PMID- 28089831
OWN - NLM
STAT- MEDLINE
DCOM- 20170829
LR  - 20180414
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 390
DP  - 2017 Apr 1
TI  - Monomethyltransferase SETD8 regulates breast cancer metabolism via stabilizing
      hypoxia-inducible factor 1alpha.
PG  - 1-10
LID - S0304-3835(17)30020-4 [pii]
LID - 10.1016/j.canlet.2016.12.038 [doi]
AB  - SETD8 is a methyltransferase that specifically catalyzes the monomethylation of
      lysine 20 on histone H4. Previous studies have demonstrated that SETD8 is
      associated with proper cell cycle progression, DNA damage response, and
      transcriptional regulation. A recent study revealed that SETD8 played an
      important role in epithelial-mesenchymal transition (EMT) in association with
      TWIST and enhanced metastatic potential of breast cancer cells. However, the
      contribution of SETD8 to metabolism reprogramming, one hallmark of cancer, has
      never been reported. In this study, we report that SETD8 was a positive regulator
      of anabolic metabolism. SETD8 reprograms breast cancer cell metabolism through
      hypoxia-inducible factor 1alpha (HIF1alpha) mediated process. Mechanistic studies
      indicated that SETD8 stabilized HIF1alpha protein level through
      post-transcriptional regulation. Moreover, we demonstrated that SETD8 was a
      HIF1alpha transcription target. In clinical breast cancer patient tissues, we
      observed a positive correlation of SETD8 with HIF1alpha and HIF1alpha target
      genes. Taken together, we validated SETD8 as a novel metabolic reprogramming
      regulator, and our mechanistic studies shed light on a novel function of SETD8 in
      breast cancer malignant properties maintenance.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Huang, Run
AU  - Huang R
AD  - Department of Breast Surgery, Shanghai Jiao Tong University Affiliated Shanghai
      Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
FAU - Yu, Yang
AU  - Yu Y
AD  - Department of Breast Surgery, Zhejiang Cancer Hospital, 38 Guangji Road, Hangzhou
      310022, China.
FAU - Zong, Xiangyun
AU  - Zong X
AD  - Department of Breast Surgery, Shanghai Jiao Tong University Affiliated Shanghai
      Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. Electronic
      address: tigerzong@msn.com.
FAU - Li, Xiaolin
AU  - Li X
AD  - Department of Breast Surgery, Shanghai Jiao Tong University Affiliated Shanghai
      Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
FAU - Ma, Lisi
AU  - Ma L
AD  - Department of Breast Surgery, Shanghai Jiao Tong University Affiliated Shanghai
      Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
FAU - Zheng, Qi
AU  - Zheng Q
AD  - Department of General Surgery, Shanghai Jiao Tong University Affiliated Shanghai 
      Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170113
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (SETD8 protein, human)
SB  - IM
MH  - Biomarkers, Tumor/metabolism
MH  - Blotting, Western
MH  - Breast Neoplasms/*enzymology/physiopathology
MH  - Cell Line, Tumor
MH  - Female
MH  - Histone-Lysine N-Methyltransferase/*metabolism
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/chemistry/*metabolism
MH  - Immunohistochemistry
MH  - Mass Spectrometry
MH  - Protein Stability
OTO - NOTNLM
OT  - *Breast cancer
OT  - *Cancer metabolism
OT  - *Glycolysis
OT  - *HIF1alpha
OT  - *SETD8
EDAT- 2017/01/17 06:00
MHDA- 2017/08/30 06:00
CRDT- 2017/01/17 06:00
PHST- 2016/09/19 00:00 [received]
PHST- 2016/12/03 00:00 [revised]
PHST- 2016/12/23 00:00 [accepted]
PHST- 2017/01/17 06:00 [pubmed]
PHST- 2017/08/30 06:00 [medline]
PHST- 2017/01/17 06:00 [entrez]
AID - S0304-3835(17)30020-4 [pii]
AID - 10.1016/j.canlet.2016.12.038 [doi]
PST - ppublish
SO  - Cancer Lett. 2017 Apr 1;390:1-10. doi: 10.1016/j.canlet.2016.12.038. Epub 2017
      Jan 13.