PMID- 29366593
OWN - NLM
STAT- In-Data-Review
LR  - 20180424
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Linking)
VI  - 319
DP  - 2018 Jun 15
TI  - Role of the chemokine receptors CXCR3, CXCR4 and CCR7 in the intramuscular
      recruitment of plasmacytoid dendritic cells in dermatomyositis.
PG  - 142-148
LID - S0165-5728(18)30015-8 [pii]
LID - 10.1016/j.jneuroim.2018.01.008 [doi]
AB  - To explore the possible mechanism implicated in the recruitment of plasmacytoid
      dendritic cells (pDCs), we investigated the expression of the chemokine receptors
      CXCR3, CXCR4, and CCR7 on intramuscular and circulating pDCs from patients with
      dermatomyositis (DM). Using immunohistochemistry, preferential expression of
      CXCR3, CXCR4 and CCR7 was identified in the perivascular inflammatory infiltrates
      within the perimysium in DM muscle. Western-blot analysis showed marked
      up-regulation of expression of CXCR3, CXCR4 and CCR7 in muscle homogenate from
      patients with DM compared with that in non-diseased controls. Co-localization of 
      CD303+ pDCs with these chemokine receptors was further examined by double
      immunofluorescence staining, which showed extensive co-localization of CD303 with
      CXCR3/CXCR4/CCR7 in DM biopsies. Flow cytometry was then used to investigate the 
      proportion of pDCs among the total PBMCs and the expression of CXCR3, CXCR4 and
      CCR7 on circulating pDCs. The proportion of CD123+CD303+ pDCs in peripheral blood
      from DM patients was markedly decreased compared to that from polymyositis (PM)
      patients and normal controls. Significantly increased expression of CXCR3, but
      not CXCR4 or CCR7, was further identified on circulating pDCs in DM. Correlation 
      analysis showed that the expression of CXCR3 correlated inversely with the
      frequency of pDCs in peripheral blood. Our findings indicate that the chemokine
      receptors, CXCR3, CXCR4 and CCR7 may be involved in the recruitment of pDCs from 
      peripheral blood to muscle tissues in DM via different mechanisms, and in which
      CXCR3 may play an important role under DM conditions.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Lv, Jingwei
AU  - Lv J
AD  - Department of Neurology and Neuromuscular Center, Qilu Hospital of Shandong
      University, Jinan, PR China.
FAU - Li, Ling
AU  - Li L
AD  - Department of Neurology and Neuromuscular Center, Qilu Hospital of Shandong
      University, Jinan, PR China.
FAU - Li, Wei
AU  - Li W
AD  - Department of Neurology and Neuromuscular Center, Qilu Hospital of Shandong
      University, Jinan, PR China.
FAU - Ji, Kunqian
AU  - Ji K
AD  - Department of Neurology and Neuromuscular Center, Qilu Hospital of Shandong
      University, Jinan, PR China.
FAU - Hou, Ying
AU  - Hou Y
AD  - Department of Neurology and Neuromuscular Center, Qilu Hospital of Shandong
      University, Jinan, PR China.
FAU - Yan, Chuanzhu
AU  - Yan C
AD  - Department of Neurology and Neuromuscular Center, Qilu Hospital of Shandong
      University, Jinan, PR China.
FAU - Dai, Tingjun
AU  - Dai T
AD  - Department of Neurology and Neuromuscular Center, Qilu Hospital of Shandong
      University, Jinan, PR China. Electronic address: tingjundai@126.com.
LA  - eng
PT  - Journal Article
DEP - 20180109
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
SB  - IM
OTO - NOTNLM
OT  - CCR7
OT  - CXCR3
OT  - CXCR4
OT  - Chemokine receptors
OT  - Dermatomyositis
OT  - Plasmacytoid dendritic cells
OT  - Type I interferon
EDAT- 2018/01/26 06:00
MHDA- 2018/01/26 06:00
CRDT- 2018/01/26 06:00
PHST- 2017/02/24 00:00 [received]
PHST- 2017/10/13 00:00 [revised]
PHST- 2018/01/08 00:00 [accepted]
PHST- 2018/01/26 06:00 [pubmed]
PHST- 2018/01/26 06:00 [medline]
PHST- 2018/01/26 06:00 [entrez]
AID - S0165-5728(18)30015-8 [pii]
AID - 10.1016/j.jneuroim.2018.01.008 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2018 Jun 15;319:142-148. doi: 10.1016/j.jneuroim.2018.01.008.
      Epub 2018 Jan 9.