PMID- 28202799
OWN - NLM
STAT- MEDLINE
DCOM- 20171031
LR  - 20181113
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 55
IP  - 5
DP  - 2017 May
TI  - Clonal Complexity in Mycobacterium tuberculosis Can Hamper Diagnostic Procedures.
PG  - 1388-1395
LID - 10.1128/JCM.00149-17 [doi]
AB  - Clonal complexity is increasingly accepted in Mycobacterium tuberculosis
      infection, including mixed infections by >/=2 strains, which usually occur in
      settings with a high burden of tuberculosis and/or a high risk of overexposure to
      infected patients. Mixed infections can hamper diagnostic procedures; obtaining
      an accurate antibiogram is difficult when the susceptibility patterns of the
      strains differ. Here, we show how mixed infections can also prove challenging for
      other diagnostic procedures, even outside settings where mixed infections are
      traditionally expected. We show how an unnoticed mixed infection in an
      HIV-positive patient diagnosed in Madrid, Spain, with differences in the
      representativeness of the coinfecting strains in different sputum samples,
      markedly complicated the resolution of a laboratory cross-contamination false
      positivity alert.
CI  - Copyright (c) 2017 American Society for Microbiology.
FAU - Perez-Lago, Laura
AU  - Perez-Lago L
AD  - Servicio Microbiologia Clinica y Enfermedades Infecciosas, Hospital General
      Universitario Gregorio Maranon, Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - CIBER Enfermedades Respiratorias, CIBERES, Spain.
FAU - Herranz, Marta
AU  - Herranz M
AD  - Servicio Microbiologia Clinica y Enfermedades Infecciosas, Hospital General
      Universitario Gregorio Maranon, Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - CIBER Enfermedades Respiratorias, CIBERES, Spain.
FAU - Navarro, Yurena
AU  - Navarro Y
AD  - Servicio Microbiologia Clinica y Enfermedades Infecciosas, Hospital General
      Universitario Gregorio Maranon, Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - CIBER Enfermedades Respiratorias, CIBERES, Spain.
FAU - Ruiz Serrano, Maria Jesus
AU  - Ruiz Serrano MJ
AD  - Servicio Microbiologia Clinica y Enfermedades Infecciosas, Hospital General
      Universitario Gregorio Maranon, Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - CIBER Enfermedades Respiratorias, CIBERES, Spain.
FAU - Miralles, Pilar
AU  - Miralles P
AD  - Servicio Microbiologia Clinica y Enfermedades Infecciosas, Hospital General
      Universitario Gregorio Maranon, Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
FAU - Bouza, Emilio
AU  - Bouza E
AD  - Servicio Microbiologia Clinica y Enfermedades Infecciosas, Hospital General
      Universitario Gregorio Maranon, Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - CIBER Enfermedades Respiratorias, CIBERES, Spain.
AD  - Departamento de Medicina, Facultad de Medicina, Universidad Complutense de
      Madrid, Madrid, Spain.
FAU - Garcia-de-Viedma, Dario
AU  - Garcia-de-Viedma D
AD  - Servicio Microbiologia Clinica y Enfermedades Infecciosas, Hospital General
      Universitario Gregorio Maranon, Madrid, Spain dgviedma2@gmail.com.
AD  - Instituto de Investigacion Sanitaria Gregorio Maranon, Madrid, Spain.
AD  - CIBER Enfermedades Respiratorias, CIBERES, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170215
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - Coinfection/microbiology/virology
MH  - False Positive Reactions
MH  - HIV Infections/*diagnosis/virology
MH  - Humans
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/*classification/genetics
MH  - Spain
MH  - Sputum/microbiology
MH  - Tuberculosis, Pulmonary/*diagnosis/microbiology
PMC - PMC5405256
OTO - NOTNLM
OT  - *clonal complexity
OT  - *diagnostic procedures
OT  - *mixed infection
OT  - *tuberculosis
EDAT- 2017/02/17 06:00
MHDA- 2017/11/01 06:00
CRDT- 2017/02/17 06:00
PHST- 2017/01/25 00:00 [received]
PHST- 2017/02/07 00:00 [accepted]
PHST- 2017/02/17 06:00 [pubmed]
PHST- 2017/11/01 06:00 [medline]
PHST- 2017/02/17 06:00 [entrez]
AID - JCM.00149-17 [pii]
AID - 10.1128/JCM.00149-17 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2017 May;55(5):1388-1395. doi: 10.1128/JCM.00149-17. Epub 2017 
      Feb 15.