PMID- 28045981
OWN - NLM
STAT- MEDLINE
DCOM- 20170905
LR  - 20190109
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 1
DP  - 2017
TI  - HPV Genotyping of Modified General Primer-Amplicons Is More Analytically
      Sensitive and Specific by Sequencing than by Hybridization.
PG  - e0169074
LID - 10.1371/journal.pone.0169074 [doi]
AB  - Sensitive and specific genotyping of human papillomaviruses (HPVs) is important
      for population-based surveillance of carcinogenic HPV types and for monitoring
      vaccine effectiveness. Here we compare HPV genotyping by Next Generation
      Sequencing (NGS) to an established DNA hybridization method. In DNA isolated from
      urine, the overall analytical sensitivity of NGS was found to be 22% higher than 
      that of hybridization. NGS was also found to be the most specific method and
      expanded the detection repertoire beyond the 37 types of the DNA hybridization
      assay. Furthermore, NGS provided an increased resolution by identifying genetic
      variants of individual HPV types. The same Modified General Primers
      (MGP)-amplicon was used in both methods. The NGS method is described in detail to
      facilitate implementation in the clinical microbiology laboratory and includes
      suggestions for new standards for detection and calling of types and variants
      with improved resolution.
FAU - Meisal, Roger
AU  - Meisal R
AD  - Department of Microbiology and Infection Control, Akershus University Hospital,
      Lorenskog, Norway.
FAU - Rounge, Trine Ballestad
AU  - Rounge TB
AD  - Department of Research, Cancer Registry of Norway, Oslo, Norway.
FAU - Christiansen, Irene Kraus
AU  - Christiansen IK
AD  - Department of Microbiology and Infection Control, Akershus University Hospital,
      Lorenskog, Norway.
FAU - Eieland, Alexander Kirkeby
AU  - Eieland AK
AD  - Department of Microbiology and Infection Control, Akershus University Hospital,
      Lorenskog, Norway.
FAU - Worren, Merete Molton
AU  - Worren MM
AD  - Bioinformatics Core Facility, Institute for Cancer Genetics and Informatics, The 
      Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway.
FAU - Molden, Tor Faksvaag
AU  - Molden TF
AD  - Department of Vaccines, Norwegian Institute of Public Health, Oslo, Norway.
FAU - Kommedal, Oyvind
AU  - Kommedal O
AD  - Department of Microbiology, Haukeland University Hospital, Bergen, Norway.
FAU - Hovig, Eivind
AU  - Hovig E
AD  - Department of Informatics, University of Oslo, Oslo, Norway.
AD  - Department of Tumor Biology, Institute of Cancer Research and Institute for
      Cancer Genetics and Informatics, The Norwegian Radium Hospital, Oslo University
      Hospital, Oslo, Norway.
FAU - Leegaard, Truls Michael
AU  - Leegaard TM
AD  - Department of Microbiology and Infection Control, Akershus University Hospital,
      Lorenskog, Norway.
AD  - Division of Medicine and Laboratory Sciences, University of Oslo, Oslo Norway.
FAU - Ambur, Ole Herman
AU  - Ambur OH
AUID- ORCID: http://orcid.org/0000-0001-9870-6375
AD  - Department of Microbiology and Infection Control, Akershus University Hospital,
      Lorenskog, Norway.
AD  - Department of Life Sciences and Health, Oslo and Akershus University College of
      Applied Sciences, Oslo, Norway.
LA  - eng
PT  - Journal Article
DEP - 20170103
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Chlamydia Infections
MH  - DNA Primers/*genetics
MH  - DNA, Viral/genetics
MH  - Female
MH  - Genetic Variation
MH  - Genotyping Techniques/methods
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Humans
MH  - Hybridization, Genetic
MH  - *Nucleic Acid Hybridization
MH  - Papillomaviridae/*genetics
MH  - Papillomavirus Infections/diagnosis/*virology
MH  - Sensitivity and Specificity
MH  - Uterine Cervical Neoplasms/diagnosis/*virology
MH  - Young Adult
PMC - PMC5207713
COIS- The authors have declared that no competing interests exist.
EDAT- 2017/01/04 06:00
MHDA- 2017/09/07 06:00
CRDT- 2017/01/04 06:00
PHST- 2016/04/18 00:00 [received]
PHST- 2016/12/12 00:00 [accepted]
PHST- 2017/01/04 06:00 [entrez]
PHST- 2017/01/04 06:00 [pubmed]
PHST- 2017/09/07 06:00 [medline]
AID - 10.1371/journal.pone.0169074 [doi]
AID - PONE-D-16-15658 [pii]
PST - epublish
SO  - PLoS One. 2017 Jan 3;12(1):e0169074. doi: 10.1371/journal.pone.0169074.
      eCollection 2017.