PMID- 28407288
OWN - NLM
STAT- MEDLINE
DCOM- 20171004
LR  - 20180122
IS  - 1527-3350 (Electronic)
IS  - 0270-9139 (Linking)
VI  - 66
IP  - 4
DP  - 2017 Oct
TI  - Efficient replication of blood-borne hepatitis C virus in human fetal liver stem 
      cells.
PG  - 1045-1057
LID - 10.1002/hep.29211 [doi]
AB  - The development of pathogenic mechanisms, specific antiviral treatments and
      preventive vaccines for hepatitis C virus (HCV) infection has been limited due to
      lack of cell culture models that can naturally imitate the entire HCV life cycle.
      Here, we established an HCV cell culture model based on human fetal liver stem
      cells (hFLSCs) that supports the entire blood-borne hepatitis C virus (bbHCV)
      life cycle. More than 90% of cells remained infected by various genotypes. bbHCV 
      was efficiently propagated, and progeny virus were infectious to hFLSCs. The
      virus could be passed efficiently between cells. The viral infectivity was
      partially blocked by specific antibodies or small interfering RNA against HCV
      entry factors, whereas HCV replication was inhibited by antiviral drugs. We
      observed viral particles of approximately 55 nm in diameter in both cell culture 
      media and infected cells after bbHCV infection. CONCLUSION: Our data show that
      the entire bbHCV life cycle could be naturally imitated in hFLSCs. This model is 
      expected to provide a powerful tool for exploring the process and the mechanism
      of bbHCV infection at the cellular level and for evaluating the treatment and
      preventive strategies of bbHCV infection. (Hepatology 2017;66:1045-1057).
CI  - (c) 2017 by the American Association for the Study of Liver Diseases.
FAU - Guo, Xuan
AU  - Guo X
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Wang, Shu
AU  - Wang S
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Qiu, Zhi-Gang
AU  - Qiu ZG
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Dou, Ya-Ling
AU  - Dou YL
AD  - Peking Union Medical College Hospital, Chinese Medical Academy, Beijing, China.
FAU - Liu, Wei-Li
AU  - Liu WL
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Yang, Dong
AU  - Yang D
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Shen, Zhi-Qiang
AU  - Shen ZQ
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Chen, Zhao-Li
AU  - Chen ZL
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Wang, Jing-Feng
AU  - Wang JF
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Zhang, Bin
AU  - Zhang B
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Wang, Xin-Wei
AU  - Wang XW
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Guo, Xiang-Fei
AU  - Guo XF
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Zhang, Xue-Lian
AU  - Zhang XL
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Jin, Min
AU  - Jin M
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
FAU - Li, Jun-Wen
AU  - Li JW
AD  - Department of Environment and Health, Tianjin Institute of Health and
      Environmental Medicine, Key Laboratory of Risk Assessment and Control for
      Environment & Food Safety, Tianjin, China.
LA  - eng
PT  - Evaluation Studies
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170826
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (Viral Proteins)
SB  - IM
MH  - *Fetal Stem Cells
MH  - Hepacivirus/*physiology
MH  - Humans
MH  - Liver/*cytology/virology
MH  - *Models, Biological
MH  - Primary Cell Culture
MH  - Viral Proteins/biosynthesis
MH  - Virus Release
MH  - *Virus Replication
EDAT- 2017/04/14 06:00
MHDA- 2017/10/05 06:00
CRDT- 2017/04/14 06:00
PHST- 2017/02/02 00:00 [received]
PHST- 2017/04/05 00:00 [accepted]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2017/10/05 06:00 [medline]
PHST- 2017/04/14 06:00 [entrez]
AID - 10.1002/hep.29211 [doi]
PST - ppublish
SO  - Hepatology. 2017 Oct;66(4):1045-1057. doi: 10.1002/hep.29211. Epub 2017 Aug 26.