PMID- 28797112
OWN - NLM
STAT- MEDLINE
DCOM- 20171004
LR  - 20181202
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - A novel whole-bacterial enzyme linked-immunosorbant assay to quantify Chlamydia
      trachomatis specific antibodies reveals distinct differences between systemic and
      genital compartments.
PG  - e0183101
LID - 10.1371/journal.pone.0183101 [doi]
AB  - Chlamydia trachomatis (CT) is the leading sexually transmitted bacterial
      infection. The continued global burden of CT infection strongly predicates the
      need for a vaccine to supplement current chlamydial control programs. The
      correlates of protection against CT are currently unknown, but they must be
      carefully defined to guide vaccine design. The localized nature of chlamydial
      infection in columnar epithelial cells of the genital tract necessitates
      investigation of immunity at the site of infection. The purpose of this study was
      to develop a sensitive whole bacterial enzyme-linked immunosorbent assay (ELISA) 
      to quantify and compare CT-specific IgG and IgA in sera and genital secretions
      from CT-infected women. To achieve this, elementary bodies (EBs) from two of the 
      most common genital serovars (D and E) were attached to poly-L-lysine-coated
      microtiter plates with glutaraldehyde. EB attachment and integrity were verified 
      by the presence of outer membrane antigens and the absence of bacterial
      cytoplasmic antigens. EB-specific IgG and IgA standards were developed by pooling
      sera with high titers of CT-specific antibodies from infected women. Serum,
      endocervical and vaginal secretions, and endocervical cytobrush specimens from
      CT-infected women were used to quantify CT-specific IgG and IgA which were then
      normalized to total IgG and IgA, respectively. Analyses of paired serum and
      genital samples revealed significantly higher proportions of EB-specific
      antibodies in genital secretions compared to sera. Cervical and vaginal
      secretions and cytobrush specimens had similar proportions of EB-specific
      antibodies, suggesting any one of these genital sampling techniques could be used
      to quantify CT-specific antibodies when appropriate normalization methodologies
      are implemented. Overall, these results illustrate the need to investigate
      genital tract CT antibody responses, and our assay provides a useful quantitative
      tool to assess natural immunity in defined clinical groups and CT vaccine trials.
FAU - Albritton, Hannah L
AU  - Albritton HL
AD  - Department of Microbiology, Immunology, and Parasitology, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - Kozlowski, Pamela A
AU  - Kozlowski PA
AD  - Department of Microbiology, Immunology, and Parasitology, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - Lillis, Rebecca A
AU  - Lillis RA
AD  - Department of Medicine, Division of Infectious Diseases, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - McGowin, Chris L
AU  - McGowin CL
AD  - Department of Microbiology, Immunology, and Parasitology, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - Siren, Julia D
AU  - Siren JD
AD  - Department of Medicine, Division of Infectious Diseases, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - Taylor, Stephanie N
AU  - Taylor SN
AD  - Department of Medicine, Division of Infectious Diseases, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - Ibana, Joyce A
AU  - Ibana JA
AD  - Department of Microbiology, Immunology, and Parasitology, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
AD  - Institute of Biology, University of the Philippines Diliman, Quezon City,
      National Capital Region, Philippines.
FAU - Buckner, Lyndsey R
AU  - Buckner LR
AD  - Department of Microbiology, Immunology, and Parasitology, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - Shen, Li
AU  - Shen L
AD  - Department of Microbiology, Immunology, and Parasitology, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
FAU - Quayle, Alison J
AU  - Quayle AJ
AUID- ORCID: http://orcid.org/0000-0003-2313-1292
AD  - Department of Microbiology, Immunology, and Parasitology, Louisiana State
      University Health Sciences Center, New Orleans, LA, United States of America.
LA  - eng
GR  - U54 GM104940/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20170810
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antibodies, Bacterial/analysis/blood/*immunology
MH  - Cell Line
MH  - Cervix Uteri/immunology/metabolism/microbiology
MH  - Chlamydia Infections/blood/*immunology
MH  - Chlamydia trachomatis/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin A/analysis/blood/immunology
MH  - Immunoglobulin G/analysis/blood/immunology
MH  - Mice
MH  - Vagina/immunology/metabolism/microbiology
MH  - Young Adult
PMC - PMC5552291
EDAT- 2017/08/11 06:00
MHDA- 2017/10/05 06:00
CRDT- 2017/08/11 06:00
PHST- 2017/05/24 00:00 [received]
PHST- 2017/07/29 00:00 [accepted]
PHST- 2017/08/11 06:00 [entrez]
PHST- 2017/08/11 06:00 [pubmed]
PHST- 2017/10/05 06:00 [medline]
AID - 10.1371/journal.pone.0183101 [doi]
AID - PONE-D-17-19798 [pii]
PST - epublish
SO  - PLoS One. 2017 Aug 10;12(8):e0183101. doi: 10.1371/journal.pone.0183101.
      eCollection 2017.