PMID- 28601248
OWN - NLM
STAT- MEDLINE
DCOM- 20180808
LR  - 20180808
IS  - 1879-0267 (Electronic)
IS  - 0020-1383 (Linking)
VI  - 48
IP  - 8
DP  - 2017 Aug
TI  - Evaluation of bone turnover markers and serum minerals variations for predicting 
      fracture healing versus non-union processes in adult sheep as a model for
      orthopedic research.
PG  - 1768-1775
LID - S0020-1383(17)30350-9 [pii]
LID - 10.1016/j.injury.2017.05.025 [doi]
AB  - Bone turnover markers (BTMs) have been considered as an auxiliary method of
      following the fracture healing process and for early prediction of impaired bone 
      healing. A better understanding of the potential of BTMs in this application
      could allow for earlier interventions and improved patient care. The aim of this 
      study with a large animal experimental model was to assess the variation of bone 
      formation markers - namely the total alkaline phosphatase (ALP) and its
      bone-specific isoform (BALP), serum concentration of intact osteocalcin (OC),
      N-terminal propeptide type III procollagen (PIIINP) and of bone resorption
      markers - namely tartrate resistant acid phosphatase (TRAP) and deoxypyridinoline
      crosslink (DPD) during the first stages of a normal fracture healing process and 
      of a segmental critical size defect (CSD), which progresses to a non-union
      process. Thirty healthy female sheep (Portuguese Churra-da-Terra-Quente breed),
      approximately 4-years-old, were enrolled in this study. Jugular venous blood
      samples were collected pre-operatively and at 1, 2, 3, 4, 6, 8, 10 and 12
      post-operative weeks. The animals of the CSD group showed significant lower serum
      levels of BALP, OC and significant higher serum PIIINP levels at early stages of 
      the fracture healing process, compared with animals that progressed in a normal
      fracture healing process. Serum BALP, OC and PIIINP levels could be useful as
      non-invasive auxiliary tools with other complementary methods for predicting the 
      outcome of traumatic bone fractures.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Sousa, Cristina P
AU  - Sousa CP
AD  - Center Hospitalar of Porto, Largo Prof. Abel Salazar 4099-001 Porto, Portugal.
      Electronic address: cristinamariapsousa@gmail.com.
FAU - Lopez-Pena, Monica
AU  - Lopez-Pena M
AD  - Department of Veterinary Clinics Sciences, Faculty of Veterinary Medicine,
      University of Santiago de Compostela, University Campus, Av. Carballo Calero,
      27002 Lugo, Spain.
FAU - Guzon, Fernando M
AU  - Guzon FM
AD  - Department of Veterinary Clinics Sciences, Faculty of Veterinary Medicine,
      University of Santiago de Compostela, University Campus, Av. Carballo Calero,
      27002 Lugo, Spain.
FAU - Abreu, Humberto V De
AU  - Abreu HV
AD  - Department of Veterinary Sciences, Agricultural and Veterinary Sciences School
      (ECAV), University of Tras-os-Montes and Alto Douro (UTAD), Quinta de Prados,
      5000-801 Vila Real, Portugal.
FAU - Luis, Maurino R
AU  - Luis MR
AD  - Department of Veterinary Sciences, Agricultural and Veterinary Sciences School
      (ECAV), University of Tras-os-Montes and Alto Douro (UTAD), Quinta de Prados,
      5000-801 Vila Real, Portugal.
FAU - Viegas, Carlos A
AU  - Viegas CA
AD  - Department of Veterinary Sciences, Agricultural and Veterinary Sciences School
      (ECAV), University of Tras-os-Montes and Alto Douro (UTAD), Quinta de Prados,
      5000-801 Vila Real, Portugal; CITAB - Centre for the Research and Technology of
      Agro-Environmental and Biological Sciences, UTAD, Vila Real, Portugal.
FAU - Camassa, Jose
AU  - Camassa J
AD  - Department of Veterinary Sciences, Agricultural and Veterinary Sciences School
      (ECAV), University of Tras-os-Montes and Alto Douro (UTAD), Quinta de Prados,
      5000-801 Vila Real, Portugal.
FAU - Azevedo, Jorge T De
AU  - Azevedo JT
AD  - Department of Animal Sciences, ECAV, UTAD, Vila Real, Portugal; CECAV - Centre
      for Animal Sciences and Veterinary Studies, UTAD, Vila Real, Portugal.
FAU - Cabrita, Antonio S
AU  - Cabrita AS
AD  - Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 
      Rua larga, 3004-504 Coimbra, Portugal.
FAU - Reis, Rui L
AU  - Reis RL
AD  - 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of
      Minho, Headquarters of the European Institute of Excellence on Tissue Engineering
      and Regenerative Medicine, AvePark, 4805-017 Barco, Guimaraes, Portugal;
      ICVS/3B's - PT Government Associate Laboratory, Braga/Guimaraes, Portugal.
FAU - Gomes, Manuela E
AU  - Gomes ME
AD  - 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of
      Minho, Headquarters of the European Institute of Excellence on Tissue Engineering
      and Regenerative Medicine, AvePark, 4805-017 Barco, Guimaraes, Portugal;
      ICVS/3B's - PT Government Associate Laboratory, Braga/Guimaraes, Portugal.
FAU - Dias, Isabel R
AU  - Dias IR
AD  - Department of Veterinary Sciences, Agricultural and Veterinary Sciences School
      (ECAV), University of Tras-os-Montes and Alto Douro (UTAD), Quinta de Prados,
      5000-801 Vila Real, Portugal; CITAB - Centre for the Research and Technology of
      Agro-Environmental and Biological Sciences, UTAD, Vila Real, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20170517
PL  - Netherlands
TA  - Injury
JT  - Injury
JID - 0226040
RN  - 0 (Amino Acids)
RN  - 0 (Biomarkers)
RN  - 0 (Minerals)
RN  - 0 (Peptide Fragments)
RN  - 0 (Procollagen)
RN  - 0 (procollagen Type III-N-terminal peptide)
RN  - 104982-03-8 (Osteocalcin)
RN  - 90032-33-0 (deoxypyridinoline)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/blood
MH  - Amino Acids
MH  - Animals
MH  - Biomarkers/blood
MH  - *Biomedical Research
MH  - Bone Remodeling/*physiology
MH  - Female
MH  - Fracture Healing/*physiology
MH  - Fractures, Bone/*blood/physiopathology
MH  - Fractures, Ununited/*blood/physiopathology
MH  - Minerals/*blood
MH  - Models, Animal
MH  - *Orthopedics
MH  - Osteocalcin/blood
MH  - Peptide Fragments
MH  - Predictive Value of Tests
MH  - Procollagen
MH  - Sheep/blood
MH  - Tartrate-Resistant Acid Phosphatase
OTO - NOTNLM
OT  - Bone turnover markers
OT  - Critical size defect
OT  - Delayed union
OT  - Fracture healing process
OT  - Non-union
OT  - Sheep
EDAT- 2017/06/12 06:00
MHDA- 2018/08/09 06:00
CRDT- 2017/06/12 06:00
PHST- 2016/11/04 00:00 [received]
PHST- 2017/05/09 00:00 [revised]
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/06/12 06:00 [pubmed]
PHST- 2018/08/09 06:00 [medline]
PHST- 2017/06/12 06:00 [entrez]
AID - S0020-1383(17)30350-9 [pii]
AID - 10.1016/j.injury.2017.05.025 [doi]
PST - ppublish
SO  - Injury. 2017 Aug;48(8):1768-1775. doi: 10.1016/j.injury.2017.05.025. Epub 2017
      May 17.