PMID- 28903577
OWN - NLM
STAT- MEDLINE
DCOM- 20180130
LR  - 20180308
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Linking)
VI  - 33
IP  - 12
DP  - 2017 Dec
TI  - A 2-4-Amino Acid Deletion in the V5 Region of HIV-1 Env gp120 Confers Viral
      Resistance to the Broadly Neutralizing Human Monoclonal Antibody, VRC01.
PG  - 1248-1257
LID - 10.1089/aid.2017.0063 [doi]
AB  - The envelope glycoprotein (Env) gp120 of human immunodeficiency virus type 1
      (HIV-1) plays a critical role in viral entry into host cells. The broadly
      neutralizing human monoclonal antibody VRC01, which recognizes the CD4 binding
      site on gp120, neutralizes more than 90% of HIV-1 isolates. However, some of the 
      CRF01_AE viruses prevalent in Southeast Asia are resistant to VRC01-mediated
      neutralization. We previously reported that 3 amino acid residues at positions
      185, 186, and 197 of gp120 played an important role in the VRC01 resistance of
      CRF01_AE Env (AE-Env) clones isolated from HIV-infected Thai individuals.
      However, the VRC01 susceptibility of AE-Env clones was not fully explained by
      mutations at these 3 residues. In the present study, we examined other factors
      involved in the acquisition of viral VRC01 resistance. Neutralization tests using
      lentiviral vectors expressing a series of mutant AE-Env clones revealed that the 
      deletion of 2-4 amino acid residues on the loop structure in the V5 region of
      gp120 conferred VRC01 resistance to several AE-Env clones. Our results provide
      novel insights into the mechanisms underlying viral VRC01 resistance.
FAU - Tachibana, Shingo
AU  - Tachibana S
AD  - 1 Department of International Health, Kobe University Graduate School of Health
      Sciences, Kobe University , Kobe, Japan .
FAU - Sasaki, Maho
AU  - Sasaki M
AD  - 1 Department of International Health, Kobe University Graduate School of Health
      Sciences, Kobe University , Kobe, Japan .
FAU - Tanaka, Takako
AU  - Tanaka T
AD  - 2 Medical Technology Major, Kobe University School of Medicine Faculty of Health 
      Sciences, School of Medicine, Kobe University , Kobe, Japan .
FAU - Inoue, Mari
AU  - Inoue M
AD  - 1 Department of International Health, Kobe University Graduate School of Health
      Sciences, Kobe University , Kobe, Japan .
FAU - Ophinni, Youdiil
AU  - Ophinni Y
AD  - 3 Division of Vaccinology, Center for Infectious Diseases, Kobe University
      Graduate School of Medicine, Kobe University , Kobe, Japan .
FAU - Kotaki, Tomohiro
AU  - Kotaki T
AD  - 1 Department of International Health, Kobe University Graduate School of Health
      Sciences, Kobe University , Kobe, Japan .
FAU - Kameoka, Masanori
AU  - Kameoka M
AD  - 1 Department of International Health, Kobe University Graduate School of Health
      Sciences, Kobe University , Kobe, Japan .
AD  - 3 Division of Vaccinology, Center for Infectious Diseases, Kobe University
      Graduate School of Medicine, Kobe University , Kobe, Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171017
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Anti-HIV Agents)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (HIV Antibodies)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (VRC01 monoclonal antibody)
SB  - IM
SB  - X
MH  - Amino Acid Sequence
MH  - Anti-HIV Agents/immunology/*therapeutic use
MH  - Antibodies, Monoclonal/immunology/*therapeutic use
MH  - Antibodies, Neutralizing/immunology/*therapeutic use
MH  - Binding Sites/genetics
MH  - Cell Line
MH  - Drug Resistance, Viral/*genetics
MH  - HIV Antibodies/immunology/*therapeutic use
MH  - HIV Envelope Protein gp120/*genetics
MH  - HIV Infections/*drug therapy/virology
MH  - HIV-1/genetics/immunology
MH  - Humans
MH  - Neutralization Tests
MH  - Sequence Deletion/genetics
MH  - Thailand
MH  - Virus Internalization
OTO - NOTNLM
OT  - *CRF01_AE
OT  - *HIV-1
OT  - *V5 region
OT  - *VRC01
OT  - *broadly neutralizing antibody
OT  - *gp120
EDAT- 2017/09/15 06:00
MHDA- 2018/01/31 06:00
CRDT- 2017/09/15 06:00
PHST- 2017/09/15 06:00 [pubmed]
PHST- 2018/01/31 06:00 [medline]
PHST- 2017/09/15 06:00 [entrez]
AID - 10.1089/aid.2017.0063 [doi]
AID - 10.1089/AID.2017.0063 [pii]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2017 Dec;33(12):1248-1257. doi: 10.1089/aid.2017.0063.
      Epub 2017 Oct 17.