PMID- 29024811
OWN - NLM
STAT- MEDLINE
DCOM- 20171205
LR  - 20180522
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 412
DP  - 2018 Jan 1
TI  - CNOT2 promotes proliferation and angiogenesis via VEGF signaling in MDA-MB-231
      breast cancer cells.
PG  - 88-98
LID - S0304-3835(17)30621-3 [pii]
LID - 10.1016/j.canlet.2017.09.052 [doi]
AB  - Here the underlying role of CNOT2, a subunit of CCR4-NOT complex, was elucidated 
      in cancer progression. CNOT2 was overexpressed in HIT-T15, ASPC-1, BXPC-3, PC-3, 
      LNCaP, MCF-7 and MDA-MB-231 cell lines, which was confirmed by Tissue array in
      various human tumor tissues. Also, CNOT2 depletion suppressed proliferation and
      colony formation of MDA-MB-231 cells. Of note, microarray revealed decreased
      expression of CNOT2, VEGF-A, HIF2 alpha (<0.5 fold) and increased expression of
      UMOD1, LOC727847, MMP4, hCG and other genes (>2.0 fold) in CNOT2 depleted
      MDA-MB-231 cells compared to untreated control. Consistently, downregulation of
      VEGF, CNOT2 and HIF2 alpha was verified in CNOT2 depleted MDA-MB-231 cells by
      RT-qPCR. Additionally, CNOT2 depletion inhibited VEGF induced tube formation in
      HUVECs and reduced neovascularization in CAM assay. Furthermore, the growth of
      CNOT2 depleted MDA-MB-231 cells was significantly reduced in Balb/c nude mice
      along with decreased expression of VEGF and PCNA by immunohistochemistry compared
      to untreated control group. Overall, our findings provide evidences that CNOT2
      promotes proliferation and angiogenesis via VEGF signaling in MDA-MB-231 breast
      cancer cells as a potent molecular target for breast cancer treatment.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Sohn, Eun Jung
AU  - Sohn EJ
AD  - Cancer Molecular Targeted Herbal Research Center, College of Korean Medicine,
      Kyung Hee University, Seoul, South Korea.
FAU - Jung, Deok-Beom
AU  - Jung DB
AD  - Cancer Molecular Targeted Herbal Research Center, College of Korean Medicine,
      Kyung Hee University, Seoul, South Korea.
FAU - Lee, HyoJung
AU  - Lee H
AD  - Cancer Molecular Targeted Herbal Research Center, College of Korean Medicine,
      Kyung Hee University, Seoul, South Korea.
FAU - Han, In
AU  - Han I
AD  - Cancer Molecular Targeted Herbal Research Center, College of Korean Medicine,
      Kyung Hee University, Seoul, South Korea.
FAU - Lee, Jihyun
AU  - Lee J
AD  - Cancer Molecular Targeted Herbal Research Center, College of Korean Medicine,
      Kyung Hee University, Seoul, South Korea.
FAU - Lee, Hyemin
AU  - Lee H
AD  - Cancer Molecular Targeted Herbal Research Center, College of Korean Medicine,
      Kyung Hee University, Seoul, South Korea.
FAU - Kim, Sung-Hoon
AU  - Kim SH
AD  - Cancer Molecular Targeted Herbal Research Center, College of Korean Medicine,
      Kyung Hee University, Seoul, South Korea. Electronic address: sungkim7@khu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171009
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (CNOT2 protein, human)
RN  - 0 (Repressor Proteins)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
EIN - Cancer Lett. 2018 May 19;:. PMID: 29784502
MH  - Animals
MH  - Breast Neoplasms/blood supply/*pathology
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neovascularization, Pathologic/*etiology
MH  - Repressor Proteins/*physiology
MH  - Signal Transduction/*physiology
MH  - Vascular Endothelial Growth Factor A/*physiology
OTO - NOTNLM
OT  - *Breast cancer
OT  - *CAM
OT  - *CNOT2
OT  - *Tissue array
OT  - *VEGF
EDAT- 2017/10/13 06:00
MHDA- 2017/12/06 06:00
CRDT- 2017/10/13 06:00
PHST- 2017/09/02 00:00 [received]
PHST- 2017/09/27 00:00 [revised]
PHST- 2017/09/28 00:00 [accepted]
PHST- 2017/10/13 06:00 [pubmed]
PHST- 2017/12/06 06:00 [medline]
PHST- 2017/10/13 06:00 [entrez]
AID - S0304-3835(17)30621-3 [pii]
AID - 10.1016/j.canlet.2017.09.052 [doi]
PST - ppublish
SO  - Cancer Lett. 2018 Jan 1;412:88-98. doi: 10.1016/j.canlet.2017.09.052. Epub 2017
      Oct 9.