jac/dky009.