PMID- 28763467
OWN - NLM
STAT- MEDLINE
DCOM- 20171017
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - Effects of TNF-alpha on penile structure alteration in rats with
      hyperprolactinemia.
PG  - e0181952
LID - 10.1371/journal.pone.0181952 [doi]
AB  - This study aimed to investigate the effects of anti-tumor necrosis factor
      (TNF)-alpha antibody (Ab) on alteration of penile structure in the
      hyperprolactinemia (hyperPRL) rat model. HyperPRL was induced in 8-week-old male 
      Sprague-Dawley rats by allografting anterior pituitary (AP) glands under the
      renal capsule (+AP rats). Rats implanted with cerebral cortex (CX) were used as
      sham control (+CX rats). At 6 weeks post implantation, rats received either a
      single intra-testicular dose of TNF-alpha Ab (12.5 mug/kg) or testosterone
      replacement (2 doses of testosterone enanthate [TE], 3 mg/kg), and they were
      sacrificed 1 week later. Blood and penile tissue was collected for analysis.
      Compared to +CX rats, the +AP group had lower serum testosterone concentration
      and neuronal nitric oxide synthase (nNOS) expression, but exhibited a higher
      ratio of collagen III/I in the corpus cavernosum. Smooth muscle content exhibited
      no significant changes. At 1 week post TNF-alpha Ab injection, the collagen III/I
      ratio in the +AP group was decreased, and the smooth muscle content and nNOS
      expression increased significantly. These findings were comparable to those
      observed in +AP rats receiving TE. Testicular TNF-alpha suppresses testosterone
      release, which in turn results in the erectile dysfunction (ED) seen in hyperPRL.
      Intra-testicular TNF-alpha Ab treatment is as effective as testosterone
      supplementation on penile structure normalization in the hyperPRL model.
FAU - Wang, Zhong-Lin
AU  - Wang ZL
AD  - Department of Physiology, School of Medicine, National Yang-Ming University,
      Taipei, Taiwan.
FAU - Yang, Ling-Yu
AU  - Yang LY
AD  - Department of Pediatrics, School of Medicine, National Yang-Ming University,
      Taipei, Taiwan.
AD  - Department of Medical Education, and Pediatrics, Taipei Veterans General
      Hospital, Taipei, Taiwan.
FAU - Chen, Hong-Huan
AU  - Chen HH
AD  - Department of Physiology, School of Medicine, National Yang-Ming University,
      Taipei, Taiwan.
FAU - Lin, Hsiao-Hsin
AU  - Lin HH
AD  - Department of Physiology, School of Medicine, National Yang-Ming University,
      Taipei, Taiwan.
FAU - Tsai, Yi-Ting
AU  - Tsai YT
AD  - Department of Physiology, School of Medicine, National Yang-Ming University,
      Taipei, Taiwan.
FAU - Huang, William J
AU  - Huang WJ
AUID- ORCID: http://orcid.org/0000-0001-9048-4990
AD  - Department of Physiology, School of Medicine, National Yang-Ming University,
      Taipei, Taiwan.
AD  - Department of Urology, School of Medicine, Shu-Tien Urological Research Center,
      National Yang-Ming University, Taipei, Taiwan.
AD  - Division of Male Reproductive Medicine, Department of Urology, Taipei Veterans
      General Hospital, Taipei, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20170801
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3XMK78S47O (Testosterone)
RN  - 9002-62-4 (Prolactin)
RN  - 9007-34-5 (Collagen)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, rat)
SB  - IM
MH  - Animals
MH  - Antibodies/*pharmacology
MH  - Cerebral Cortex/metabolism
MH  - Collagen/chemistry
MH  - Erectile Dysfunction/physiopathology
MH  - Hyperprolactinemia/*blood
MH  - Macrophages/metabolism
MH  - Male
MH  - Muscle, Smooth/metabolism
MH  - Nitric Oxide Synthase Type I/metabolism
MH  - Penile Erection
MH  - Penis/*pathology
MH  - Prolactin/blood/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testosterone/blood/metabolism
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors
PMC - PMC5538640
EDAT- 2017/08/02 06:00
MHDA- 2017/10/19 06:00
CRDT- 2017/08/02 06:00
PHST- 2016/10/14 00:00 [received]
PHST- 2017/07/10 00:00 [accepted]
PHST- 2017/08/02 06:00 [entrez]
PHST- 2017/08/02 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
AID - 10.1371/journal.pone.0181952 [doi]
AID - PONE-D-16-40993 [pii]
PST - epublish
SO  - PLoS One. 2017 Aug 1;12(8):e0181952. doi: 10.1371/journal.pone.0181952.
      eCollection 2017.