PMID- 29392319
OWN - NLM
STAT- MEDLINE
DCOM- 20180806
LR  - 20180806
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 59
IP  - 2
DP  - 2018 Feb 1
TI  - Nanosecond Laser Treatment for Age-Related Macular Degeneration Does Not Induce
      Focal Vision Loss or New Vessel Growth in the Retina.
PG  - 731-745
LID - 10.1167/iovs.17-23098 [doi]
AB  - Purpose: Subthreshold, nanosecond pulsed laser treatment shows promise as a
      treatment for age-related macular degeneration (AMD); however, the safety profile
      needs to be robustly examined. The aim of this study was to investigate the
      effects of laser treatment in humans and mice. Methods: Patients with AMD were
      treated with nanosecond pulsed laser at subthreshold (no visible retinal effect) 
      energy doses (0.15-0.45 mJ) and retinal sensitivity was assessed with
      microperimetry. Adult C57BL6J mice were treated at subthreshold (0.065 mJ) and
      suprathreshold (photoreceptor loss, 0.5 mJ) energy settings. The retinal and
      vascular responses were analyzed by fundus imaging, histologic assessment, and
      quantitative PCR. Results: Microperimetry analysis showed laser treatment had no 
      effect on retinal sensitivity under treated areas in patients 6 months to 7 years
      after treatment. In mice, subthreshold laser treatment induced RPE loss at 5
      hours, and by 7 days the RPE had retiled. Fundus imaging showed reduced RPE
      pigmentation but no change in retinal thickness up to 3 months. Electron
      microscopy revealed changes in melanosomes in the RPE, but Bruch's membrane was
      intact across the laser regions. Histologic analysis showed normal vasculature
      and no neovascularization. Suprathreshold laser treatment did not induce changes 
      in angiogenic genes associated with neovascularization. Instead pigment
      epithelium-derived factor, an antiangiogenic factor, was upregulated.
      Conclusions: In humans, low-energy, nanosecond pulsed laser treatment is not
      damaging to local retinal sensitivity. In mice, treatment does not damage Bruch's
      membrane or induce neovascularization, highlighting a reduced side effect profile
      of this nanosecond laser when used in a subthreshold manner.
FAU - Vessey, Kirstan A
AU  - Vessey KA
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
FAU - Ho, Tracy
AU  - Ho T
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
FAU - Jobling, Andrew I
AU  - Jobling AI
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
FAU - Mills, Samuel A
AU  - Mills SA
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
FAU - Tran, Mai X
AU  - Tran MX
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
FAU - Brandli, Alice
AU  - Brandli A
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
FAU - Lam, Jackson
AU  - Lam J
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
FAU - Guymer, Robyn H
AU  - Guymer RH
AD  - Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital;
      Department of Surgery (Ophthalmology), The University of Melbourne, Victoria,
      Australia.
FAU - Fletcher, Erica L
AU  - Fletcher EL
AD  - Department of Anatomy and Neuroscience, The University of Melbourne, Melbourne,
      Victoria, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Eye Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Serpins)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (pigment epithelium-derived factor)
RN  - 0 (vascular endothelial growth factor A, mouse)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Blindness/physiopathology/*prevention & control
MH  - Eye Proteins/genetics
MH  - Female
MH  - Fluorescein Angiography
MH  - Humans
MH  - Immunohistochemistry
MH  - Lasers, Solid-State/therapeutic use
MH  - *Low-Level Light Therapy
MH  - Macular Degeneration/physiopathology/*radiotherapy
MH  - Male
MH  - Melanosomes/ultrastructure
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Middle Aged
MH  - Nerve Growth Factors/genetics
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Retina/physiopathology
MH  - Retinal Neovascularization/physiopathology/*prevention & control
MH  - Retinal Pigment Epithelium/physiopathology
MH  - Serpins/genetics
MH  - Vascular Endothelial Growth Factor A/genetics
MH  - Visual Acuity/physiology
MH  - Visual Field Tests
EDAT- 2018/02/03 06:00
MHDA- 2018/08/07 06:00
CRDT- 2018/02/03 06:00
PHST- 2018/02/03 06:00 [entrez]
PHST- 2018/02/03 06:00 [pubmed]
PHST- 2018/08/07 06:00 [medline]
AID - 2671882 [pii]
AID - 10.1167/iovs.17-23098 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2018 Feb 1;59(2):731-745. doi: 10.1167/iovs.17-23098.