PMID- 27467585
OWN - NLM
STAT- MEDLINE
DCOM- 20170808
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 7
DP  - 2016
TI  - MRI as a Novel In Vivo Approach for Assessing Structural Changes of Chlamydia
      Pathology in a Mouse Model.
PG  - e0160055
LID - 10.1371/journal.pone.0160055 [doi]
AB  - Chlamydia trachomatis is among the most prevalent of sexually transmitted
      diseases. While Chlamydia infection is a reportable event and screening has
      increased over time, enhanced surveillance has not resulted in a reduction in the
      rate of infections, and Chlamydia infections frequently recur. The development of
      a preventative vaccine for Chlamydia may be the only effective approach for
      reducing infection and the frequency of pathological outcomes. Current vaccine
      research efforts involve time consuming and/or invasive approaches for assessment
      of disease state, and MRI presents a clinically translatable method for assessing
      infection and related pathology both quickly and non-invasively. Longitudinal
      T2-weighted MRI was performed over 63 days on both control or Chlamydia muridarum
      challenged mice, either with or without elementary body (EB) immunization, and
      gross necropsy was performed on day 65. A scoring system was developed to assess 
      the number of regions affected by Chlamydia pathology and was used to document
      pathology over time and at necropsy. The scoring system documented increasing
      incidence of pathology in the unimmunized and challenged mice (significantly
      greater compared to the control and EB immunized-challenged groups) by 21 days
      post-challenge. No differences between the unchallenged and EB
      immunized-challenged mice were observed. MRI scores at Day 63 were consistently
      higher than gross necropsy scores at Day 65, although two of the three groups of 
      mice showed no significant differences between the two techniques. In this work
      we describe the application of MRI in mice for the potential evaluation of
      disease pathology and sequelae caused by C. muridarum infection and this
      technique's potential for evaluation of vaccines for Chlamydia.
FAU - Hines, Catherine D G
AU  - Hines CD
AUID- ORCID: http://orcid.org/0000-0001-9826-3164
AD  - Department of Translational Imaging Biomarkers, MRL (West Point, PA), Merck &
      Co., Inc., Kenilworth, New Jersey, United States of America.
FAU - Wang, Shubing
AU  - Wang S
AD  - Department of Biometrics Research, MRL (Rahway, NJ), Merck & Co., Inc.,
      Kenilworth, New Jersey, United States of America.
FAU - Meng, Xiangjun
AU  - Meng X
AD  - Department of Translational Imaging Biomarkers, MRL (West Point, PA), Merck &
      Co., Inc., Kenilworth, New Jersey, United States of America.
FAU - Skinner, Julie M
AU  - Skinner JM
AD  - Department of Vaccines Early Discovery, MRL (West Point, PA), Merck & Co., Inc., 
      Kenilworth, New Jersey, United States of America.
FAU - Heinrichs, Jon H
AU  - Heinrichs JH
AD  - Global Project Leadership, Sanofi Pasteur, Swiftwater, Pennsylvania, United
      States of America.
FAU - Smith, Jeffrey G
AU  - Smith JG
AD  - Pharmaceutical Sciences Analytical Research & Development, Pfizer, Andover,
      Massachusetts, United States of America.
FAU - Boddicker, Melissa A
AU  - Boddicker MA
AD  - Department of Vaccines Early Discovery, MRL (West Point, PA), Merck & Co., Inc., 
      Kenilworth, New Jersey, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20160728
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Chlamydia Infections/*diagnostic imaging/microbiology
MH  - *Disease Models, Animal
MH  - HeLa Cells
MH  - Humans
MH  - Magnetic Resonance Imaging
MH  - Mice
PMC - PMC4965011
EDAT- 2016/07/29 06:00
MHDA- 2017/08/09 06:00
CRDT- 2016/07/29 06:00
PHST- 2016/04/01 00:00 [received]
PHST- 2016/07/13 00:00 [accepted]
PHST- 2016/07/29 06:00 [entrez]
PHST- 2016/07/29 06:00 [pubmed]
PHST- 2017/08/09 06:00 [medline]
AID - 10.1371/journal.pone.0160055 [doi]
AID - PONE-D-16-13316 [pii]
PST - epublish
SO  - PLoS One. 2016 Jul 28;11(7):e0160055. doi: 10.1371/journal.pone.0160055.
      eCollection 2016.