PMID- 28380055
OWN - NLM
STAT- MEDLINE
DCOM- 20170830
LR  - 20170830
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 4
DP  - 2017
TI  - Field evaluation of a blood based test for active tuberculosis in endemic
      settings.
PG  - e0173359
LID - 10.1371/journal.pone.0173359 [doi]
AB  - Over 9 million new active tuberculosis (TB) cases emerge each year from an
      enormous pool of 2 billion individuals latently infected with Mycobacterium
      tuberculosis (M. tb.) worldwide. About 3 million new TB cases per year are
      unaccounted for, and 1.5 million die. TB, however, is generally curable if
      diagnosed correctly and in a timely manner. The current diagnostic methods for
      TB, including state-of-the-art molecular tests, have failed in delivering the
      capacity needed in endemic countries to curtail this ongoing pandemic. Efficient,
      cost effective and scalable diagnostic approaches are critically needed. We
      report a multiplex TB serology panel using microbead suspension array containing 
      a combination of 11 M.tb. antigens that demonstrated overall sensitivity of 91%
      in serum/plasma samples from TB patients confirmed by culture. Group wise
      sensitivities for sputum smear positive and negative patients were 95%, and 88%, 
      respectively. Specificity of the test was 96% in untreated COPD patients and 91% 
      in general healthy population. The sensitivity of this test is superior to that
      of the frontline sputum smear test with a comparable specificity (30-70%, and
      93-99%, respectively). The multiplex serology test can be performed with
      scalability from 1 to 360 patients per day, and is amenable to automation for
      higher (1000s per day) throughput, thus enabling a scalable clinical work flow
      model for TB endemic countries. Taken together, the above results suggest that
      well defined antibody profiles in blood, analyzed by an appropriate technology
      platform, offer a valuable approach to TB diagnostics in endemic countries.
FAU - Khaliq, Aasia
AU  - Khaliq A
AD  - College of Earth and Environmental Sciences, University of the Punjab, Lahore,
      Pakistan.
FAU - Ravindran, Resmi
AU  - Ravindran R
AD  - Department of Pathology and Laboratory Medicine, University of California, Davis,
      California, United States of America.
FAU - Hussainy, Syed Fahadulla
AU  - Hussainy SF
AD  - Department of Computer Science, School of Science and Engineering, Lahore
      University of Management Sciences, Lahore, Pakistan.
FAU - Krishnan, Viwanathan V
AU  - Krishnan VV
AD  - Department of Pathology and Laboratory Medicine, University of California, Davis,
      California, United States of America.
AD  - Department of Chemistry, California State University, Fresno, California, United 
      States of America.
FAU - Ambreen, Atiqa
AU  - Ambreen A
AD  - Gulab Devi Chest Hospital, Lahore, Pakistan.
FAU - Yusuf, Noshin Wasim
AU  - Yusuf NW
AD  - Department of Pathology, Allama Iqbal Medical College, Lahore, Pakistan.
FAU - Irum, Shagufta
AU  - Irum S
AD  - Department of Pathology, Allama Iqbal Medical College, Lahore, Pakistan.
FAU - Rashid, Abdul
AU  - Rashid A
AD  - Gulab Devi Chest Hospital, Lahore, Pakistan.
FAU - Jamil, Muhammad
AU  - Jamil M
AD  - Gulab Devi Chest Hospital, Lahore, Pakistan.
FAU - Zaffar, Fareed
AU  - Zaffar F
AD  - Department of Computer Science, School of Science and Engineering, Lahore
      University of Management Sciences, Lahore, Pakistan.
FAU - Chaudhry, Muhammad Nawaz
AU  - Chaudhry MN
AD  - College of Earth and Environmental Sciences, University of the Punjab, Lahore,
      Pakistan.
FAU - Gupta, Puneet K
AU  - Gupta PK
AD  - NextGen In Vitro Diagnostics, New Delhi, India.
FAU - Akhtar, Muhammad Waheed
AU  - Akhtar MW
AD  - School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
FAU - Khan, Imran H
AU  - Khan IH
AD  - Department of Pathology and Laboratory Medicine, University of California, Davis,
      California, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20170405
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adult
MH  - Female
MH  - Hematologic Tests/*methods
MH  - Humans
MH  - Male
MH  - Mycobacterium tuberculosis/immunology
MH  - Plasma/microbiology
MH  - Sensitivity and Specificity
MH  - Serologic Tests/methods
MH  - Sputum/microbiology
MH  - Tuberculosis, Pulmonary/*blood/*diagnosis/microbiology
MH  - Young Adult
PMC - PMC5381859
EDAT- 2017/04/06 06:00
MHDA- 2017/08/31 06:00
CRDT- 2017/04/06 06:00
PHST- 2016/11/21 00:00 [received]
PHST- 2017/02/19 00:00 [accepted]
PHST- 2017/04/06 06:00 [entrez]
PHST- 2017/04/06 06:00 [pubmed]
PHST- 2017/08/31 06:00 [medline]
AID - 10.1371/journal.pone.0173359 [doi]
AID - PONE-D-16-46248 [pii]
PST - epublish
SO  - PLoS One. 2017 Apr 5;12(4):e0173359. doi: 10.1371/journal.pone.0173359.
      eCollection 2017.