PMID- 28542601
OWN - NLM
STAT- MEDLINE
DCOM- 20170911
LR  - 20190208
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 5
DP  - 2017
TI  - The epidemiology of Staphylococcus aureus carriage in patients attending inner
      city sexually transmitted infections and community clinics in Calgary, Canada.
PG  - e0178557
LID - 10.1371/journal.pone.0178557 [doi]
AB  - BACKGROUND: Although the nares represent the most common carriage site for
      traditional hospital-associated strains of Staphylococcus aureus (SA), the
      predominant site of carriage of SA in the community is less certain. METHODS: We 
      conducted a cross-sectional study in 285 patients attending sexually transmitted 
      diseases and inner-city clinics to evaluate the prevalence, body site
      colonisation and risk factors associated with carriage of methicillin susceptible
      SA (MSSA). All isolates were characterized by pulsed field gel electrophoresis,
      staphylococcal cassette chromosome mec, staphylococcal protein A and multilocus
      sequence typing. RESULTS: The prevalence of colonisation with SA was 57.5%
      (164/285); 162 (56.8%) participants were colonized with MSSA, and 4 (1.4%) with
      methicillin-resistant SA (MRSA), 2 of them were co-colonised with both MRSA and
      MSSA. The most common sites of colonisation were the throat (73.1%), nares
      (65.2%) and interdigital web spaces of the hand (21.3%). Three out of 4 MRSA
      isolates were USA300-MRSA strains. Twelve MSSA isolates were closely related to
      the USA300 CA-MRSA. We identified sexual behaviours such as having more than 6
      heterosexual sexual partners in the last 6 months and trimming pubic hair to be
      independently associated with MSSA colonisation, and more specifically practicing
      oral sex as a risk factor for throat colonisation. CONCLUSION: There is a high
      prevalence of MSSA carriage in this population, with a low prevalence of MRSA.
      The throat was the most common site of carriage and sexual behaviours were found 
      to be risk factors for MSSA colonisation. Close strain relatedness of MSSA and
      USA300-MRSA isolates suggests either gain or loss of the SCCmec element,
      respectively.
FAU - Ugarte Torres, Alejandra
AU  - Ugarte Torres A
AUID- ORCID: http://orcid.org/0000-0002-8872-5158
AD  - Division of Infectious Diseases, Department of Medicine, University of Calgary
      and Alberta Health Services, Calgary, Alberta, Canada.
FAU - Chu, Angel
AU  - Chu A
AD  - Division of Infectious Diseases, Department of Medicine, University of Calgary
      and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Sexually Transmitted Infections Clinic, Alberta Health Services, Calgary,
      Alberta, Canada.
FAU - Read, Ron
AU  - Read R
AD  - Division of Infectious Diseases, Department of Medicine, University of Calgary
      and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Sexually Transmitted Infections Clinic, Alberta Health Services, Calgary,
      Alberta, Canada.
AD  - Department of Microbiology, Infectious Diseases and Immunology, Cumming School of
      Medicine, Calgary, Alberta, Canada.
AD  - Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of 
      Calgary and Alberta Health Services, Calgary, Alberta, Canada.
FAU - MacDonald, Judy
AU  - MacDonald J
AD  - Department of Community Health Sciences, Cumming School of Medicine, University
      of Calgary, Calgary, Alberta, Canada.
AD  - Infection Prevention & Control, Alberta Health Services, Calgary, Alberta,
      Canada.
FAU - Gregson, Daniel
AU  - Gregson D
AD  - Division of Infectious Diseases, Department of Medicine, University of Calgary
      and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of 
      Calgary and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Department of Pathology and Laboratory Medicine, Cumming School of Medicine and
      Alberta Health Services, Calgary, Alberta, Canada.
AD  - O'Brien Institute for Public Health, Cumming School of Medicine and Alberta
      Health Services, University of Calgary, Calgary, Alberta, Canada.
FAU - Louie, Thomas
AU  - Louie T
AD  - Division of Infectious Diseases, Department of Medicine, University of Calgary
      and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Department of Microbiology, Infectious Diseases and Immunology, Cumming School of
      Medicine, Calgary, Alberta, Canada.
AD  - Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of 
      Calgary and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Infection Prevention & Control, Alberta Health Services, Calgary, Alberta,
      Canada.
FAU - Delongchamp, Johanna
AU  - Delongchamp J
AD  - Centre for Antimicrobial Resistance, Alberta Health Services and University of
      Calgary, Alberta, Canada.
FAU - Ward, Linda
AU  - Ward L
AD  - Infection Prevention & Control, Alberta Health Services, Calgary, Alberta,
      Canada.
FAU - McClure, Joann
AU  - McClure J
AD  - Centre for Antimicrobial Resistance, Alberta Health Services and University of
      Calgary, Alberta, Canada.
FAU - Zhang, Kunyan
AU  - Zhang K
AD  - Division of Infectious Diseases, Department of Medicine, University of Calgary
      and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Department of Microbiology, Infectious Diseases and Immunology, Cumming School of
      Medicine, Calgary, Alberta, Canada.
AD  - Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of 
      Calgary and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Department of Pathology and Laboratory Medicine, Cumming School of Medicine and
      Alberta Health Services, Calgary, Alberta, Canada.
AD  - Centre for Antimicrobial Resistance, Alberta Health Services and University of
      Calgary, Alberta, Canada.
AD  - Calgary Laboratory Services, Alberta Health Services, Calgary, Alberta, Canada.
FAU - Conly, John
AU  - Conly J
AD  - Division of Infectious Diseases, Department of Medicine, University of Calgary
      and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Department of Microbiology, Infectious Diseases and Immunology, Cumming School of
      Medicine, Calgary, Alberta, Canada.
AD  - Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of 
      Calgary and Alberta Health Services, Calgary, Alberta, Canada.
AD  - Department of Pathology and Laboratory Medicine, Cumming School of Medicine and
      Alberta Health Services, Calgary, Alberta, Canada.
AD  - O'Brien Institute for Public Health, Cumming School of Medicine and Alberta
      Health Services, University of Calgary, Calgary, Alberta, Canada.
AD  - Centre for Antimicrobial Resistance, Alberta Health Services and University of
      Calgary, Alberta, Canada.
AD  - Calgary Laboratory Services, Alberta Health Services, Calgary, Alberta, Canada.
LA  - eng
PT  - Journal Article
DEP - 20170525
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Canada
MH  - Community-Acquired Infections/*microbiology
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Male
MH  - Methicillin Resistance/genetics
MH  - Methicillin-Resistant Staphylococcus aureus/genetics/pathogenicity
MH  - Microbial Sensitivity Tests/methods
MH  - Middle Aged
MH  - Molecular Epidemiology/methods
MH  - Molecular Typing
MH  - Sexually Transmitted Diseases/*embryology/*microbiology
MH  - Staphylococcal Infections/*epidemiology/*microbiology
MH  - Staphylococcus aureus/*genetics/pathogenicity
MH  - Virulence Factors/genetics
MH  - Young Adult
PMC - PMC5444836
EDAT- 2017/05/26 06:00
MHDA- 2017/09/12 06:00
CRDT- 2017/05/26 06:00
PHST- 2016/11/23 00:00 [received]
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/05/26 06:00 [entrez]
PHST- 2017/05/26 06:00 [pubmed]
PHST- 2017/09/12 06:00 [medline]
AID - 10.1371/journal.pone.0178557 [doi]
AID - PONE-D-16-46580 [pii]
PST - epublish
SO  - PLoS One. 2017 May 25;12(5):e0178557. doi: 10.1371/journal.pone.0178557.
      eCollection 2017.