PMID- 28323835
OWN - NLM
STAT- MEDLINE
DCOM- 20170823
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 3
DP  - 2017
TI  - Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital,
      Thailand.
PG  - e0173744
LID - 10.1371/journal.pone.0173744 [doi]
AB  - Despite the strong association between Cryptococcus neoformans infection and the 
      Human immunodeficiency virus (HIV) status of patients globally, most
      cryptococcosis cases in Far East Asia occur in non-HIV individuals. Molecular
      epidemiological studies, using multilocus sequence typing (MLST), have shown that
      more than 95% of cryptococcal strains belong to a specific subtype of VNI.
      However, this association has never been specifically examined in other parts of 
      Asia. Therefore, in this study, we investigated the VNIc/ST5 genotype
      distribution among cryptococcosis patients in Thailand. Fifty-one C. neoformans
      isolates were collected from clinical samples in Siriraj Hospital, Bangkok,
      Thailand. The strains were predominantly isolated from HIV-positive patients
      (88.57%) and all were molecular type VNI MATalpha. An MLST analysis identified
      five sequence types (ST) in Siriraj Hospital, of which ST4 (45.10%) and ST6
      (35.29%) were most common, and ST5 (15.69%), ST32 (1.96%), and ST93 (1.96) were
      less common. Contrary to reports from Far East Asia, ST5 was predominantly
      (83.33%) found in HIV patients (P = 0.657), and there was no significant change
      in the prevalence of ST5 over the past 10 years (P = 0.548). A further analysis
      of comorbidities showed higher morbidity and delays in the cryptococcal diagnosis
      in patients with tuberculosis coinfection or without HIV. Our study suggests that
      although the Thai population is genetically closely related to the Far East Asian
      population, ST5 is not associated with non-HIV status in Thailand. Therefore,
      this association may not be related to the host's genetic background. However,
      its mechanism remains unclear.
FAU - Hatthakaroon, Chanin
AU  - Hatthakaroon C
AD  - Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol
      University, Bangkok Noi, Bangkok, Thailand.
FAU - Pharkjaksu, Sujiraphong
AU  - Pharkjaksu S
AD  - Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol
      University, Bangkok Noi, Bangkok, Thailand.
FAU - Chongtrakool, Piriyaporn
AU  - Chongtrakool P
AD  - Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol
      University, Bangkok Noi, Bangkok, Thailand.
FAU - Suwannakarn, Kamol
AU  - Suwannakarn K
AD  - Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol
      University, Bangkok Noi, Bangkok, Thailand.
FAU - Kiratisin, Pattarachai
AU  - Kiratisin P
AD  - Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol
      University, Bangkok Noi, Bangkok, Thailand.
FAU - Ngamskulrungroj, Popchai
AU  - Ngamskulrungroj P
AUID- ORCID: http://orcid.org/0000-0001-5162-5498
AD  - Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol
      University, Bangkok Noi, Bangkok, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20170321
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Comorbidity
MH  - Cryptococcosis/complications/*epidemiology/*genetics
MH  - Cryptococcus neoformans/*genetics/isolation & purification
MH  - Female
MH  - Genotype
MH  - HIV Infections/complications/epidemiology
MH  - Hospitals, University
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Middle Aged
MH  - Molecular Epidemiology
MH  - Mycological Typing Techniques
MH  - Phylogeny
MH  - Thailand/epidemiology
MH  - Tuberculosis/complications/epidemiology
MH  - Young Adult
PMC - PMC5360237
EDAT- 2017/03/23 06:00
MHDA- 2017/08/24 06:00
CRDT- 2017/03/22 06:00
PHST- 2016/12/02 00:00 [received]
PHST- 2017/02/24 00:00 [accepted]
PHST- 2017/03/22 06:00 [entrez]
PHST- 2017/03/23 06:00 [pubmed]
PHST- 2017/08/24 06:00 [medline]
AID - 10.1371/journal.pone.0173744 [doi]
AID - PONE-D-16-45086 [pii]
PST - epublish
SO  - PLoS One. 2017 Mar 21;12(3):e0173744. doi: 10.1371/journal.pone.0173744.
      eCollection 2017.