PMID- 28692534
DCOM- 20180508
LR  - 20180523
IS  - 1473-5571 (Electronic)
IS  - 0269-9370 (Linking)
VI  - 31
IP  - 14
DP  - 2017 Sep 10
TI  - Blood neuron-derived exosomes as biomarkers of cognitive impairment in HIV.
PG  - F9-F17
LID - 10.1097/QAD.0000000000001595 [doi]
AB  - OBJECTIVE: To investigate proteins associated with neuronal damage in plasma
      neuron-derived exosomes (NDE) of HIV-infected study participants as a liquid
      biomarker for cognitive impairment. METHODS: Plasma NDE were isolated using
      precipitation and immunoadsorption with antibody to a cell surface-specific
      neuronal marker. Total exosomes and NDE were enumerated, characterized, and
      proteins extracted and targets quantified by ELISA. RESULTS: Plasma NDE from 23
      HIV seropositive individuals of which 11 had mild cognitive impairment, and 12
      HIV seronegative controls of which three had cognitive impairment were isolated. 
      NDE were enriched for the neuronal markers neurofilament light (NF-L) and
      synaptophysin (SYP). Neuropsychologically impaired individuals had fewer NDE
      compared with neuropsychologically normal study participants. NDE from
      neuropsychologically impaired study participants had significantly higher levels 
      of high-mobility group box 1 (HMGB1), NF-L, and amyloid beta proteins compared
      with neuropsychologically normal individuals. NDE HMGB1 protein significantly
      decreased with age in HIV-infected individuals. CONCLUSION: Plasma NDE were
      altered in several ways in HIV infection. Elevated HMGB1, NF-L, and amyloid beta 
      proteins could distinguish cognitive impairment. NDE contents reflect neuronal
      health in 'real time' and may be useful for following cognitive impairment and
      response to therapy in HIV infection.
FAU - Sun, Bing
AU  - Sun B
AD  - aVeterans Affairs Medical Center bDepartments of Laboratory Medicine and
      Medicine, University of California, San Francisco, California, USA.
FAU - Dalvi, Pranjali
AU  - Dalvi P
FAU - Abadjian, Linda
AU  - Abadjian L
FAU - Tang, Norina
AU  - Tang N
FAU - Pulliam, Lynn
AU  - Pulliam L
LA  - eng
GR  - R01 MH085538/MH/NIMH NIH HHS/United States
GR  - R21 MH112483/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Biomarkers)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (neurofilament protein L)
SB  - IM
SB  - X
MH  - AIDS Dementia Complex/*diagnosis/pathology
MH  - Adult
MH  - Aged
MH  - Amyloid beta-Peptides/*analysis
MH  - Biomarkers/blood
MH  - *Blood Chemical Analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exosomes/*chemistry
MH  - HIV Infections/*complications
MH  - HMGB1 Protein/*analysis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neurofilament Proteins/*analysis
PMC - PMC5578870
MID - NIHMS892034
EDAT- 2017/07/12 06:00
MHDA- 2018/05/09 06:00
CRDT- 2017/07/11 06:00
PMCR- 2018/09/10 00:00
PHST- 2018/09/10 00:00 [pmc-release]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2018/05/09 06:00 [medline]
PHST- 2017/07/11 06:00 [entrez]
AID - 10.1097/QAD.0000000000001595 [doi]
PST - ppublish
SO  - AIDS. 2017 Sep 10;31(14):F9-F17. doi: 10.1097/QAD.0000000000001595.