PMID- 29343525
OWN - NLM
STAT- In-Data-Review
LR  - 20180330
IS  - 1524-4571 (Electronic)
IS  - 0009-7330 (Linking)
VI  - 122
IP  - 7
DP  - 2018 Mar 30
TI  - Lack of Remuscularization Following Transplantation of Human Embryonic Stem
      Cell-Derived Cardiovascular Progenitor Cells in Infarcted Nonhuman Primates.
PG  - 958-969
LID - 10.1161/CIRCRESAHA.117.311578 [doi]
AB  - RATIONALE: Human pluripotent stem cell-derived cardiovascular progenitor cells
      (hPSC-CVPCs) should be thoroughly investigated in large animal studies before
      testing in clinical trials. OBJECTIVE: The main of this study is to clarify
      whether hPSC-CVPCs can engraft for long time in the heart of primates after
      myocardial infarction (MI) and compare the effectiveness and safety of
      immunosuppression with cyclosporine alone or multiple-drug regimen (MDR)
      containing cyclosporine, methylprednisolone, and basiliximab in cynomolgus
      monkeys that had received intramyocardial injections of 1x10(7) EGFP (enhanced
      green fluorescent protein)-expressing hPSC-CVPCs after MI. A third group of
      animals received the immunosuppression MDR but without cell therapy after MI
      (MI+MDR group). METHODS AND RESULTS: Measurements of EGFP gene levels and EGFP
      immunofluorescence staining indicated that the hPSC-CVPC engraftment rate was
      greater in the MI+MDR+CVPC group than that in the MI+cyclosporine+CVPC group.
      However, even in the MI+MDR+CVPC group, no transplanted cells could be detected
      at 140 days after transplantation. Concomitantly, immunofluorescent analysis of
      CD3, CD4, and CD8 expression indicated that T-lymphocyte infiltration in the
      CVPC-transplanted hearts was less in the MDR-treated animals than in the
      cyclosporine-alone-treated animals. The recovery of left ventricular function on 
      day 28 post-MI in the MI+MDR+CVPC group was better than that in the MI+MDR group.
      Apoptotic cardiac cells were also less common in the MI+MDR+CVPC group than in
      the MI+MDR group, although both immunosuppression regimens were associated with
      transient hepatic dysfunction. CONCLUSIONS: This is the largest study of hPSCs in
      nonhuman primates in cardiovascular field to date (n=32). Compared with
      cyclosporine alone, MDR attenuates immune rejection and improves survival of
      hPSC-CVPCs in primates; this is associated with less apoptosis of native cardiac 
      cells and better recovery of left ventricular function at 28 days. However, even 
      with MDR, transplanted hPSC-CVPCs do not engraft and do not survive at 140 days
      after transplantation, thereby excluding remuscularization as a mechanism for the
      functional effect.
CI  - (c) 2018 American Heart Association, Inc.
FAU - Zhu, Keyang
AU  - Zhu K
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Wu, Qiang
AU  - Wu Q
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Ni, Cheng
AU  - Ni C
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Zhang, Peng
AU  - Zhang P
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Zhong, Zhiwei
AU  - Zhong Z
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Wu, Yan
AU  - Wu Y
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Wang, Yingchao
AU  - Wang Y
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Xu, Yinchuan
AU  - Xu Y
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Kong, Minjian
AU  - Kong M
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Cheng, Haifeng
AU  - Cheng H
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Tao, Zhihua
AU  - Tao Z
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Yang, Qian
AU  - Yang Q
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Liang, He
AU  - Liang H
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Jiang, Yun
AU  - Jiang Y
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Li, Qingju
AU  - Li Q
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Zhao, Jing
AU  - Zhao J
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Huang, Jijun
AU  - Huang J
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Zhang, Fengjiang
AU  - Zhang F
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Chen, Qi
AU  - Chen Q
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Li, Yi
AU  - Li Y
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Chen, Jinghai
AU  - Chen J
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Zhu, Wei
AU  - Zhu W
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Yu, Hong
AU  - Yu H
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Zhang, Jianyi
AU  - Zhang J
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang).
FAU - Yang, Huang-Tian
AU  - Yang HT
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang). wangjianan111@zju.edu.cn hxy0507@zju.edu.cn
      htyang@sibs.ac.cn.
FAU - Hu, Xinyang
AU  - Hu X
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang). wangjianan111@zju.edu.cn hxy0507@zju.edu.cn
      htyang@sibs.ac.cn.
FAU - Wang, Jian'an
AU  - Wang J
AD  - From the Department of Cardiology (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.Y., 
      Q.L., J. Zhao, J.C., W.Z., H.Y., X.H., J.W.), Department of Cardiovascular
      Surgery (M.K., H.C.), Department of Laboratory Medicine (Z.T.), and Department of
      Anesthesiology (F.Z., Q.C., Y.L.), Second Affiliated Hospital, College of
      Medicine, Zhejiang University, Hangzhou, PR China; Key Laboratory of Stem Cell
      Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences,
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CAS)
      Shanghai, University of CAS, Beijing, PR China (Q.W., P.Z., H.L., Y.J., J.H.,
      H.-T.Y.); Cardiovascular Key Laboratory of Zhejiang Province, Hangzhou, PR China 
      (K.Z., C.N., Z.Z., Y. Wu, Y. Wang, Y.X., Q.L., J. Zhao, J.C., W.Z., H.Y., X.H.,
      Y.W.); and Department of Biomedical Engineering, University of Alabama at
      Birmingham (J. Zhang). wangjianan111@zju.edu.cn hxy0507@zju.edu.cn
      htyang@sibs.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20180117
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
OTO - NOTNLM
OT  - human embryonic stem cells
OT  - immunosuppression
OT  - primates
OT  - transplantation
EDAT- 2018/01/19 06:00
MHDA- 2018/01/19 06:00
CRDT- 2018/01/19 06:00
PHST- 2017/06/23 00:00 [received]
PHST- 2018/01/09 00:00 [revised]
PHST- 2018/01/12 00:00 [accepted]
PHST- 2018/01/19 06:00 [pubmed]
PHST- 2018/01/19 06:00 [medline]
PHST- 2018/01/19 06:00 [entrez]
AID - CIRCRESAHA.117.311578 [pii]
AID - 10.1161/CIRCRESAHA.117.311578 [doi]
PST - ppublish
SO  - Circ Res. 2018 Mar 30;122(7):958-969. doi: 10.1161/CIRCRESAHA.117.311578. Epub
      2018 Jan 17.