PMID- 28511643
OWN - NLM
STAT- MEDLINE
DCOM- 20170929
LR  - 20170929
IS  - 1471-2415 (Electronic)
IS  - 1471-2415 (Linking)
VI  - 17
IP  - 1
DP  - 2017 May 16
TI  - Long noncoding RNA expression profile in HLE B-3 cells during TGF-beta2-induced
      epithelial-mesenchymal transition.
PG  - 69
LID - 10.1186/s12886-017-0461-z [doi]
AB  - BACKGROUND: Recent evidence has shown that long noncoding RNAs (lncRNAs) are
      involved in the process of epithelial-mesenchymal transition (EMT). However,
      little research has focused on the expression profile of lncRNAs during EMT in
      human lens epithelial cells (LECs) and their functions have not yet been
      described. METHODS: Dysregulated lncRNAs and mRNAs in normal human lens
      epithelial B-3(HLE B-3) cells and during transforming growth factor
      beta2(TGF-beta2)-induced EMT were analyzed via lncRNA microarray. Gene Ontology
      (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analyses of
      differentially expressed mRNAs were performed to identify their functions and
      pathologic pathways. Six candidate lncRNAs were validated via quantitative
      real-time reverse transcription polymerase chain reaction(qRT-PCR) to confirm the
      microarray data. RESULTS: A total of 775 lncRNAs (325 up-regulated and 450
      down-regulated) and 935 mRNAs (329 up-regulated and 606 down-regulated) were
      differentially expressed in HLE B-3 cells during TGF-beta2-induced EMT compared
      to normal HLE B-3 cells. GO and KEGG Pathway analyses indicated the functions of 
      differentially expressed mRNAs in the TGF-beta2-induced EMT in HLE B-3 cells.
      qRT-PCR confirmed the trends indicated in microarray analysis for all 6 candidate
      lncRNAs. CONCLUSION: Our study lays the foundation for future research in lncRNAs
      related to EMT in HLE B-3 cells and could provide new avenues for the prevention 
      and treatment of posterior capsule opacification (PCO).
FAU - Zhang, Bingyu
AU  - Zhang B
AD  - Guilin Medical University, Guangxi Zhuang Autonomous Region, Guilin, 541000,
      China.
FAU - Chen, Yang
AU  - Chen Y
AD  - Guilin Medical University, Guangxi Zhuang Autonomous Region, Guilin, 541000,
      China.
FAU - Qiu, Meiyuan
AU  - Qiu M
AD  - Department of Ophthalmology, Guilin Medical University Affiliated Hospital,
      Guangxi Zhuang Autonomous Region, Guilin, 541001, China.
FAU - Ding, Zhixiang
AU  - Ding Z
AUID- ORCID: http://orcid.org/0000-0001-8573-8730
AD  - Department of Ophthalmology, Guilin Medical University Affiliated Hospital,
      Guangxi Zhuang Autonomous Region, Guilin, 541001, China. zxding99@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170516
PL  - England
TA  - BMC Ophthalmol
JT  - BMC ophthalmology
JID - 100967802
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta2)
SB  - IM
MH  - Blotting, Western
MH  - Capsule Opacification/*genetics/metabolism/pathology
MH  - Cell Line
MH  - Epithelial Cells/drug effects/*metabolism/pathology
MH  - Epithelial-Mesenchymal Transition/drug effects/*genetics
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Microarray Analysis
MH  - RNA, Long Noncoding/biosynthesis/*genetics
MH  - RNA, Messenger/*genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Transforming Growth Factor beta2/*pharmacology
PMC - PMC5434530
OTO - NOTNLM
OT  - Epithelial-mesenchymal transition
OT  - HLE B-3 cells
OT  - LncRNA
OT  - Microarray
OT  - Posterior capsule opacification
EDAT- 2017/05/18 06:00
MHDA- 2017/09/30 06:00
CRDT- 2017/05/18 06:00
PHST- 2016/10/17 00:00 [received]
PHST- 2017/05/08 00:00 [accepted]
PHST- 2017/05/18 06:00 [entrez]
PHST- 2017/05/18 06:00 [pubmed]
PHST- 2017/09/30 06:00 [medline]
AID - 10.1186/s12886-017-0461-z [doi]
AID - 10.1186/s12886-017-0461-z [pii]
PST - epublish
SO  - BMC Ophthalmol. 2017 May 16;17(1):69. doi: 10.1186/s12886-017-0461-z.