PMID- 29420609
OWN - NLM
STAT- MEDLINE
DCOM- 20180409
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 2
DP  - 2018
TI  - Construction and analysis for differentially expressed long non-coding RNAs and
      MicroRNAs mediated competing endogenous RNA network in colon cancer.
PG  - e0192494
LID - 10.1371/journal.pone.0192494 [doi]
AB  - Long non-coding RNA (lncRNA) has been confirmed to act as a key regulatory
      molecule in different types of cancers and play a significant role in tumors
      initiation and progression. LncRNA can be as acompeting endogenous RNA(ceRNA) to 
      regulate the expression of targeted genes by sponging miRNA. In the present
      study, we explore the functional roles and regulatory mechanisms of lncRNAs as
      ceRNAs in colon cancer and their potential implications for prognosis.The
      lncRNAs, miRNAs and mRNAs expression profiles of 341 colon cancer tissues and 27 
      non-tumor colon tissues were downloaded from The Cancer Genome Atlas (TCGA)
      database. Differential expression of RNAs was identified using the "DESeq"
      bioconductor package in R. PPI network of differentially expressed genes was
      constructed using the STRING database. Survival analysis was estimated based on
      Kaplan-Meier curve analysis. We used KOBAS 3.0 to analyze the KEGG pathway of
      DEGs. The dysregulated lncRNA-associated ceRNA network was constructed in colon
      cancer based on bioinformatics generated from miRanda, PicTar, TargetScan, miRDB 
      and miRcode. A total of 791 DElncRNAs and 200 DEmiRNAs were identified in colon
      cancer compared with matched normal tissues with thresholds of |log2foldChange
      (FC)| >3.0and adjusted P value<0.05.Twenty DElncRNAs were identified, may be
      related to tumorigenesis and/or progression of colon cancer. Nine out of 20
      dysregulated lncRNA were found to be significantly associated with overall
      survival (P value<0.05). Finally, we successfully constructed colon
      cancer-associated ceRNA network, including 9 colon cancer-specific lncRNAs, 13
      miRNAS and 70 mRNAs. In conclusion, our study will contribute to improve the
      understanding of ceRNA network regulatory mechanisms in colon cancer. These
      identified novel lncRNAs can be as candidate prognostic biomarkers or potential
      therapeutic targets.
FAU - Li, Fengxi
AU  - Li F
AD  - Department of Surgery, the First Affiliated Hospital of Guangxi Medical
      University, Nanning, Guangxi, China.
FAU - Li, Qian
AU  - Li Q
AD  - Department of Gynaecology and Obstetrics, the First Affiliated Hospital of
      Guangxi Medical University, Nanning, Guangxi, China.
FAU - Wu, Xianghua
AU  - Wu X
AUID- ORCID: 0000-0003-3467-3855
AD  - Department of Surgery, the First Affiliated Hospital of Guangxi Medical
      University, Nanning, Guangxi, China.
LA  - eng
PT  - Journal Article
DEP - 20180208
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Aged
MH  - Colonic Neoplasms/*genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - RNA, Long Noncoding/*genetics
PMC - PMC5805314
EDAT- 2018/02/09 06:00
MHDA- 2018/04/10 06:00
CRDT- 2018/02/09 06:00
PHST- 2017/08/18 00:00 [received]
PHST- 2018/01/24 00:00 [accepted]
PHST- 2018/02/09 06:00 [entrez]
PHST- 2018/02/09 06:00 [pubmed]
PHST- 2018/04/10 06:00 [medline]
AID - 10.1371/journal.pone.0192494 [doi]
AID - PONE-D-17-30575 [pii]
PST - epublish
SO  - PLoS One. 2018 Feb 8;13(2):e0192494. doi: 10.1371/journal.pone.0192494.
      eCollection 2018.