PMID- 28750040
OWN - NLM
STAT- MEDLINE
DCOM- 20170929
LR  - 20170929
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Oral administration of live- or heat-killed Candida albicans worsened cecal
      ligation and puncture sepsis in a murine model possibly due to an increased serum
      (1-->3)-beta-D-glucan.
PG  - e0181439
LID - 10.1371/journal.pone.0181439 [doi]
AB  - Candida albicans is the most common fungus in the human intestinal microbiota but
      not in mice. To make a murine sepsis model more closely resemble human sepsis and
      to explore the role of intestinal C. albicans, in the absence of candidemia, in
      bacterial sepsis, live- or heat-killed C. albicans was orally administered to
      mice at 3h prior to cecal ligation and puncture (CLP). A higher mortality rate of
      CLP was demonstrated with Candida-administration (live- or heat-killed) prior to 
      CLP. Fecal Candida presented only in experiments with live-Candida
      administration. Despite the absence of candidemia, serum (1-->3)-beta-D-glucan
      (BG) was higher in CLP with Candida-administration than CLP-controls (normal
      saline administration) at 6h and/or 18h post-CLP. Interestingly, fluconazole
      attenuated the fecal Candida burden and improved survival in mice with
      live-Candida administration, but not CLP-control. Microbiota analysis revealed
      increased Bacteroides spp. and reduced Lactobacillus spp. in feces after Candida 
      administration. Additionally, synergy in the elicitation of cytokine production
      from bone marrow-derived macrophages, in vitro, was demonstrated by co-exposure
      to heat-killed E. coli and BG. In conclusion, intestinal abundance of fungi
      and/or fungal-molecules was associated with increased bacterial sepsis-severity, 
      perhaps through enhanced cytokine elicitation induced by synergistic responses to
      molecules from gut-derived bacteria and fungi. Conversely, reducing intestinal
      fungal burdens decreased serum BG and attenuated sepsis in our model.
FAU - Panpetch, Wimonrat
AU  - Panpetch W
AD  - Interdisciplinary Program of Medical Microbiology, Graduate school, Chulalongkorn
      University, Bangkok, Thailand.
AD  - Department of Microbiology, Faculty of Medicine, Chulalongkorn University,
      Bangkok, Thailand.
FAU - Somboonna, Naraporn
AU  - Somboonna N
AD  - Department of Microbiology, Faculty of Science, Chulalongkorn University,
      Bangkok, Thailand.
AD  - Omics Sciences and Bioinformatics Center, Faculty of Science, Chulalongkorn
      University, Bangkok, Thailand.
FAU - Bulan, Dewi Embong
AU  - Bulan DE
AD  - Department of Water Resources Management, Faculty of Fisheries and Marine
      Science, Mulawarman University, Indonesia.
FAU - Issara-Amphorn, Jiraphorn
AU  - Issara-Amphorn J
AD  - Department of Microbiology, Faculty of Medicine, Chulalongkorn University,
      Bangkok, Thailand.
FAU - Finkelman, Malcolm
AU  - Finkelman M
AD  - Associates of Cape Cod, Inc., East Falmouth, Massachusetts, United States of
      America.
FAU - Worasilchai, Navaporn
AU  - Worasilchai N
AD  - Department of Microbiology, Faculty of Medicine, Chulalongkorn University,
      Bangkok, Thailand.
FAU - Chindamporn, Ariya
AU  - Chindamporn A
AD  - Department of Microbiology, Faculty of Medicine, Chulalongkorn University,
      Bangkok, Thailand.
FAU - Palaga, Tanapat
AU  - Palaga T
AD  - Department of Microbiology, Faculty of Science, Chulalongkorn University,
      Bangkok, Thailand.
FAU - Tumwasorn, Somying
AU  - Tumwasorn S
AD  - Department of Microbiology, Faculty of Medicine, Chulalongkorn University,
      Bangkok, Thailand.
FAU - Leelahavanichkul, Asada
AU  - Leelahavanichkul A
AUID- ORCID: http://orcid.org/0000-0002-5566-6403
AD  - Department of Microbiology, Faculty of Medicine, Chulalongkorn University,
      Bangkok, Thailand.
AD  - Center of Excellence in Immunology and Immune-mediated Diseases, Department of
      Microbiology, Faculty of Medicine, Bangkok, Thailand.
AD  - STAR on Craniofacial and Skeleton Disorders, Faculty of Dentistry, Chulalongkorn 
      University, Bangkok, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20170727
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cytokines)
RN  - 0 (beta-Glucans)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Blood Bactericidal Activity
MH  - Candida albicans/*physiology
MH  - Cecum/*pathology
MH  - Cytokines/blood
MH  - Disease Models, Animal
MH  - Escherichia coli/metabolism
MH  - Feces/microbiology
MH  - Gastrointestinal Microbiome
MH  - Ligation
MH  - Macrophages/metabolism
MH  - Male
MH  - Mice, Inbred ICR
MH  - Punctures
MH  - Sepsis/*blood/*microbiology
MH  - Survival Analysis
MH  - Time Factors
MH  - beta-Glucans/*blood
PMC - PMC5531434
EDAT- 2017/07/28 06:00
MHDA- 2017/09/30 06:00
CRDT- 2017/07/28 06:00
PHST- 2017/02/23 00:00 [received]
PHST- 2017/06/30 00:00 [accepted]
PHST- 2017/07/28 06:00 [entrez]
PHST- 2017/07/28 06:00 [pubmed]
PHST- 2017/09/30 06:00 [medline]
AID - 10.1371/journal.pone.0181439 [doi]
AID - PONE-D-17-07329 [pii]
PST - epublish
SO  - PLoS One. 2017 Jul 27;12(7):e0181439. doi: 10.1371/journal.pone.0181439.
      eCollection 2017.