PMID- 27879516
DCOM- 20170719
LR  - 20170719
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 41
IP  - 2
DP  - 2017 Feb
TI  - Performance of a Commercially Available MAL Antibody in the Diagnosis of Primary 
      Mediastinal Large B-Cell Lymphoma.
PG  - 189-194
LID - 10.1097/PAS.0000000000000771 [doi]
AB  - Myelin and lymphocyte (MAL) protein has been previously reported as a highly
      specific marker for distinguishing primary mediastinal large B-cell lymphoma
      (PMBL) from diffuse large B-cell lymphoma, not otherwise specified (DLBCL, NOS). 
      However, there has not been a commercially available MAL antibody for
      immunohistochemistry. We identified a commercially available MAL monoclonal
      antibody and evaluated it by immunohistochemistry on 43 cases of PMBL and 63
      cases of DLBCL, NOS. We also compared this with a CD200 antibody that was
      previously reported useful in distinguishing PMBL and DLBCL, NOS. A threshold of 
      10% positive tumor cells was used to determine positive protein expression. MAL
      was expressed in 72% cases of PMBL and 0% of cases of DLBCL, NOS
      (sensitivity=72%, specificity=100%). CD200 was expressed in 81% of PMBL cases and
      13% of DLBCL, NOS cases (sensitivity=81%, specificity=87%). To our knowledge,
      this is the first report on the utility of a commercially available MAL
      monoclonal antibody in the diagnosis of PMBL. There is a high specificity with
      good sensitivity in distinguishing PMBL from DLBCL, NOS, similar to previous
      studies with a noncommercial source. This antibody will likely prove useful in
      identifying cases of PMBL in routine practice.
FAU - Gentry, Michael
AU  - Gentry M
AD  - Department of Laboratory Medicine, Robert J. Tomsich Institute of Pathology and
      Laboratory Medicine, Cleveland Clinic, Cleveland, OH.
FAU - Bodo, Juraj
AU  - Bodo J
FAU - Durkin, Lisa
AU  - Durkin L
FAU - Hsi, Eric D
AU  - Hsi ED
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Myelin and Lymphocyte-Associated Proteolipid Proteins)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Biomarkers, Tumor/*analysis
MH  - Blotting, Western
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphoma, B-Cell/*diagnosis
MH  - Lymphoma, Large B-Cell, Diffuse/diagnosis
MH  - Male
MH  - Mediastinal Neoplasms/*diagnosis
MH  - Middle Aged
MH  - Myelin and Lymphocyte-Associated Proteolipid Proteins/*analysis
MH  - Sensitivity and Specificity
MH  - Young Adult
EDAT- 2016/11/24 06:00
MHDA- 2017/07/20 06:00
CRDT- 2016/11/24 06:00
PHST- 2016/11/24 06:00 [pubmed]
PHST- 2017/07/20 06:00 [medline]
PHST- 2016/11/24 06:00 [entrez]
AID - 10.1097/PAS.0000000000000771 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2017 Feb;41(2):189-194. doi: 10.1097/PAS.0000000000000771.