PMID- 28964306
OWN - NLM
STAT- MEDLINE
DCOM- 20171023
LR  - 20181202
IS  - 1872-8464 (Electronic)
IS  - 0165-5876 (Linking)
VI  - 101
DP  - 2017 Oct
TI  - Expression of calcium-binding proteins S100A8, S100A9 and S100A12 in otitis
      media.
PG  - 30-36
LID - S0165-5876(17)30338-5 [pii]
LID - 10.1016/j.ijporl.2017.07.025 [doi]
AB  - OBJECTIVES: Calgranulins (calcium-binding proteins S100A8, S100A9 and S100A12)
      are predominant cytoplasmic proteins of neutrophils and produced by various
      cells, playing multiple functions in innate immunity and the inflammatory
      process. Although up-regulated expression of S100A8 and S100A9 genes were
      observed in an animal model of otitis media (OM), their expressions have not been
      studied in human middle ear epithelial cells in response to the OM pathogen or in
      patients with recurrent or chronic OM (recurrent OM/RecOM or chronic OM with
      effusion/COME). STUDY DESIGN: Gene expressions were compared between
      Streptococcus pneumoniae (SP)-infected and non-infected human middle ear
      epithelial cells (HMEECs) as well as between chronic OM patients and control
      patients (CI). METHODS: Gene expressions were profiled by quantitative real time 
      PCR (qPCR). S100 proteins in OM patient and CI middle ear biopsies were detected 
      by immunostaining. RESULTS: S100A8, S100A9 and S100A12 gene expressions were
      elevated in SP-infected HMEECs in time-dependent manner. S100A8 and S100A9 but
      not S100A12 gene expression was significantly elevated in the middle ear mucosa
      of OM patients. S100A8 and S100A9 protein were observed in middle ear mucosa of
      OM, but not CI patients. Minimal co-localization was observed between S100A8 and 
      S100A9 with neutrophil elastase and cytokeratin in ME sections of OM patients.
      CONCLUSION: Elevated S100A8 and S100A9 gene expression in SP-infected HMEECs and 
      in the middle ear mucosa of OM, minor co-localized with neutrophil markers
      suggests that middle ear epithelial cell secretion of S100A8 and S100A9 may play 
      a role in the pathogenesis of recurrent and chronic OM.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Hong, Wenzhou
AU  - Hong W
AD  - Department of Otolaryngology and Communication Sciences, Medical College of
      Wisconsin, Milwaukee, WI 53226, USA. Electronic address: whong@mcw.edu.
FAU - Khampang, Pawjai
AU  - Khampang P
AD  - Department of Otolaryngology and Communication Sciences, Medical College of
      Wisconsin, Milwaukee, WI 53226, USA.
FAU - Samuels, Tina L
AU  - Samuels TL
AD  - Department of Otolaryngology and Communication Sciences, Medical College of
      Wisconsin, Milwaukee, WI 53226, USA.
FAU - Kerschner, Joseph E
AU  - Kerschner JE
AD  - Department of Otolaryngology and Communication Sciences, Medical College of
      Wisconsin, Milwaukee, WI 53226, USA; Division of Pediatric Otolaryngology,
      Medical College of Wisconsin, Children's Hospital of Wisconsin, Milwaukee, WI,
      USA.
FAU - Yan, Ke
AU  - Yan K
AD  - Division of Quantitative Health Sciences, Department of Pediatrics, Medical
      College of Wisconsin, Milwaukee, WI 53226, USA.
FAU - Simpson, Pippa
AU  - Simpson P
AD  - Division of Quantitative Health Sciences, Department of Pediatrics, Medical
      College of Wisconsin, Milwaukee, WI 53226, USA.
LA  - eng
GR  - R01 DC007903/DC/NIDCD NIH HHS/United States
PT  - Journal Article
DEP - 20170721
PL  - Ireland
TA  - Int J Pediatr Otorhinolaryngol
JT  - International journal of pediatric otorhinolaryngology
JID - 8003603
RN  - 0 (Biomarkers)
RN  - 0 (S100 Proteins)
SB  - IM
MH  - Biomarkers
MH  - Chronic Disease
MH  - Ear, Middle/*metabolism/microbiology
MH  - Epithelial Cells/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Otitis Media/*metabolism/microbiology
MH  - Pneumococcal Infections/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - S100 Proteins/*metabolism
MH  - Streptococcus pneumoniae
OTO - NOTNLM
OT  - Calcium-binding proteins
OT  - Gene expression
OT  - Otitis media
OT  - Patients
EDAT- 2017/10/02 06:00
MHDA- 2017/10/24 06:00
CRDT- 2017/10/02 06:00
PHST- 2017/06/01 00:00 [received]
PHST- 2017/07/18 00:00 [revised]
PHST- 2017/07/19 00:00 [accepted]
PHST- 2017/10/02 06:00 [entrez]
PHST- 2017/10/02 06:00 [pubmed]
PHST- 2017/10/24 06:00 [medline]
AID - S0165-5876(17)30338-5 [pii]
AID - 10.1016/j.ijporl.2017.07.025 [doi]
PST - ppublish
SO  - Int J Pediatr Otorhinolaryngol. 2017 Oct;101:30-36. doi:
      10.1016/j.ijporl.2017.07.025. Epub 2017 Jul 21.