PMID- 29128752
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20180522
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 514
DP  - 2018 Jan 15
TI  - The roles of five conserved lentiviral RNA structures in HIV-1 replication.
PG  - 1-8
LID - S0042-6822(17)30371-9 [pii]
LID - 10.1016/j.virol.2017.10.020 [doi]
AB  - The HIV-1 RNA genome contains complex structures with many structural elements
      playing regulatory roles during viral replication. A recent study has identified 
      multiple RNA structures with unknown functions that are conserved among HIV-1 and
      two simian immunodeficiency viruses. To explore the roles of these conserved RNA 
      structures, we introduced synonymous mutations into the HIV-1 genome to disrupt
      each structure. These mutants exhibited similar particle production, viral
      infectivity, and replication kinetics relative to the parent NL4-3 virus.
      However, when replicating in direct competition with the wild-type NL4-3 virus,
      mutations of RNA structures at inter-protein domain junctions can cause fitness
      defects. These findings reveal the ability of HIV-1 to tolerate changes in its
      sequences, even in apparently highly conserved structures, which permits high
      genetic diversity in HIV-1 population. Our results also suggest that some
      conserved RNA structures may function to fine-tune viral replication.
CI  - Published by Elsevier Inc.
FAU - Liu, Yang
AU  - Liu Y
AD  - Viral Recombination Section, HIV Dynamics and Replication Program, National
      Cancer Institute, Frederick, MD 21702, USA.
FAU - Chen, Jianbo
AU  - Chen J
AD  - Viral Recombination Section, HIV Dynamics and Replication Program, National
      Cancer Institute, Frederick, MD 21702, USA.
FAU - Nikolaitchik, Olga A
AU  - Nikolaitchik OA
AD  - Viral Recombination Section, HIV Dynamics and Replication Program, National
      Cancer Institute, Frederick, MD 21702, USA.
FAU - Desimmie, Belete A
AU  - Desimmie BA
AD  - Viral Mutation Section, HIV Dynamics and Replication Program, National Cancer
      Institute, Frederick, MD 21702, USA.
FAU - Busan, Steven
AU  - Busan S
AD  - Department of Chemistry, University of North Carolina, Chapel Hill, NC, USA.
FAU - Pathak, Vinay K
AU  - Pathak VK
AD  - Viral Mutation Section, HIV Dynamics and Replication Program, National Cancer
      Institute, Frederick, MD 21702, USA.
FAU - Weeks, Kevin M
AU  - Weeks KM
AD  - Department of Chemistry, University of North Carolina, Chapel Hill, NC, USA.
FAU - Hu, Wei-Shau
AU  - Hu WS
AD  - Viral Recombination Section, HIV Dynamics and Replication Program, National
      Cancer Institute, Frederick, MD 21702, USA. Electronic address:
      Wei-Shau.Hu@nih.gov.
LA  - eng
GR  - ZIA BC010814-10/NULL/Intramural NIH HHS/United States
GR  - ZIA BC010504-14/NULL/Intramural NIH HHS/United States
GR  - R01 AI068462/AI/NIAID NIH HHS/United States
GR  - ZIA BC010506-14/NULL/Intramural NIH HHS/United States
GR  - ZIA BC010533-08/NULL/Intramural NIH HHS/United States
GR  - ZIA BC011436-05/NULL/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20171109
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Conserved Sequence
MH  - HIV Infections/virology
MH  - HIV-1/chemistry/*genetics/physiology
MH  - Humans
MH  - Inverted Repeat Sequences
MH  - Lentivirus/chemistry/classification/*genetics/physiology
MH  - Lentivirus Infections/virology
MH  - Nucleic Acid Conformation
MH  - RNA, Viral/*chemistry/genetics/metabolism
MH  - Virus Replication
PMC - PMC5766380
MID - NIHMS919387
OTO - NOTNLM
OT  - *HIV
OT  - *Lentivirus
OT  - *RNA structure
OT  - *Replication fitness
OT  - *Viral replication
EDAT- 2017/11/13 06:00
MHDA- 2018/05/23 06:00
CRDT- 2017/11/13 06:00
PMCR- 2019/01/15 00:00
PHST- 2017/08/23 00:00 [received]
PHST- 2017/10/29 00:00 [revised]
PHST- 2017/10/31 00:00 [accepted]
PHST- 2019/01/15 00:00 [pmc-release]
PHST- 2017/11/13 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2017/11/13 06:00 [entrez]
AID - S0042-6822(17)30371-9 [pii]
AID - 10.1016/j.virol.2017.10.020 [doi]
PST - ppublish
SO  - Virology. 2018 Jan 15;514:1-8. doi: 10.1016/j.virol.2017.10.020. Epub 2017 Nov 9.