PMID- 28991703
OWN - NLM
STAT- MEDLINE
DCOM- 20180611
LR  - 20180611
IS  - 1878-5883 (Electronic)
IS  - 0022-510X (Linking)
VI  - 381
DP  - 2017 Oct 15
TI  - Klotho gene expression decreases in peripheral blood mononuclear cells (PBMCs) of
      patients with relapsing-remitting multiple sclerosis.
PG  - 305-307
LID - S0022-510X(17)34293-4 [pii]
LID - 10.1016/j.jns.2017.09.012 [doi]
AB  - BACKGROUND: we recently showed that a hypothesized anti-aging and
      anti-inflammatory protein, namely Klotho, may contribute to the etiology and/or
      pathogenesis of multiple sclerosis (MS). In addition, Klotho function and its
      gene expression are dependent on inflammatory pathways. Accordingly, the aim of
      this study was to investigate the Klotho gene expression within peripheral blood 
      mononuclear cells (PBMCs) of patients with MS. METHODS: Altogether, 30 patients
      with relapsing-remitting MS (RRMS) along with 30 age and sex-matched healthy
      individuals were enrolled in this study. Blood samples were obtained from all
      participants and then PBMCs were isolated. The quantitative Real-Time PCR was
      carried out for Klotho mRNA derived from PBMCs. RESULTS: The results showed that 
      klotho gene expression in the PBMCs of patients with RRMS is nearly 2.5-fold less
      than healthy individuals (P=0.0006). CONCLUSION: This is the first study
      demonstrating a possible role of Klotho in the PBMCs of MS patients.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Karami, Masoumeh
AU  - Karami M
AD  - Department of Biochemistry, School of Medicine, AJA University of Medical
      Sciences, Tehran, Iran.
FAU - Mehrabi, Farzad
AU  - Mehrabi F
AD  - Department of Neurology, School of Medicine, AJA University of Medical Sciences, 
      Tehran, Iran.
FAU - Allameh, Abdolamir
AU  - Allameh A
AD  - Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Pahlevan Kakhki, Majid
AU  - Pahlevan Kakhki M
AD  - Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares
      University, Tehran, Iran.
FAU - Amiri, Mehdi
AU  - Amiri M
AD  - Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
FAU - Emami Aleagha, Mohammad Sajad
AU  - Emami Aleagha MS
AD  - Department of Biochemistry, School of Medicine, AJA University of Medical
      Sciences, Tehran, Iran. Electronic address: Sajad.emami@modares.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Observational Study
DEP - 20170909
PL  - Netherlands
TA  - J Neurol Sci
JT  - Journal of the neurological sciences
JID - 0375403
RN  - 0 (RNA, Messenger)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - EC 3.2.1.31 (klotho protein)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Female
MH  - Gene Expression
MH  - Glucuronidase/*metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Multiple Sclerosis, Relapsing-Remitting/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Retrospective Studies
OTO - NOTNLM
OT  - Klotho
OT  - Multiple sclerosis
OT  - Peripheral blood mononuclear cells
OT  - Real-Time PCR
EDAT- 2017/10/11 06:00
MHDA- 2018/06/12 06:00
CRDT- 2017/10/10 06:00
PHST- 2017/06/29 00:00 [received]
PHST- 2017/08/21 00:00 [revised]
PHST- 2017/09/08 00:00 [accepted]
PHST- 2017/10/10 06:00 [entrez]
PHST- 2017/10/11 06:00 [pubmed]
PHST- 2018/06/12 06:00 [medline]
AID - S0022-510X(17)34293-4 [pii]
AID - 10.1016/j.jns.2017.09.012 [doi]
PST - ppublish
SO  - J Neurol Sci. 2017 Oct 15;381:305-307. doi: 10.1016/j.jns.2017.09.012. Epub 2017 
      Sep 9.