PMID- 28723967
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Epitope determination of immunogenic proteins of Neisseria gonorrhoeae.
PG  - e0180962
LID - 10.1371/journal.pone.0180962 [doi]
AB  - Neisseria gonorrhoeae is the causative organism of gonorrhoea, a sexually
      transmitted disease that globally accounts for an estimated 80 to 100 million new
      infections per year. Increasing resistances to all common antibiotics used for N.
      gonorrhoeae treatment pose the risk of an untreatable disease. Further knowledge 
      of ways of infection and host immune response are needed to understand the
      pathogen-host interaction and to discover new treatment alternatives against this
      disease. Therefore, detailed information about immunogenic proteins and their
      properties like epitope sites could advance further research in this area. In
      this work, we investigated immunogenic proteins of N. gonorrhoeae for linear
      epitopes by microarrays. Dominant linear epitopes were identified for eleven of
      the nineteen investigated proteins with three polyclonal rabbit antibodies from
      different immunisations. Identified linear epitopes were further examined for
      non-specific binding with antibodies to Escherichia coli and the closely related 
      pathogen Neisseria meningitidis. On top of that, amino acids crucial for the
      antibody epitope binding were detected by microarray based alanine scans.
FAU - Connor, Daniel O
AU  - Connor DO
AUID- ORCID: http://orcid.org/0000-0002-1313-0133
AD  - Department of Molecular and Cellular Bioanalytics, Fraunhofer Institute for Cell 
      Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany.
FAU - Danckert, Lena
AU  - Danckert L
AD  - Department of Molecular and Cellular Bioanalytics, Fraunhofer Institute for Cell 
      Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany.
FAU - Hoppe, Sebastian
AU  - Hoppe S
AD  - Department of Molecular and Cellular Bioanalytics, Fraunhofer Institute for Cell 
      Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany.
FAU - Bier, Frank F
AU  - Bier FF
AD  - Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany.
AD  - Department of Automation, Fraunhofer Institute for Cell Therapy and Immunology,
      Branch Bioanalytics and Bioprocesses, Potsdam, Germany.
FAU - von Nickisch-Rosenegk, Markus
AU  - von Nickisch-Rosenegk M
AD  - Department of Molecular and Cellular Bioanalytics, Fraunhofer Institute for Cell 
      Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany.
LA  - eng
PT  - Journal Article
DEP - 20170719
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bacterial Proteins)
RN  - 0 (Epitopes)
SB  - IM
MH  - Bacterial Proteins/*immunology
MH  - Epitopes/*immunology
MH  - Gonorrhea/*microbiology
MH  - Humans
MH  - Neisseria gonorrhoeae/*immunology
PMC - PMC5516995
EDAT- 2017/07/21 06:00
MHDA- 2017/09/28 06:00
CRDT- 2017/07/21 06:00
PHST- 2017/01/02 00:00 [received]
PHST- 2017/06/23 00:00 [accepted]
PHST- 2017/07/21 06:00 [entrez]
PHST- 2017/07/21 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
AID - 10.1371/journal.pone.0180962 [doi]
AID - PONE-D-17-00081 [pii]
PST - epublish
SO  - PLoS One. 2017 Jul 19;12(7):e0180962. doi: 10.1371/journal.pone.0180962.
      eCollection 2017.