PMID- 29305957
OWN - NLM
STAT- MEDLINE
DCOM- 20181011
LR  - 20181011
IS  - 1872-7913 (Electronic)
IS  - 0924-8579 (Linking)
VI  - 51
IP  - 6
DP  - 2018 Jun
TI  - Isolation of an IncP-1 plasmid harbouring mcr-1 from a chicken isolate of
      Citrobacter braakii in China.
PG  - 936-940
LID - S0924-8579(17)30473-9 [pii]
LID - 10.1016/j.ijantimicag.2017.12.030 [doi]
AB  - The plasmid-mediated colistin resistance gene mcr-1 has been found worldwide, but
      the diversity of organisms harbouring this gene is unknown. In this study, 12
      colistin-resistant Citrobacter spp. isolates were obtained from diseased or dead 
      chickens in China, and PCR analysis indicated that five were positive for mcr-1. 
      One Citrobacter braakii strain (SCC4) with a multidrug-resistant phenotype was
      chosen for further analysis. SCC4 was resistant or intermediate-resistant to ten 
      of the tested antibiotics, and the colistin minimum inhibitory concentration
      (MIC) was >4 microg/mL. A conjugation assay demonstrated successful transfer of
      colistin resistance to Escherichia coli strain J53 at a frequency of 10(-7) cells
      per recipient cell. Whole-genome sequencing revealed that SCC4 contained 13
      antibiotic resistance genes in its genome, and the mcr-1 gene resided on a 44-kb 
      self-transmissible IncP-type plasmid of a recently discovered IncP-1 clade. In
      addition, the mcr-1 gene was part of an insertion element
      (ISApl1-mcr-1-orf-ISApl1) that was excised from the plasmid as a circular
      intermediate form. This is the first report of mcr-1-posiitve C. braakii of
      animal origin and these findings highlight the fact that the mcr-1 gene can be
      found in normal enteric flora as part of broad-host-range plasmids.
CI  - Copyright (c) 2017 Elsevier B.V. and International Society of Chemotherapy. All
      rights reserved.
FAU - Liu, Jinxin
AU  - Liu J
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,
      College of Life Sciences, Sichuan University, Chengdu, China; Paul G. Allen
      School for Global Animal Health, College of Veterinary Medicine, Washington State
      University, Pullman, WA, USA.
FAU - Yang, Yanxian
AU  - Yang Y
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,
      College of Life Sciences, Sichuan University, Chengdu, China.
FAU - Li, Yunxia
AU  - Li Y
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,
      College of Life Sciences, Sichuan University, Chengdu, China.
FAU - Liu, Dan
AU  - Liu D
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,
      College of Life Sciences, Sichuan University, Chengdu, China.
FAU - Tuo, Hongmei
AU  - Tuo H
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,
      College of Life Sciences, Sichuan University, Chengdu, China.
FAU - Wang, Hongning
AU  - Wang H
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,
      College of Life Sciences, Sichuan University, Chengdu, China.
FAU - Call, Douglas R
AU  - Call DR
AD  - Paul G. Allen School for Global Animal Health, College of Veterinary Medicine,
      Washington State University, Pullman, WA, USA.
FAU - Davis, Margaret
AU  - Davis M
AD  - Paul G. Allen School for Global Animal Health, College of Veterinary Medicine,
      Washington State University, Pullman, WA, USA.
FAU - Zhang, Anyun
AU  - Zhang A
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,
      College of Life Sciences, Sichuan University, Chengdu, China; Paul G. Allen
      School for Global Animal Health, College of Veterinary Medicine, Washington State
      University, Pullman, WA, USA. Electronic address: zhanganyun@scu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180103
PL  - Netherlands
TA  - Int J Antimicrob Agents
JT  - International journal of antimicrobial agents
JID - 9111860
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA Transposable Elements)
RN  - EC 2.7.8.1 (Ethanolaminephosphotransferase)
RN  - Z67X93HJG1 (Colistin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Chickens
MH  - Citrobacter/*drug effects/*genetics/isolation & purification
MH  - Colistin/*pharmacology
MH  - DNA Transposable Elements/*genetics
MH  - Drug Resistance, Multiple, Bacterial/*genetics
MH  - Ethanolaminephosphotransferase/*genetics
MH  - Gene Transfer, Horizontal/genetics
MH  - Genome, Bacterial/genetics
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Plasmids/*genetics
MH  - Whole Genome Sequencing
OTO - NOTNLM
OT  - Citrobacter braakii
OT  - Colistin resistance
OT  - mcr-1
EDAT- 2018/01/07 06:00
MHDA- 2018/10/12 06:00
CRDT- 2018/01/07 06:00
PHST- 2017/05/18 00:00 [received]
PHST- 2017/10/12 00:00 [revised]
PHST- 2017/12/28 00:00 [accepted]
PHST- 2018/01/07 06:00 [pubmed]
PHST- 2018/10/12 06:00 [medline]
PHST- 2018/01/07 06:00 [entrez]
AID - S0924-8579(17)30473-9 [pii]
AID - 10.1016/j.ijantimicag.2017.12.030 [doi]
PST - ppublish
SO  - Int J Antimicrob Agents. 2018 Jun;51(6):936-940. doi:
      10.1016/j.ijantimicag.2017.12.030. Epub 2018 Jan 3.