PMID- 28222189
OWN - NLM
STAT- MEDLINE
DCOM- 20170817
LR  - 20190208
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 2
DP  - 2017
TI  - Molecular epidemiology of Mycobacterium tuberculosis complex in Brussels,
      2010-2013.
PG  - e0172554
LID - 10.1371/journal.pone.0172554 [doi]
AB  - The tuberculosis (TB) incidence rate in Brussels-Capital Region is 3-fold higher 
      than in Belgium as a whole. Eight years after the realization of initial
      prospective population-based molecular epidemiology investigations in this
      Region, a similar study over the period 2010-2013 was conducted. TB strains
      isolated from 945 patients were submitted to genotyping by standardized
      24-locus-MIRU-VNTR typing and spoligotyping. The phylogenetic analysis showed
      that the LAM (16.7%) and Haarlem (15.7%) branches are the two most prevalent TB
      lineages circulating in Brussels. Analysis of the MDR subgroup showed an
      association with Beijing strains (39.9%) and patients native of Eastern Europe
      (40.7%). Genotyping detected 113 clusters involving 321 patients, giving a recent
      transmission index of 22.9%. Molecular-guided epidemiological investigations and 
      routine surveillance activities revealed family transmission or social contact
      for patients distributed over 34 clusters. Most of the patients were foreign-born
      (75.7%). However, cluster analysis revealed only limited trans-national
      transmission. Comparison with the previous study shows a stable epidemiological
      situation except for the mean age difference between Belgian-born and
      foreign-born patients which has disappeared. This study confirms that molecular
      epidemiology has become an important determinant for TB control programs.
      However, sufficient financial means need to be available to perform all required 
      epidemiological investigations.
FAU - Vluggen, Christelle
AU  - Vluggen C
AD  - Bacterial Diseases Service, Operational Direction Communicable and Infectious
      Diseases, Scientific Institute of Public Health (WIV-ISP), Brussels, Belgium.
FAU - Soetaert, Karine
AU  - Soetaert K
AD  - Bacterial Diseases Service, Operational Direction Communicable and Infectious
      Diseases, Scientific Institute of Public Health (WIV-ISP), Brussels, Belgium.
FAU - Groenen, Guido
AU  - Groenen G
AD  - Belgian Lung and Tuberculosis Association, Brussels, Belgium.
FAU - Wanlin, Maryse
AU  - Wanlin M
AD  - Belgian Lung and Tuberculosis Association, Brussels, Belgium.
FAU - Spitaels, Martine
AU  - Spitaels M
AD  - Belgian Lung and Tuberculosis Association, Brussels, Belgium.
FAU - Arrazola de Onate, Wouter
AU  - Arrazola de Onate W
AD  - Belgian Lung and Tuberculosis Association, Brussels, Belgium.
FAU - Fauville-Dufaux, Maryse
AU  - Fauville-Dufaux M
AD  - Bacterial Diseases Service, Operational Direction Communicable and Infectious
      Diseases, Scientific Institute of Public Health (WIV-ISP), Brussels, Belgium.
FAU - Saegerman, Claude
AU  - Saegerman C
AD  - Research Unit of Epidemiology and Risk Analysis applied to Veterinary Sciences
      (UREAR-ULg), Fundamental and Applied Research for Animal and Health (FARAH)
      Centre, Liege, Belgium.
FAU - Mathys, Vanessa
AU  - Mathys V
AUID- ORCID: http://orcid.org/0000-0001-5218-927X
AD  - Bacterial Diseases Service, Operational Direction Communicable and Infectious
      Diseases, Scientific Institute of Public Health (WIV-ISP), Brussels, Belgium.
LA  - eng
PT  - Journal Article
DEP - 20170221
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bacterial Typing Techniques/methods
MH  - Belgium/epidemiology
MH  - Child
MH  - Cluster Analysis
MH  - Contact Tracing
MH  - Drug Resistance, Multiple, Bacterial
MH  - Emigrants and Immigrants
MH  - Family Health
MH  - Female
MH  - Genetic Variation
MH  - Genotype
MH  - Hospitals, Urban
MH  - Humans
MH  - Incidence
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/classification/drug effects/genetics/*isolation &
      purification
MH  - Phylogeny
MH  - Population Surveillance
MH  - Tuberculosis/economics/epidemiology/*microbiology/transmission
MH  - Tuberculosis, Multidrug-Resistant/epidemiology/microbiology/transmission
MH  - Urban Health
MH  - Young Adult
PMC - PMC5319770
EDAT- 2017/02/22 06:00
MHDA- 2017/08/18 06:00
CRDT- 2017/02/22 06:00
PHST- 2016/12/08 00:00 [received]
PHST- 2017/02/06 00:00 [accepted]
PHST- 2017/02/22 06:00 [entrez]
PHST- 2017/02/22 06:00 [pubmed]
PHST- 2017/08/18 06:00 [medline]
AID - 10.1371/journal.pone.0172554 [doi]
AID - PONE-D-16-48557 [pii]
PST - epublish
SO  - PLoS One. 2017 Feb 21;12(2):e0172554. doi: 10.1371/journal.pone.0172554.
      eCollection 2017.