PMID- 28472126
OWN - NLM
STAT- MEDLINE
DCOM- 20170915
LR  - 20170915
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 5
DP  - 2017
TI  - Growth and adherence of Staphylococcus aureus were enhanced through the PGE2
      produced by the activated COX-2/PGE2 pathway of infected oral epithelial cells.
PG  - e0177166
LID - 10.1371/journal.pone.0177166 [doi]
AB  - Staphylococcus aureus is a major pathogen of varieties of oral mucous infection. 
      Prostaglandin E2 (PGE2) is a pro-inflammatory factor and Cyclooxygenase 2 (COX-2)
      is a critical enzyme of PGE2 biosynthesis. The purpose of this study is to
      investigate whether Staphylococcus aureus can increase PGE2 production of oral
      epithelial cells and how PGE2 functions in the growth and adherence of
      Staphylococcus aureus. mRNA levels of COX-2, fnbpA and fnbpB were estimated by
      quantitative PCR. PGE2 production was measured by Enzyme Linked Immunosorbent
      Assay (ELISA). The binding biomass of Staphylococcus aureus to human fibronectin 
      was investigated by crystal violet staining and confocal laser scanning
      microscopy and the adherent force was measured by atomic force microscope (AFM). 
      The COX-2 mRNA level and PGE2 production were increased by Staphylococcus aureus.
      PGE2 promoted the growth and biofilm formation of Staphylococcus aureus, enhanced
      the attachment of Staphylococcus aureus to the human fibronectin as well as to
      the HOK cells. The transcription of fnbpB was up-regulated by PGE2 in both early 
      and middle exponential phase but not fnbpA. These results suggest that the
      activation of COX-2/PGE2 pathway in oral epithelial cell by Staphylococcus aureus
      can in turn facilitate the growth and the ability to adhere of the pathogen.
      These findings uncover a new function of PGE2 and may lead to the potential of
      COX-2/PGE2 targeting in the therapy of inflammation and cancer in both which the 
      COX-2/PGE2 pathway were observed activated.
FAU - Wang, Yuxia
AU  - Wang Y
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
FAU - Ren, Biao
AU  - Ren B
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
FAU - Zhou, Xuedong
AU  - Zhou X
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
FAU - Liu, Shiyu
AU  - Liu S
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
FAU - Zhou, Yujie
AU  - Zhou Y
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
FAU - Li, Bolei
AU  - Li B
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
FAU - Jiang, Yaling
AU  - Jiang Y
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
FAU - Li, Mingyun
AU  - Li M
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
FAU - Feng, Mingye
AU  - Feng M
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
FAU - Cheng, Lei
AU  - Cheng L
AUID- ORCID: http://orcid.org/0000-0002-2762-4740
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
AD  - Department of Operative Dentistry and Endodontics, West China Hospital of
      Stomatology, Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20170504
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Fibronectins)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Bacterial Adhesion/*physiology
MH  - Biofilms
MH  - Cells, Cultured
MH  - Cyclooxygenase 2/genetics/*metabolism
MH  - Dinoprostone/biosynthesis/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibronectins/metabolism
MH  - Humans
MH  - Microscopy, Atomic Force
MH  - Mouth Mucosa/enzymology/*metabolism/microbiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Staphylococcus aureus/*growth & development/physiology
PMC - PMC5417706
EDAT- 2017/05/05 06:00
MHDA- 2017/09/16 06:00
CRDT- 2017/05/05 06:00
PHST- 2016/12/09 00:00 [received]
PHST- 2017/04/24 00:00 [accepted]
PHST- 2017/05/05 06:00 [entrez]
PHST- 2017/05/05 06:00 [pubmed]
PHST- 2017/09/16 06:00 [medline]
AID - 10.1371/journal.pone.0177166 [doi]
AID - PONE-D-16-48771 [pii]
PST - epublish
SO  - PLoS One. 2017 May 4;12(5):e0177166. doi: 10.1371/journal.pone.0177166.
      eCollection 2017.