PMID- 28622339
OWN - NLM
STAT- MEDLINE
DCOM- 20170913
LR  - 20170913
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 6
DP  - 2017
TI  - The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144
      are secreted into the lumen of the inclusion.
PG  - e0178856
LID - 10.1371/journal.pone.0178856 [doi]
AB  - Chlamydia trachomatis is a human bacterial pathogen causing ocular and genital
      infections. It multiplies exclusively within an intracellular membrane-bound
      vacuole, the inclusion, and uses a type III secretion system to manipulate host
      cells by injecting them with bacterially-encoded effector proteins. In this work,
      we characterized the expression and subcellular localization in infected host
      cells of the C. trachomatis CT142, CT143, and CT144 proteins, which we previously
      showed to be type III secretion substrates. Transcriptional analyses in C.
      trachomatis confirmed the prediction that ct142, ct143 and ct144 are organized in
      an operon and revealed that their expression is likely driven by the main sigma
      factor, sigma66. In host cells infected by C. trachomatis, production of CT142
      and CT143 could be detected by immunoblotting from 20-26 h post-infection.
      Immunofluorescence microscopy of infected cells revealed that from 20 h
      post-infection CT143 appeared mostly as globular structures outside of the
      bacterial cells but within the lumen of the inclusion. Furthermore,
      immunofluorescence microscopy of cells infected by C. trachomatis strains
      carrying plasmids producing CT142, CT143, or CT144 under the control of the ct142
      promoter and with a C-terminal double hemagglutinin (2HA) epitope tag revealed
      that CT142-2HA, CT143-2HA or CT144-2HA showed an identical localization to
      chromosomally-encoded CT143. Moreover, CT142-2HA or CT144-2HA and CT143 produced 
      by the same bacteria co-localized in the lumen of the inclusion. Overall, these
      data suggest that the CT142, CT143, and CT144 type III secretion substrates are
      secreted into the lumen of the inclusion where they might form a protein complex.
FAU - da Cunha, Maria
AU  - da Cunha M
AD  - UCIBIO-REQUIMTE, Departamento de Ciencias da Vida, Faculdade de Ciencias e
      Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
AD  - Instituto de Tecnologia Quimica e Biologica Antonio Xavier, Universidade Nova de 
      Lisboa, Oeiras, Portugal.
FAU - Pais, Sara V
AU  - Pais SV
AD  - UCIBIO-REQUIMTE, Departamento de Ciencias da Vida, Faculdade de Ciencias e
      Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
FAU - Bugalhao, Joana N
AU  - Bugalhao JN
AD  - UCIBIO-REQUIMTE, Departamento de Ciencias da Vida, Faculdade de Ciencias e
      Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
FAU - Mota, Luis Jaime
AU  - Mota LJ
AUID- ORCID: http://orcid.org/0000-0002-5592-8397
AD  - UCIBIO-REQUIMTE, Departamento de Ciencias da Vida, Faculdade de Ciencias e
      Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
AD  - Instituto de Tecnologia Quimica e Biologica Antonio Xavier, Universidade Nova de 
      Lisboa, Oeiras, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20170616
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Secretion Systems)
SB  - IM
MH  - Animals
MH  - *Bacterial Proteins/genetics/secretion
MH  - *Bacterial Secretion Systems/genetics/secretion
MH  - Cercopithecus aethiops
MH  - *Chlamydia trachomatis/genetics/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - *Plasmids/genetics/metabolism
MH  - Vero Cells
PMC - PMC5473537
EDAT- 2017/06/18 06:00
MHDA- 2017/09/14 06:00
CRDT- 2017/06/17 06:00
PHST- 2017/04/15 00:00 [received]
PHST- 2017/05/21 00:00 [accepted]
PHST- 2017/06/17 06:00 [entrez]
PHST- 2017/06/18 06:00 [pubmed]
PHST- 2017/09/14 06:00 [medline]
AID - 10.1371/journal.pone.0178856 [doi]
AID - PONE-D-17-14708 [pii]
PST - epublish
SO  - PLoS One. 2017 Jun 16;12(6):e0178856. doi: 10.1371/journal.pone.0178856.
      eCollection 2017.