PMID- 27093427
OWN - NLM
STAT- MEDLINE
DCOM- 20160829
LR  - 20170220
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 4
DP  - 2016
TI  - Differences in Whole Blood Gene Expression Associated with Infection Time-Course 
      and Extent of Fetal Mortality in a Reproductive Model of Type 2 Porcine
      Reproductive and Respiratory Syndrome Virus (PRRSV) Infection.
PG  - e0153615
LID - 10.1371/journal.pone.0153615 [doi]
AB  - Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant
      females causes fetal death and increased piglet mortality, but there is
      substantial variation in the extent of reproductive pathology between individual 
      dams. This study used RNA-sequencing to characterize the whole blood
      transcriptional response to type 2 PRRSV in pregnant gilts during the first week 
      of infection (at 0, 2, and 6 days post-inoculation), and attempted to identify
      gene expression signatures associated with a low or high level of fetal mortality
      rates (LFM and HFM; n = 8/group) at necropsy, 21 days post-inoculation. The
      initial response to infection measured at 2 days post-inoculation saw an
      upregulation of genes involved in innate immunity, such as interferon-stimulated 
      antiviral genes and inflammatory markers, and apoptosis. A concomitant decrease
      in expression of protein synthesis and T lymphocyte markers was observed. By day 
      6 the pattern had reversed, with a drop in innate immune signaling and an
      increase in the expression of genes involved in cell division and T cell
      signaling. Differentially expressed genes (DEGs) associated with extremes of
      litter mortality rate were identified at all three time-points. Among the 15 DEGs
      upregulated in LFM gilts on all three days were several genes involved in
      platelet function, including integrins ITGA2B and ITGB3, and the chemokine PF4
      (CXCL4). LFM gilts exhibited a higher baseline expression of
      interferon-stimulated and pro-inflammatory genes prior to infection, and of T
      cell markers two days post-infection, indicative of a more rapid progression of
      the immune response to PRRSV. This study has increased our knowledge of the early
      response to PRRSV in the blood of pregnant gilts, and could ultimately lead to
      the development of a biomarker panel that can be used to predict PRRSV-associated
      reproductive pathology.
FAU - Wilkinson, Jamie M
AU  - Wilkinson JM
AD  - Department of Agricultural, Food, and Nutritional Science, University of Alberta,
      Edmonton, AB, Canada.
FAU - Ladinig, Andrea
AU  - Ladinig A
AD  - Department of Large Animal Clinical Sciences, Western College of Veterinary
      Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Bao, Hua
AU  - Bao H
AD  - Department of Agricultural, Food, and Nutritional Science, University of Alberta,
      Edmonton, AB, Canada.
FAU - Kommadath, Arun
AU  - Kommadath A
AD  - Department of Agricultural, Food, and Nutritional Science, University of Alberta,
      Edmonton, AB, Canada.
FAU - Stothard, Paul
AU  - Stothard P
AD  - Department of Agricultural, Food, and Nutritional Science, University of Alberta,
      Edmonton, AB, Canada.
FAU - Lunney, Joan K
AU  - Lunney JK
AD  - Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center,
      Agricultural Research Service, U.S. Department of Agriculture, Beltsville,
      Maryland, United States of America.
FAU - Harding, John C S
AU  - Harding JC
AD  - Department of Large Animal Clinical Sciences, Western College of Veterinary
      Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Plastow, Graham S
AU  - Plastow GS
AD  - Department of Agricultural, Food, and Nutritional Science, University of Alberta,
      Edmonton, AB, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160419
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Blood Cells/immunology/*virology
MH  - Female
MH  - Fetal Death
MH  - Fetal Mortality
MH  - Gene Expression/*genetics
MH  - Immunity, Innate/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/immunology/*mortality
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/genetics/immunology/mortality
MH  - Reproduction/*genetics/immunology
MH  - Swine/immunology/*virology
MH  - T-Lymphocytes/immunology
PMC - PMC4836665
EDAT- 2016/04/20 06:00
MHDA- 2016/08/30 06:00
CRDT- 2016/04/20 06:00
PHST- 2016/02/04 00:00 [received]
PHST- 2016/03/31 00:00 [accepted]
PHST- 2016/04/20 06:00 [entrez]
PHST- 2016/04/20 06:00 [pubmed]
PHST- 2016/08/30 06:00 [medline]
AID - 10.1371/journal.pone.0153615 [doi]
AID - PONE-D-16-04940 [pii]
PST - epublish
SO  - PLoS One. 2016 Apr 19;11(4):e0153615. doi: 10.1371/journal.pone.0153615.
      eCollection 2016.