PMID- 27707590
OWN - NLM
STAT- MEDLINE
DCOM- 20170424
LR  - 20181113
IS  - 1097-6787 (Electronic)
IS  - 0190-9622 (Linking)
VI  - 76
IP  - 1
DP  - 2017 Jan
TI  - Development and validation of a noninvasive 2-gene molecular assay for cutaneous 
      melanoma.
PG  - 114-120.e2
LID - S0190-9622(16)30589-8 [pii]
LID - 10.1016/j.jaad.2016.07.038 [doi]
AB  - BACKGROUND: Clinical and histopathologic assessment of pigmented skin lesions
      remains challenging even for experts. Differentiated and accurate noninvasive
      diagnostic modalities are highly desirable. OBJECTIVE: We sought to provide
      clinicians with such a tool. METHODS: A 2-gene classification method based on
      LINC00518 and preferentially expressed antigen in melanoma (PRAME) gene
      expression was evaluated and validated in 555 pigmented lesions (157 training and
      398 validation samples) obtained noninvasively via adhesive patch biopsy. Results
      were compared with standard histopathologic assessment in lesions with a
      consensus diagnosis among 3 experienced dermatopathologists. RESULTS: In 398
      validation samples (87 melanomas and 311 nonmelanomas), LINC00518 and/or PRAME
      detection appropriately differentiated melanoma from nonmelanoma samples with a
      sensitivity of 91% and a specificity of 69%. We established LINC00518 and PRAME
      in both adhesive patch melanoma samples and underlying formalin fixed paraffin
      embedded (FFPE) samples of surgically excised primary melanomas and in melanoma
      lymph node metastases. LIMITATIONS: This technology cannot be used on mucous
      membranes, palms of hands, and soles of feet. CONCLUSIONS: This noninvasive
      2-gene pigmented lesion assay classifies pigmented lesions into melanoma and
      nonmelanoma groups and may serve as a tool to help with diagnostic challenges
      that may be inherently linked to the visual image and pattern recognition
      approach.
CI  - Copyright (c) 2016 American Academy of Dermatology, Inc. Published by Elsevier
      Inc. All rights reserved.
FAU - Gerami, Pedram
AU  - Gerami P
AD  - Department of Dermatology, Northwestern University, Chicago, Illinois. Electronic
      address: pgerami1@nm.org.
FAU - Yao, Zuxu
AU  - Yao Z
AD  - DermTech Inc, La Jolla, California.
FAU - Polsky, David
AU  - Polsky D
AD  - Ronald O. Perelman Department of Dermatology, New York University School of
      Medicine, New York University Langone Medical Center, New York, New York; Ronald 
      O. Perelman Department of Pathology, New York University School of Medicine, New 
      York University Langone Medical Center, New York, New York.
FAU - Jansen, Burkhard
AU  - Jansen B
AD  - DermTech Inc, La Jolla, California.
FAU - Busam, Klaus
AU  - Busam K
AD  - Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New
      York.
FAU - Ho, Jonhan
AU  - Ho J
AD  - Department of Dermatology, University of Pittsburgh, Pittsburgh, Pennsylvania.
FAU - Martini, Mary
AU  - Martini M
AD  - Department of Dermatology, Northwestern University, Chicago, Illinois.
FAU - Ferris, Laura K
AU  - Ferris LK
AD  - Department of Dermatology, University of Pittsburgh, Pittsburgh, Pennsylvania.
LA  - eng
GR  - P30 CA008748/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Validation Studies
DEP - 20161001
PL  - United States
TA  - J Am Acad Dermatol
JT  - Journal of the American Academy of Dermatology
JID - 7907132
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (PRAME protein, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (long non-coding RNA LINC00518, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, Neoplasm/*genetics
MH  - Biopsy/methods
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Male
MH  - Melanoma/diagnosis/*genetics/secondary
MH  - Middle Aged
MH  - Nevus, Pigmented/diagnosis/*genetics/pathology
MH  - RNA, Long Noncoding/*genetics
MH  - RNA, Neoplasm/*analysis
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/diagnosis/*genetics/pathology
MH  - Young Adult
PMC - PMC5599145
MID - NIHMS902660
OTO - NOTNLM
OT  - *LINC00518
OT  - *biopsy
OT  - *gene expression
OT  - *histopathology
OT  - *melanoma
OT  - *noninvasive
OT  - *preferentially expressed antigen in melanoma
EDAT- 2016/10/07 06:00
MHDA- 2017/04/25 06:00
CRDT- 2016/10/07 06:00
PHST- 2016/05/09 00:00 [received]
PHST- 2016/07/14 00:00 [revised]
PHST- 2016/07/19 00:00 [accepted]
PHST- 2016/10/07 06:00 [pubmed]
PHST- 2017/04/25 06:00 [medline]
PHST- 2016/10/07 06:00 [entrez]
AID - S0190-9622(16)30589-8 [pii]
AID - 10.1016/j.jaad.2016.07.038 [doi]
PST - ppublish
SO  - J Am Acad Dermatol. 2017 Jan;76(1):114-120.e2. doi: 10.1016/j.jaad.2016.07.038.
      Epub 2016 Oct 1.