PMID- 28807821
OWN - NLM
STAT- MEDLINE
DCOM- 20170921
LR  - 20180415
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 406
DP  - 2017 Oct 10
TI  - POPDC1 is suppressed in human breast cancer tissues and is negatively regulated
      by EGFR in breast cancer cell lines.
PG  - 81-92
LID - S0304-3835(17)30479-2 [pii]
LID - 10.1016/j.canlet.2017.08.002 [doi]
AB  - Breast cancer molecular heterogeneity has resulted in disparities in therapeutic 
      response and targeting of molecular subtypes of breast cancer. This necessitates 
      identification and validation of novel therapeutic targets for breast cancer
      treatment. Suppression of Popeye domain-containing (POPDC) proteins is
      hypothesized to promote malignant cell behaviour and poor clinical outcomes in
      various cancers. We aimed to determine whether POPDC proteins are suppressed in
      human ductal carcinoma tissues and if this correlates to clinical progression and
      Her2 status. We further assessed if the EGFR regulated POPDC1 in breast cancer.
      Here we show significant suppression of POPDC1 in malignant breast cancer tissues
      without correlation to clinical progression. Interestingly, POPDC2 and POPDC3
      were highly expressed in malignant breast tissues. Furthermore, HER2+ status
      significantly correlated with high POPDC2 and POPDC3, but not POPDC1 expression. 
      We further show for the first time that low POPDC1 correlates to high EGFR
      expression in breast cancer tissues and that EGFR negatively regulates POPDC1
      expression in MCF7, MDA231 and SKBR3 breast cancer cells. Furthermore,
      overexpression of POPDC1 in MCF7, MDA231 and SKBR3 cells attenuated EGF-mediated 
      cell migration and proliferation. These findings show that POPDC1 is suppressed
      in breast cancer and can potentially be targeted to inhibit EGFR-mediated cell
      migration and proliferation.
CI  - Crown Copyright (c) 2017. Published by Elsevier B.V. All rights reserved.
FAU - Amunjela, Johanna Ndamwena
AU  - Amunjela JN
AD  - School of Medicine, Medical Sciences and Nutrition, University of Aberdeen,
      Foresterhill, Aberdeen AB25 2ZD, Scotland, United Kingdom. Electronic address:
      j.n.amunjela@abdn.ac.uk.
FAU - Tucker, Steven John
AU  - Tucker SJ
AD  - School of Medicine, Medical Sciences and Nutrition, University of Aberdeen,
      Foresterhill, Aberdeen AB25 2ZD, Scotland, United Kingdom. Electronic address:
      s.j.tucker@abdn.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170812
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (BVES protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Membrane Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (POPDC2 protein, human)
RN  - 0 (POPDC3 protein, human)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers, Tumor/*metabolism
MH  - Blotting, Western
MH  - Breast Neoplasms/metabolism/*pathology
MH  - Carcinoma, Ductal, Breast/metabolism/*pathology
MH  - Carcinoma, Lobular/metabolism/*pathology
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Humans
MH  - Membrane Proteins/*metabolism
MH  - Muscle Proteins/metabolism
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Receptor, Epidermal Growth Factor/*metabolism
MH  - Receptor, ErbB-2/metabolism
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - *EGFR
OT  - *HER2
OT  - *POPDC1
OT  - *POPDC2
OT  - *POPDC3
EDAT- 2017/08/16 06:00
MHDA- 2017/09/22 06:00
CRDT- 2017/08/16 06:00
PHST- 2017/05/13 00:00 [received]
PHST- 2017/07/31 00:00 [revised]
PHST- 2017/08/04 00:00 [accepted]
PHST- 2017/08/16 06:00 [pubmed]
PHST- 2017/09/22 06:00 [medline]
PHST- 2017/08/16 06:00 [entrez]
AID - S0304-3835(17)30479-2 [pii]
AID - 10.1016/j.canlet.2017.08.002 [doi]
PST - ppublish
SO  - Cancer Lett. 2017 Oct 10;406:81-92. doi: 10.1016/j.canlet.2017.08.002. Epub 2017 
      Aug 12.