PMID- 28494018
OWN - NLM
STAT- MEDLINE
DCOM- 20170911
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 5
DP  - 2017
TI  - Pathogenic outcome following experimental infection of sheep with Chlamydia
      abortus variant strains LLG and POS.
PG  - e0177653
LID - 10.1371/journal.pone.0177653 [doi]
AB  - This study investigated the pathogenesis of two variant strains (LLG and POS) of 
      Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is
      known to be responsible for late term abortion in small ruminants. Challenge with
      the three strains at mid-gestation resulted in similar pregnancy outcomes, with
      abortion occurring in approximately 50-60% of ewes with the mean gestational
      lengths also being similar. However, differences were observed in the severity of
      placental pathology, with infection appearing milder for strain LLG, which was
      reflected in the lower number of organisms shed in vaginal swabs post-partum and 
      less gross pathology and organisms present in placental smears. Results for
      strain POS were somewhat different than LLG with a more focal restriction of
      infection observed. Post-abortion antibody responses revealed prominent
      differences in seropositivity to the major outer membrane protein (MOMP) present 
      in elementary body (EB) preparations under denaturing conditions, most notably
      with anti-LLG and anti-POS convalescent sera where there was no or reduced
      detection of MOMP present in EBs derived from the three strains. These results
      and additional analysis of whole EB and chlamydial outer membrane complex
      preparations suggest that there are conformational differences in MOMP for the
      three strains. Overall, the results suggest that gross placental pathology and
      clinical outcome is not indicative of bacterial colonization and the severity of 
      infection. The results also highlight potential conformational differences in
      MOMP epitopes that perhaps impact on disease diagnosis and the development of new
      vaccines.
FAU - Livingstone, Morag
AU  - Livingstone M
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Wheelhouse, Nicholas
AU  - Wheelhouse N
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Ensor, Hannah
AU  - Ensor H
AD  - Biomathematics and Statistics Scotland, Edinburgh, United Kingdom.
FAU - Rocchi, Mara
AU  - Rocchi M
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Maley, Stephen
AU  - Maley S
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Aitchison, Kevin
AU  - Aitchison K
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Wattegedera, Sean
AU  - Wattegedera S
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Wilson, Kim
AU  - Wilson K
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Sait, Michelle
AU  - Sait M
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Siarkou, Victoria
AU  - Siarkou V
AD  - Laboratory of Microbiology and Infectious Diseases, School of Veterinary
      Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki,
      Thessaloniki, Greece.
FAU - Vretou, Evangelia
AU  - Vretou E
AD  - Formerly Laboratory of Biotechnology, Department of Microbiology, Hellenic
      Pasteur Institute, Athens, Greece.
FAU - Entrican, Gary
AU  - Entrican G
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Dagleish, Mark
AU  - Dagleish M
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
FAU - Longbottom, David
AU  - Longbottom D
AUID- ORCID: http://orcid.org/0000-0003-0086-8443
AD  - Moredun Research Institute, Penicuik, Midlothian, United Kingdom.
LA  - eng
PT  - Journal Article
DEP - 20170511
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Bacterial)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - Chlamydia/immunology/*physiology
MH  - Chlamydia Infections/microbiology/pathology/*veterinary
MH  - Female
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Placenta/microbiology/pathology
MH  - Pregnancy
MH  - Sheep/*microbiology
MH  - Sheep Diseases/*microbiology/*pathology
MH  - Treatment Outcome
MH  - Vagina/microbiology
PMC - PMC5426687
EDAT- 2017/05/12 06:00
MHDA- 2017/09/12 06:00
CRDT- 2017/05/12 06:00
PHST- 2017/02/02 00:00 [received]
PHST- 2017/05/01 00:00 [accepted]
PHST- 2017/05/12 06:00 [entrez]
PHST- 2017/05/12 06:00 [pubmed]
PHST- 2017/09/12 06:00 [medline]
AID - 10.1371/journal.pone.0177653 [doi]
AID - PONE-D-17-04304 [pii]
PST - epublish
SO  - PLoS One. 2017 May 11;12(5):e0177653. doi: 10.1371/journal.pone.0177653.
      eCollection 2017.