PMID- 27336691
OWN - NLM
STAT- MEDLINE
DCOM- 20170724
LR  - 20170724
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 6
DP  - 2016
TI  - Contribution of Staphylococcus aureus Coagulases and Clumping Factor A to Abscess
      Formation in a Rabbit Model of Skin and Soft Tissue Infection.
PG  - e0158293
LID - 10.1371/journal.pone.0158293 [doi]
AB  - Staphylococcus aureus produces numerous factors that facilitate survival in the
      human host. S. aureus coagulase (Coa) and von Willebrand factor-binding protein
      (vWbp) are known to clot plasma through activation of prothrombin and conversion 
      of fibrinogen to fibrin. In addition, S. aureus clumping factor A (ClfA) binds
      fibrinogen and contributes to platelet aggregation via a fibrinogen- or
      complement-dependent mechanism. Here, we evaluated the contribution of Coa, vWbp 
      and ClfA to S. aureus pathogenesis in a rabbit model of skin and soft tissue
      infection. Compared to skin abscesses caused by the Newman wild-type strain,
      those caused by isogenic coa, vwb, or clfA deletion strains, or a strain
      deficient in coa and vwb, were significantly smaller following subcutaneous
      inoculation in rabbits. Unexpectedly, we found that fibrin deposition and abscess
      capsule formation appear to be independent of S. aureus coagulase activity in the
      rabbit infection model. Similarities notwithstanding, S. aureus strains deficient
      in coa and vwb elicited reduced levels of several proinflammatory molecules in
      human blood in vitro. Although a specific mechanism remains to be determined, we 
      conclude that S. aureus Coa, vWbp and ClfA contribute to abscess formation in
      rabbits.
FAU - Malachowa, Natalia
AU  - Malachowa N
AD  - Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of
      Allergy and Infectious Diseases, National Institutes of Health, Hamilton,
      Montana, United States of America.
FAU - Kobayashi, Scott D
AU  - Kobayashi SD
AD  - Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of
      Allergy and Infectious Diseases, National Institutes of Health, Hamilton,
      Montana, United States of America.
FAU - Porter, Adeline R
AU  - Porter AR
AD  - Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of
      Allergy and Infectious Diseases, National Institutes of Health, Hamilton,
      Montana, United States of America.
FAU - Braughton, Kevin R
AU  - Braughton KR
AD  - Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of
      Allergy and Infectious Diseases, National Institutes of Health, Hamilton,
      Montana, United States of America.
FAU - Scott, Dana P
AU  - Scott DP
AD  - Rocky Mountain Veterinary Branch, Rocky Mountain Laboratories, National Institute
      of Allergy and Infectious Diseases, National Institutes of Health, Hamilton,
      Montana, United States of America.
FAU - Gardner, Donald J
AU  - Gardner DJ
AD  - Rocky Mountain Veterinary Branch, Rocky Mountain Laboratories, National Institute
      of Allergy and Infectious Diseases, National Institutes of Health, Hamilton,
      Montana, United States of America.
FAU - Missiakas, Dominique M
AU  - Missiakas DM
AD  - Department of Microbiology, University of Chicago, Chicago, Illinois, United
      States of America.
FAU - Schneewind, Olaf
AU  - Schneewind O
AD  - Department of Microbiology, University of Chicago, Chicago, Illinois, United
      States of America.
FAU - DeLeo, Frank R
AU  - DeLeo FR
AD  - Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of
      Allergy and Infectious Diseases, National Institutes of Health, Hamilton,
      Montana, United States of America.
LA  - eng
GR  - R01 AI052474/AI/NIAID NIH HHS/United States
GR  - R01 AI110937/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20160623
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Bacterial Proteins)
RN  - 0 (ClfA protein, Staphylococcus aureus)
RN  - 0 (Coagulase)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Abscess/*microbiology/pathology
MH  - Animals
MH  - Bacterial Proteins/metabolism
MH  - Coagulase/*metabolism
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Inflammation Mediators/metabolism
MH  - Rabbits
MH  - Soft Tissue Infections/*microbiology/pathology
MH  - Staphylococcal Skin Infections/*microbiology/pathology
MH  - Staphylococcus aureus/*metabolism/*pathogenicity
PMC - PMC4918888
EDAT- 2016/06/24 06:00
MHDA- 2017/07/25 06:00
CRDT- 2016/06/24 06:00
PHST- 2016/04/01 00:00 [received]
PHST- 2016/06/13 00:00 [accepted]
PHST- 2016/06/24 06:00 [entrez]
PHST- 2016/06/24 06:00 [pubmed]
PHST- 2017/07/25 06:00 [medline]
AID - 10.1371/journal.pone.0158293 [doi]
AID - PONE-D-16-13304 [pii]
PST - epublish
SO  - PLoS One. 2016 Jun 23;11(6):e0158293. doi: 10.1371/journal.pone.0158293.
      eCollection 2016.