PMID- 28750069
OWN - NLM
STAT- MEDLINE
DCOM- 20171009
LR  - 20171009
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Does repeated pleural culture increase the diagnostic yield of Mycobacterium
      tuberculosis from tuberculous pleural effusion in HIV-negative individuals?
PG  - e0181798
LID - 10.1371/journal.pone.0181798 [doi]
AB  - BACKGROUND: Despite recent advances in methods for culturing Mycobacterium
      tuberculosis (MTB), the diagnostic yield of tuberculous pleural effusion (TBPE)
      remains unsatisfactory. However, unlike repeated sputum cultures of pulmonary
      tuberculosis, little is known about the role of repeated pleural cultures. We
      examined whether repeated pleural cultures are associated with increased MTB
      yield from TBPE. METHODS: A multicenter, retrospective cohort study was performed
      from January 2012 to December 2015 in South Korea. Patients were categorized into
      two groups: single- or repeated-culture groups. The diagnostic yield of MTB and
      clinical, radiological, and pleural fluid characteristics were evaluated.
      RESULTS: Among the 329 patients with TBPE, 77 (23.4%) had repeated cultures and
      252 (76.5%) had a single culture. Pleural culture was performed twice in all 77
      patients in the repeated-culture group at a 1-day interval (inter-quartile range,
      1.0-2.0). In the repeated-culture group, the yield of MTB from the first culture 
      was 31.2%, which was similar to that in the single-culture group (31.2% vs.
      29.8%, P = 0.887). However, the yield of MTB from the second culture (10/77,
      13.0%) was more than that from the first. These results may be attributable to
      the insufficient immune clearance for MTB invasion into the pleural space between
      the first and second cultures. Over time, the yield of the second cultures
      decreased from 17.4% to 6.7% and then 6.3%. Finally, the overall yield of MTB in 
      the repeated- and single-culture groups was 44.2% and 29.8% respectively (P <
      0.001). CONCLUSIONS: The results showed that repeated pleural cultures increased 
      MTB yield from TBPE in human immunodeficiency virus-negative individuals.
      Furthermore, repeated cultures may increase yield when carried out for two
      consecutive days.
FAU - Ko, Yousang
AU  - Ko Y
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal
      Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 
      Seoul, Republic of Korea.
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
FAU - Song, Jinkyung
AU  - Song J
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal
      Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 
      Seoul, Republic of Korea.
FAU - Lee, Suh-Young
AU  - Lee SY
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal
      Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 
      Seoul, Republic of Korea.
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
FAU - Moon, Jin-Wook
AU  - Moon JW
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal
      Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 
      Seoul, Republic of Korea.
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
FAU - Mo, Eun-Kyung
AU  - Mo EK
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal
      Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 
      Seoul, Republic of Korea.
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
FAU - Park, Ji Young
AU  - Park JY
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Hallym University
      Sacred Heart Hospital, Anyang, Republic of Korea.
FAU - Kim, Joo-Hee
AU  - Kim JH
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Hallym University
      Sacred Heart Hospital, Anyang, Republic of Korea.
FAU - Park, Sunghoon
AU  - Park S
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Hallym University
      Sacred Heart Hospital, Anyang, Republic of Korea.
FAU - Hwang, Yong Il
AU  - Hwang YI
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Hallym University
      Sacred Heart Hospital, Anyang, Republic of Korea.
FAU - Jang, Seung Hun
AU  - Jang SH
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Hallym University
      Sacred Heart Hospital, Anyang, Republic of Korea.
FAU - Jhun, Byung Woo
AU  - Jhun BW
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Kangnam Sacred Heart
      Hospital, Seoul, Republic of Korea.
FAU - Sim, Yun Su
AU  - Sim YS
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Kangnam Sacred Heart
      Hospital, Seoul, Republic of Korea.
FAU - Shin, Tae Rim
AU  - Shin TR
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Kangnam Sacred Heart
      Hospital, Seoul, Republic of Korea.
FAU - Kim, Dong-Gyu
AU  - Kim DG
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Kangnam Sacred Heart
      Hospital, Seoul, Republic of Korea.
FAU - Hong, Ji Young
AU  - Hong JY
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Chuncheon Sacred Heart
      Hospital, Chuncheon, Republic of Korea.
FAU - Lee, Chang Youl
AU  - Lee CY
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Chuncheon Sacred Heart
      Hospital, Chuncheon, Republic of Korea.
FAU - Lee, Myung Goo
AU  - Lee MG
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Chuncheon Sacred Heart
      Hospital, Chuncheon, Republic of Korea.
FAU - Kim, Cheol-Hong
AU  - Kim CH
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Dongtan Sacred Heart
      Hospital, Hwaseong, Republic of Korea.
FAU - Hyun, In Gyu
AU  - Hyun IG
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Dongtan Sacred Heart
      Hospital, Hwaseong, Republic of Korea.
FAU - Park, Yong Bum
AU  - Park YB
AUID- ORCID: http://orcid.org/0000-0002-5814-6104
AD  - Division of Pulmonary, Allergy and Critical Care Medicine, Department of Internal
      Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, 
      Seoul, Republic of Korea.
AD  - Lung Research Institute of Hallym University College of Medicine, Chuncheon,
      Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20170727
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - HIV Infections/*complications/*microbiology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/*isolation & purification
MH  - Pleural Effusion/*complications/*microbiology
MH  - Tuberculosis, Pleural/*diagnosis
PMC - PMC5531521
EDAT- 2017/07/28 06:00
MHDA- 2017/10/11 06:00
CRDT- 2017/07/28 06:00
PHST- 2017/02/02 00:00 [received]
PHST- 2017/07/09 00:00 [accepted]
PHST- 2017/07/28 06:00 [entrez]
PHST- 2017/07/28 06:00 [pubmed]
PHST- 2017/10/11 06:00 [medline]
AID - 10.1371/journal.pone.0181798 [doi]
AID - PONE-D-17-04382 [pii]
PST - epublish
SO  - PLoS One. 2017 Jul 27;12(7):e0181798. doi: 10.1371/journal.pone.0181798.
      eCollection 2017.