PMID- 27585214
DCOM- 20161213
LR  - 20161230
IS  - 1531-7021 (Electronic)
IS  - 1040-8738 (Linking)
VI  - 27
IP  - 6
DP  - 2016 Nov
TI  - Current and future molecular diagnostics for ocular infectious diseases.
PG  - 561-567
AB  - PURPOSE OF REVIEW: Confirmation of ocular infections can pose great challenges to
      the clinician. A fundamental limitation is the small amounts of specimen that can
      be obtained from the eye. Molecular diagnostics can circumvent this limitation
      and have been shown to be more sensitive than conventional culture. The purpose
      of this review is to describe new molecular methods and to discuss the
      applications of next-generation sequencing-based approaches in the diagnosis of
      ocular infections. RECENT FINDINGS: Efforts have focused on improving the
      sensitivity of pathogen detection using molecular methods. This review describes 
      a new molecular target for Toxoplasma gondii-directed polymerase chain reaction
      assays. Molecular diagnostics for Chlamydia trachomatis and Acanthamoeba species 
      are also discussed. Finally, we describe a hypothesis-free approach, metagenomic 
      deep sequencing, which can detect DNA and RNA pathogens from a single specimen in
      one test. In some cases, this method can provide the geographic location and
      timing of the infection. SUMMARY: Pathogen-directed PCRs have been powerful tools
      in the diagnosis of ocular infections for over 20 years. The use of
      next-generation sequencing-based approaches, when available, will further improve
      sensitivity of detection with the potential to improve patient care.
FAU - Doan, Thuy
AU  - Doan T
AD  - aF.I. Proctor Foundation bDepartment of Ophthalmology, University of California, 
      San Francisco cDepartment of Pathology dDivision of Infectious Diseases and
      Geographic Medicine, Department of Medicine, Stanford University School of
      Medicine, Stanford, California, USA.
FAU - Pinsky, Benjamin A
AU  - Pinsky BA
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Curr Opin Ophthalmol
JT  - Current opinion in ophthalmology
JID - 9011108
SB  - IM
MH  - Acanthamoeba/genetics
MH  - Amebiasis/complications/diagnosis
MH  - Chlamydia Infections/complications/*diagnosis
MH  - Chlamydia trachomatis/*genetics
MH  - Eye Infections/*diagnosis/*microbiology
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - Metagenome
MH  - Molecular Diagnostic Techniques/*methods/trends
MH  - Polymerase Chain Reaction
MH  - Toxoplasma/genetics
MH  - Toxoplasmosis/complications/*diagnosis
EDAT- 2016/10/18 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/09/02 06:00
PHST- 2016/10/18 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/09/02 06:00 [entrez]
AID - 10.1097/ICU.0000000000000311 [doi]
PST - ppublish
SO  - Curr Opin Ophthalmol. 2016 Nov;27(6):561-567. doi: 10.1097/ICU.0000000000000311.