PMID- 28696254
OWN - NLM
STAT- MEDLINE
DCOM- 20171016
LR  - 20180128
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 199
IP  - 4
DP  - 2017 Aug 15
TI  - Human Monocyte Subsets Are Transcriptionally and Functionally Altered in Aging in
      Response to Pattern Recognition Receptor Agonists.
PG  - 1405-1417
LID - 10.4049/jimmunol.1700148 [doi]
AB  - Age-related alterations in immunity have been linked to increased incidence of
      infections and decreased responses to vaccines in the aging population. Human
      peripheral blood monocytes are known to promote Ag presentation and antiviral
      activities; however, the impact of aging on monocyte functions remains an open
      question. We present an in-depth global analysis examining the impact of aging on
      classical (CD14(+)CD16(-)), intermediate (CD14(+)CD16(+)), and nonclassical
      (CD14(dim)CD16(+)) monocytes. Monocytes sorted from nonfrail healthy adults
      (21-40 y) and old (>/=65 y) individuals were analyzed after stimulation with
      TLR4, TLR7/8, and retinoic acid-inducible gene I agonists. Our data showed that
      under nonstimulated conditions, monocyte subsets did not reveal significant
      age-related alternations; however, agonist stimulated-monocytes from adults and
      old subjects did show differences at the transcriptional and functional levels.
      These alternations in many immune-related transcripts and biological processes
      resulted in reduced production of IFN-alpha, IFN-gamma, IL-1beta, CCL20, and
      CCL8, and higher expression of CX3CR1 in monocytes from old subjects. Our
      findings represent a comprehensive analysis of the influence of human aging on
      pattern recognition receptors signaling and monocyte functions, and have
      implications for strategies to enhance the immune response in the context of
      infection and immunization.
CI  - Copyright (c) 2017 by The American Association of Immunologists, Inc.
FAU - Metcalf, Talibah U
AU  - Metcalf TU
AD  - Division of Infectious Diseases and HIV Medicine, Department of Medicine, Drexel 
      University, Philadelphia, PA 19102.
FAU - Wilkinson, Peter A
AU  - Wilkinson PA
AD  - Department of Pathology, Case Western Reserve University, Cleveland, OH 44106.
FAU - Cameron, Mark J
AU  - Cameron MJ
AUID- ORCID: 0000-0003-4768-4094
AD  - Department of Epidemiology and Biostatistics, Case Western Reserve University,
      Cleveland, OH 44106.
FAU - Ghneim, Khader
AU  - Ghneim K
AD  - Department of Pathology, Case Western Reserve University, Cleveland, OH 44106.
FAU - Chiang, Cindy
AU  - Chiang C
AD  - Department of Microbiology, University of Chicago, Chicago, IL 60637.
FAU - Wertheimer, Anne M
AU  - Wertheimer AM
AUID- ORCID: 0000-0002-6627-4974
AD  - Department of Immunobiology, University of Arizona College of Medicine, Tucson,
      AZ 85724.
AD  - Arizona Center on Aging, University of Arizona College of Medicine, Tucson, AZ
      85724; and.
FAU - Hiscott, John B
AU  - Hiscott JB
AD  - Laboratorio Pasteur, Istituto Pasteur-Fondazione Cenci Bolognetti, 00161 Rome,
      Italy.
FAU - Nikolich-Zugich, Janko
AU  - Nikolich-Zugich J
AD  - Department of Immunobiology, University of Arizona College of Medicine, Tucson,
      AZ 85724.
AD  - Arizona Center on Aging, University of Arizona College of Medicine, Tucson, AZ
      85724; and.
FAU - Haddad, Elias K
AU  - Haddad EK
AD  - Division of Infectious Diseases and HIV Medicine, Department of Medicine, Drexel 
      University, Philadelphia, PA 19102; elias.elhaddad@drexelmed.edu.
LA  - eng
GR  - HHSN272201100017C/AI/NIAID NIH HHS/United States
GR  - HHSN272201400055C/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170710
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytokines)
RN  - 0 (FCGR3B protein, human)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Receptors, IgG)
RN  - 0 (Receptors, Pattern Recognition)
RN  - 0 (TLR4 protein, human)
RN  - 0 (TLR7 protein, human)
RN  - 0 (TLR8 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptor 7)
RN  - 0 (Toll-Like Receptor 8)
RN  - 9008-11-1 (Interferons)
SB  - AIM
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aging/*immunology
MH  - Cytokines/*biosynthesis/genetics/immunology
MH  - Female
MH  - GPI-Linked Proteins/analysis
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunity, Innate
MH  - Interferons/biosynthesis/immunology
MH  - Lipopolysaccharide Receptors/analysis
MH  - Male
MH  - Middle Aged
MH  - Monocytes/classification/*immunology/*physiology
MH  - Receptors, IgG/analysis
MH  - Receptors, Pattern Recognition/*agonists/genetics/*metabolism
MH  - Toll-Like Receptor 4/agonists/immunology/metabolism
MH  - Toll-Like Receptor 7/agonists/immunology/metabolism
MH  - Toll-Like Receptor 8/agonists/immunology/metabolism
MH  - *Transcription, Genetic
MH  - Young Adult
PMC - PMC5548610
MID - NIHMS885579
EDAT- 2017/07/12 06:00
MHDA- 2017/10/17 06:00
CRDT- 2017/07/12 06:00
PMCR- 2018/08/15 00:00
PHST- 2017/01/30 00:00 [received]
PHST- 2017/06/08 00:00 [accepted]
PHST- 2018/08/15 00:00 [pmc-release]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/10/17 06:00 [medline]
PHST- 2017/07/12 06:00 [entrez]
AID - jimmunol.1700148 [pii]
AID - 10.4049/jimmunol.1700148 [doi]
PST - ppublish
SO  - J Immunol. 2017 Aug 15;199(4):1405-1417. doi: 10.4049/jimmunol.1700148. Epub 2017
      Jul 10.