PMID- 28570663
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 6
DP  - 2017
TI  - Rectal administration of a chlamydial subunit vaccine protects against genital
      infection and upper reproductive tract pathology in mice.
PG  - e0178537
LID - 10.1371/journal.pone.0178537 [doi]
AB  - In this study, we tested the hypothesis that rectal immunization with a VCG-based
      chlamydial vaccine would cross-protect mice against heterologous genital
      Chlamydia trachomatis infection and Chlamydia-induced upper genital tract
      pathologies in mice. Female mice were immunized with a C. trachomatis serovar
      D-derived subunit vaccine or control or live serovar D elementary bodies (EBs)
      and the antigen-specific mucosal and systemic immune responses were
      characterized. Vaccine efficacy was determined by evaluating the intensity and
      duration of genital chlamydial shedding following intravaginal challenge with
      live serovar E chlamydiae. Protection against upper genital tract pathology was
      determined by assessing infertility and tubal inflammation. Rectal immunization
      elicited high levels of chlamydial-specific IFN-gamma-producing CD4 T cells and
      humoral immune responses in mucosal and systemic tissues. The elicited immune
      effectors cross-reacted with the serovar E chlamydial antigen and reduced the
      length and intensity of genital chlamydial shedding. Furthermore, immunization
      with the VCG-vaccine but not the rVCG-gD2 control reduced the incidence of tubal 
      inflammation and protected mice against Chlamydia-induced infertility. These
      results highlight the potential of rectal immunization as a viable mucosal route 
      for inducing protective immunity in the female genital tract.
FAU - Pais, Roshan
AU  - Pais R
AD  - Department of Microbiology, Biochemistry and Immunology, Morehouse School of
      Medicine, Atlanta, Georgia, United States of America.
FAU - Omosun, Yusuf
AU  - Omosun Y
AD  - Department of Microbiology, Biochemistry and Immunology, Morehouse School of
      Medicine, Atlanta, Georgia, United States of America.
FAU - He, Qing
AU  - He Q
AD  - Department of Microbiology, Biochemistry and Immunology, Morehouse School of
      Medicine, Atlanta, Georgia, United States of America.
FAU - Blas-Machado, Uriel
AU  - Blas-Machado U
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, The University 
      of Georgia, Athens, Georgia, United States of America.
FAU - Black, Carolyn
AU  - Black C
AD  - Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, United States
      of America.
FAU - Igietseme, Joseph U
AU  - Igietseme JU
AD  - Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, United States
      of America.
FAU - Fujihashi, Kohtaro
AU  - Fujihashi K
AD  - Department of Pediatric Dentistry, Immunobiology Vaccine Center, The University
      of Alabama at Birmingham, Birmingham Alabama, United States of America.
FAU - Eko, Francis O
AU  - Eko FO
AD  - Department of Microbiology, Biochemistry and Immunology, Morehouse School of
      Medicine, Atlanta, Georgia, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20170601
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Vaccines)
SB  - IM
MH  - Administration, Rectal
MH  - Animals
MH  - Antibodies, Bacterial/biosynthesis
MH  - Bacterial Vaccines/*administration & dosage
MH  - Chlamydia Infections/immunology/*prevention & control
MH  - Chlamydia trachomatis/*immunology
MH  - Female
MH  - Immunity, Cellular
MH  - Mice
MH  - Mice, Inbred C57BL
PMC - PMC5453548
EDAT- 2017/06/02 06:00
MHDA- 2017/09/28 06:00
CRDT- 2017/06/02 06:00
PHST- 2016/10/11 00:00 [received]
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/06/02 06:00 [entrez]
PHST- 2017/06/02 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
AID - 10.1371/journal.pone.0178537 [doi]
AID - PONE-D-16-40582 [pii]
PST - epublish
SO  - PLoS One. 2017 Jun 1;12(6):e0178537. doi: 10.1371/journal.pone.0178537.
      eCollection 2017.